Investigating the Changes in the Antioxidant Activity of Honey in Different Storage Conditions

Fatemeh Gheitanchi^1, , Abolfazl Kamkar¹, Hessameddin Akbarein¹ and Sina Sakhaeifar¹

¹Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

Cite this paper:
Fatemeh Gheitanchi, Abolfazl Kamkar, Hessameddin Akbarein and Sina Sakhaeifar. Investigating the Changes in the Antioxidant Activity of Honey in Different Storage Conditions. American Journal of Food Science and Technology. 2021; 9(2):38-42. doi: 10.12691/ajfst-9-2-2

Abstract

Honey is a naturally sweet substance that the bee collects from the nectar of flowers and sap of plants, after adding various enzymes, processing and evaporating excess moisture, it stores it in the hive. The aim of this study is investigating the changes in the antioxidant activity of the 10 honey samples under different storage conditions [The samples were heated in water bath (48 Degree Celsius for 1 day and 80 Degree Celsius for 4 minutes), or kept at room temperature (25 Degree Celsius) for 3 and 6 months. Then they were re- evaluated for antioxidant activity]. Antioxidant activity by three methods: DPPH, beta-carotene-linoleic acid and reduction power method in honey samples evaluated. Measurements for all methods used in this research were done in three replications for each sample. Data were analyzed by SPSS software. Kolmogorov-Smirnov test was used to check the normality of the distribution of variables. The one-way ANOVA was used to compare the mean between groups. According to the results, antioxidant activity of honeys were increased under thermal condition and were decreased during storage. Storage and processing condition (thermal treatment) can cause changes in antioxidant activity as well as the quality of honey.

Keywords:
honey antioxidant activity DPPH reduction power storage

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References:

[1]	Alzahrani, H. A., Alsabehi, R., Boukraâ, L., Abdellah, F., Bellik, Y., & Bakhotmah, B. A. (2012). Antibacterial and antioxidant potency of floral honeys from different botanical and geographical origins. Molecules, 17(9), 10540-10549.
[2]	Lakzade, L. Qaysari, H. Mahianeh, A. (2013) Comparison of Physicochemical and Microbial Properties of Honey of Different Vegetable Origin in Isfahan Province, Veterinary Research and Construction Journal, No. 100. Pages 30-23.
[3]	Samarghandian, S., Farkhondeh, T., & Samini, F. (2017). Honey and health: A review of recent clinical research. Pharmacognosy research, 9(2), 121.
[4]	Baglio, E. (2018). Honey: processing techniques and treatments. In Chemistry and Technology of Honey Production (pp. 15-22). Springer, Cham.
[5]	Ouchemoukh, S., Louaileche, H., & Schweitzer, P. (2007). Physicochemical characteristics and pollen spectrum of some Algerian honeys. Food control, 18(1), 52-58.
[6]	Saxena, S., Gautam, S., & Sharma, A. (2010). Physical, biochemical and antioxidant properties of some Indian honeys. Food chemistry, 118(2), 391-397.
[7]	Viuda-Martos, M., Ruiz-Navajas, Y., Zaldivar-Cruz, J. M., Kuri, V., Fernández-López, J., Carbonell-Barrachina, Á. A., & Pérez-Álvarez, J. (2010). Aroma profile and physic-chemical properties of artisanal honey from Tabasco, Mexico. International journal of food science & technology, 45(6), 1111-1118.
[8]	Vela, L., de Lorenzo, C., & Perez, R. A. (2007). Antioxidant capacity of Spanish honeys and its correlation with polyphenol content and other physicochemical properties. Journal of the Science of Food and Agriculture, 87(6), 1069-1075.
[9]	Isla, M. I., Craig, A., Ordoñez, R., Zampini, C., Sayago, J., Bedascarrasbure, E., & Maldonado, L. (2011). Physico chemical and bioactive properties of honeys from Northwestern Argentina. LWT-Food Science and Technology, 44(9), 1922-1930.
[10]	Ahmed, M., Khiati, B., Meslem, A., Aissat, S., & Djebli, N. (2014). Evaluation of physicochemical and antioxidant properties of raw honey from Algeria. J Microbial Biochem Technol. S, 4.
[11]	Waston, D., H. (2002). Food chemical safety V2: Additives. Cambridge, England: Woodhead Publishing Limited, 283-85.
[12]	Boussaid, A., Chouaibi, M., Rezig, L., Hellal, R., Donsì, F., Ferrari, G., & Hamdi, S. (2018). Physicochemical and bioactive properties of six honey samples from various floral origins from Tunisia.Arabian Journal of Chemistry, 11(2), 265-274.
[13]	Gomes, S., Dias, L. G., Moreira, L. L., Rodrigues, P., & Estevinho, L. (2010). Physicochemical, microbiological and antimicrobial properties of commercial honeys from Portugal. Food and Chemical Toxicology, 48(2), 544-548.
[14]	Socha, R., Juszczak, L., Pietrzyk, S., Gałkowska, D., Fortuna, T., & Witczak, T. (2011). Phenolic profile and antioxidant properties of Polish honeys. International journal of food science & technology, 46(3), 528-534.
[15]	Iritie, B. M., Wandan, E. N., Yapo, M. Y., Fantodji, A., & Bodji, N. C. (2014). Comparative analysis of physico-chemical characteristics of honeys produced in the multi-floral arboretum of the national school of agronomy of Yamoussoukro. International Journal of Agricultural Policy and Research, 2(11), 379-382.
[16]	Alvarez-Suarez, J. M., Tulipani, S., Díaz, D., Estevez, Y., Romandini, S., Giampieri, F., Battino, M. (2010). Antioxidant and antimicrobial capacity of several monofloral Cuban honeys and their correlation with color, polyphenol content and other chemical compounds. Food and Chemical Toxicology, 48(8-9), 2490-2499.
[17]	Bertoncelj, J., Doberšek, U., Jamnik, M., & Golob, T. (2007). Evaluation of the phenolic content, antioxidant activity and colour of Slovenian honey. Food Chemistry, 105(2), 822-828.
[18]	Moniruzzaman, M., Sulaiman, S. A., Azlan, S. A. M., & Gan, S. H. (2013). Two-year variations of phenolics, flavonoids and antioxidant contents in acacia honey. Molecules, 18(12), 14694-14710.
[19]	Šarić, G., Marković, K., Major, N., Krpan, M., Uršulin-Trstenjak, N., Hruškar, M., & Vahčić, N. (2012). Changes of antioxidant activity and phenolic content in acacia and multifloral honey during storage. Food Technology and Biotechnology, 50(4), 434-441.
[20]	ISIRI 92, (1386), Honey-Specification and test methods, 6th Revision, Institute of Standards and Industial Research of Iran, Tehran, 1-24.
[21]	Ferreira, I. C., Aires, E., Barreira, J. C., & Estevinho, L. M. (2009). Antioxidant activity of Portuguese honey samples: Different contributions of the entire honey and phenolic extract. Food Chemistry, 114(4), 1438-1443.
[22]	Dong, R., Zheng, Y., & Xu, B. (2013). Phenolic profiles and antioxidant capacities of Chinese unifloral honeys from different botanical and geographical sources. Food and bioprocess technology, 6(3), 762-770.
[23]	Perna, A., Simonetti, A., Intaglietta, I., & Gambacorta, E. (2013). Antioxidant properties, polyphenol content and colorimetric characteristics of different floral origin honeys from different areas of Southern Italy. Journal of Life Sciences, 7(4), 428-436.
[24]	Pichichero, E., Canuti, L., & Canini, A. (2009). Characterisation of the phenolic and flavonoid fractions and antioxidant power of Italian honeys of different botanical origin. Journal of the Science of Food and Agriculture, 89(4), 609-616.
[25]	Sowndhararajan, K., Joseph, J. M., Arunachalam, K., & Manian, S. (2010). Evaluation of Merremia tridentata (L.) Hallier f. for in vitro antioxidant activity. Food Science and Biotechnology, 19(3), 663-669.
[26]	Turkmen, N., Sari, F., Poyrazoglu, E. S., & Velioglu, Y. S. (2006). Effects of prolonged heating on antioxidant activity and colour of honey. Food Chemistry, 95(4), 653-657.