American Journal of Food Science and Technology
ISSN (Print): 2333-4827 ISSN (Online): 2333-4835 Website: Editor-in-chief: Hyo Choi
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American Journal of Food Science and Technology. 2020, 8(5), 185-195
DOI: 10.12691/ajfst-8-5-3
Open AccessArticle

Analysis of the Flavor and Active Compounds in Lonicera japonica Wine Produced by a New Extraction and Fermentation Method

Xiaolong Zhou1, Orlando Borras-Hidalgo1, Wenting Ruan2, Xinli Liu1, 3, and Guoxiang Lin1

1State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan, China

2Environmental Microbiology Laboratory, Chung-Ang University, Seoul, Korea

3Shandong Provincial Key Laboratory of Microbial Engineering, Department of Bioengineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan, China

Pub. Date: September 29, 2020

Cite this paper:
Xiaolong Zhou, Orlando Borras-Hidalgo, Wenting Ruan, Xinli Liu and Guoxiang Lin. Analysis of the Flavor and Active Compounds in Lonicera japonica Wine Produced by a New Extraction and Fermentation Method. American Journal of Food Science and Technology. 2020; 8(5):185-195. doi: 10.12691/ajfst-8-5-3


Lonicera japonica species have been used as an adjuvant therapy for physical fitness, due to their extensive biological activity and pharmaceutical properties. For example, this species demonstrates antimicrobial, anti-inflammatory and detoxifying effects. Therefore, the changes in the chlorogenic acid, amino acid, and total polyphenol contents and in the antioxidant activity were analyzed. The results showed that the content of chlorogenic acid was 2378.63 mg/L without residue fermentation, 2039.05 mg/L with residue fermentation and 1476.74 mg/L with liquor extraction, which were increases of 1.86, 1.53 and 0.87 times more, respectively, than the chlorogenic content in unfermented L. japonica broth. The total content of polyphenols during the fermentation time had a positive linear correlation and the following values were obtained; fermentation without residue was 0.73 mg/mL, fermentation with residue was 0.62 mg/mL, and liquor extraction was 0.45 mg/mL. The clearance rate of ABTS and DPPH reached approximately 95%. Moreover, flowers could be used to avoid the production of methanol and fusel oil. Therefore, the nutrients and active functional components in L. japonica and the flower flavor of L. japonica could be preserved. This suggested fermentation process is better than the liquor extraction process and is a safe and controllable metabolic process.

Lonicera japonica chlorogenic acid polyphenol antioxidant activity liquid fermentation

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[1]  Liu Q, Fang J, Wang P, Du Z, Li Y, Wang S, & Ding K. 2017. Characterization of a pectin from LonicerajaponicaThunb and its inhibition effect on Aβ42 aggregation and promotion of neuritogenesis. Int.J.Biol. Macromol: 107, 112.
[2]  Wang D-Y, Zhao X-M, & Liu Y-L. 2017. Hypoglycemic and hypolipidemic effects of a polysaccharide from flower buds of Lonicera japonica in streptozotocin-induced diabetic rats.Int. J. Biol. Macromol. 102: 396-404.
[3]  Jiang B, Hao Z-S, Wu W-D, Li Z-S, & Zhang J-R. 2018. A new PAT application: Optimization of processing methods for honeysuckle flower (LoniceraeJaponicaeFlos) and wild honeysuckle flower. Journal of Traditional Chinese Medical Sciences 7:199-205.
[4]  Li J, Jin S-Y, Zu G-M, Luo W, Wang C-J, Zhao Y-J, & Fu Y-J. 2013. Rapid preparative extraction and determination of major organic acids in honeysuckle (Lonicera japonica Thunb.) tea.J. Food Anal 33:139-145.
[5]  Liu Z-X, Cheng Z-Y, He Q-J, Lin B, Gao P-Y, Li L-Z, & Liu Q-B, Song S-J. 2016. Secondary metabolites from the flower buds of Lonicera japonica and their in vitro anti-diabetic activities. Fitoterapia 110:44-51.
[6]  Han J, Lv Q-Y, Jin S-Y, Zhang T-T, Jin S-X, Li X-Y,&Yuan H-L. 2014. Comparison of anti-bacterial activity of three types of di-O-caffeoylquinic acids in Lonicera japonica flowers based on microcalorimetry. Chinese Journal of Natural Medicines 12(2): 108-113.
[7]  Fan, Z-L , Li L , Bai X-L , Zhang H , Liu Q-R , Zhang H , Fu, Y-J, &Moyo R . 2019. Extraction optimization, antioxidant activity, and tyrosinase inhibitory capacity of polyphenols from Lonicera japonica. FOOD SCIENCE & NUTRITION 7(5): 1786-1794.
[8]  Lee Y-S , Lee Y-J ,&Park, S-N .2018. Synergistic Antimicrobial Effect of Lonicera japonica and Magnolia obovata Extracts and Potential as a Plant-Derived Natural Preservative. JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY 28(11): 1814-1822.
[9]  Xin N, Li W , Li Y-J ,Ma X-K , & Fu Z-P . 2011. Study of antivirus, antibacteria and immune functions of Gaoreqing freeze-dried powder. JOURNAL OF MEDICINAL PLANTS RESEARCH 5(22): 5407-5412.
[10]  PalikovaI ,Valentova K , Oborna I, &Ulrichova J . 2009. Protectivity of Blue Honeysuckle Extract against Oxidative Human Endothelial Cells and Rat Hepatocyte Damage. Journal of Agricultural and Food Chemistry 57(15): 6584-6589.
[11]  Seo O-N, Kim G-S, Park S, Lee J-H, Kim Y-H, Lee W-S, Lee S-J, Kim C-Y, Jin J-S ,Choi S-K ,& Shin S-C. 2012. Determination of polyphenol components of Lonicera japonica Thunb. Using liquid chromatography–tandem mass spectrometry: contribution to the overall antioxidant activity. Food Chem 134 (1):572-577.
[12]  Kwak W-J, Han C-K, Chang H-W, Kim H-P, Kang S-S, & Son K-H. 2003. Loniceroside C, an anti‐inflammatory saponins from Lonicera japonica. Chemical & Pharmaceutical Bulletin 51: 333-335.
[13]  Shang X-F, Pan H, Li M-X, Miao X-L, &Ding H. 2011.Lonicera japonica Thunb.:Ethnopharmacology, phytochemistry and pharmacology of an important traditional Chinese medicine. Journal of Ethnopharmacology 138(1): 1-21.
[14]  He L, & Chen S-L. 2013. Research progress of chlorogenic acid synthesis pathway in plants. Pharmaceutical biotechnology: 20(05): 463-466.
[15]  RudkinG-O, & Nelson J-M. 1947. Chlorogenic acid and respiration of sweet potatoes. J Am Chem Soc 69: 1470.
[16]  Shi L-S, Mu T-H, & Sun H-N. 2014. Advances in chlorogenic acids at home and abroad. Journal of nuclear agriculture 28(02): 292-301.
[17]  Bassoli B-K, Cassolla P, & Borba-Murad G-R . 2008. Chlorogenic acid reduces the plasma glucose peak in the oral glucose tolerance test:effects on hepatic glucose release and glycaemia. Cell Biochemistry and Function 26 (3):320-328.
[18]  Dos Santos M-D, Almeida M-C, & Lopes N-P . 2006. Evaluation of the anti-inflammatory, analgesic and antipyretic activities of the natural polyphenol chlorogenic acid.Biological and Pharmaceutical Bulletin 29 (11): 2236-2240.
[19]  Yang X-F, Wang Y, & Ma Y-G. 2013. Extraction, separation and structural identification of chlorogenic acid from eucommiaulmoides leaves. Food research and development 34(04): 32-34.
[20]  Watanabe T, Arai Y, Mitsui Y, Kusaura T, Okawa W, Kajihara Y , & Saito I. 2006. The blood pressure-lowering effect and safety of chlorogenic acid from green coffee bean extract in essential hypertension. Clinical and Experimental Hypertension 28(5): 439449.
[21]  Wu Z-Y, Chen L-Y, Guo Z-H, Li K-Y, Fu Y-X, Zhu J-L, Chen X-T, Huang C, Zheng C-L, Ma Y-H, Li X-G, Zhou J, Wang Z-Z, Xiao W & Wang Y-H. Systems pharmacology uncovers serotonergic pathway mediated psychotherapeutic effects of Lonicerae Japonicae Flos. JOURNAL OF FUNCTIONAL FOODS 60.
[22]  Rodriguez de Sotillo D-V, Hadley M, &Sotillo J-E. 2005. Insulin receptor exon 11+/− is expressed in Zucker (fa/fa) rats, and chlorogenic acid modifies their plasma insulin and liver protein and DNA.The Journal of Nutritional Biochemistry 17(1): 63-71.
[23]  Nicasio P, Aguilar-Santamar A-L, &Arandae. 2005. Hypoglycemic effect and chlorogenic acid content in two Cecropia species. Phytotherapy Research 19 (8): 661-664.
[24]  Muhammad N, Ghulam J-K, & Fang F-X. 2017. Chlorogenic acid (CGA): A pharmacological review and call for furtherresearch. Biomedicine & Pharmacotherapy 97:67-74.
[25]  Lei W, Hai H-L, & Cao H-B. 2013. Development of multifunctional health wine of honeysuckle.Guangdong chemical industry 40(16): 29-30.
[26]  Zou R, You Y-M, Chen Z-X, Hu K, & Ran L. (2016). Effects of drying method on polyphenols and antioxidant activity of honeysuckle. Food science 37(05):78-83.
[27]  Ying G, Jun W-H, & Jian D. 2014. Study on brewing technology and stability of blue honeysuckle. Science and Technology of Food Industry: 2 206-209.
[28]  Akkarachaneeyakorn S &Tinrat S. 2015. Effects of types and amounts of stabilizers on physical and sensory characteristics of cloudy ready-to-drink mulberry fruit juice. Food Science & Nutrition 3(3): 213-220.
[29]  Wang C-Y, Liu Y-W, Jia J-Q, Sivakumar T-R, Fan T, &Gui Z-Z. 2013. Optimization of fermentation process for preparation of mulberry fruit wine by response surface methodology. African Journal of Microbiology Research 7: 227- 236.
[30]  Wang L, Sun X, Li F, Yu D, Liu X, Huang W, & Zhan J. 2015. Dynamic changes in phenolic compounds, colour and antioxidant activity of mulberry wine during alcoholic fermentation. Journal of Functional Foods 18:254-265.
[31]  Ivana G &Mirella N. 2015. Polyphenols content, phenolics profile and antioxidant activity of organic red wines produced without sulfur dioxide/sulfites addition in comparison to conventional red wines. Food Chemistry 179: 336-342.
[32]  Wu Y-X, Liu B, Chang Y-L, & Wang Q. (2014). Optimization of Modified Supercritical CO2 Extraction of Chlorogenic Acid from the Flower Buds of Lonicera japonica Thunb and Determination of Antioxidant Activity of the Extracts. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 38(4): 443-450.
[33]  Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, & Rice-Evans C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine 26(9-10): 1231-1237.
[34]  Yang B, Zhao M, Shi J, Yang N, & Jiang Y. 2008.Effect of ultrasonic treatment on the recovery and DPPH radical scavenging activity of polysaccharides from longan fruit pericarp. Food Chem 106(2): 685-690.
[35]  LiJ-J, ZhaoA-P, LiD-M,& HeY. (2019). Comparative study of the free amino acid compositions and contents in three different botanical origins of Coptis herb. BIOCHEMICAL SYSTEMATICS AND ECOLOGY 83:117-120.
[36]  Liu Y. 2016. Research on evaluation of health value of traditional Chinese yellow rice wine. University of jinan.
[37]  Ljevar A, Curko N, Tomasevic M, Radosevic K Srcek V-G, Ganic K-K. 2016. Phenolic composition, antioxidant capacity and in vitro cytotoxicity assessment of fruit wines.Food Technology and Biotechnology 54(2): 145-155.
[38]  Simonetti P, Pietta P, Testolin G. 1997. Polyphenol content and total antioxidant potential of selected Italian wines. Journal of Agricultural and Food Chemistry 45(4).
[39]  Wang W-L, Wen C, Guo Q-P, Duan Y-H, Li Y-H, He S-P, & Li F-N. 2017. Bioactivity and mechanism of chlorogenic acid. Chinese journal of animal nutrition 29(07): 2220-2227.
[40]  Meinhart A-D, Damin FM , Caldeirao L , Ferreira D-S, Tayse F, Teixeira J,& Godoy H-T .2017. Chlorogenic acid isomer contents in 100 plants commercialized in Brazil. Food Research International 99: 522-530.
[41]  Muhammad N, Ghulam J-K, & Fang F-X. 2017. Chlorogenic acid (CGA): A pharmacological review and call for furtherresearch. Biomedicine & Pharmacotherapy 97:67-74.
[42]  Hu M, Hu Z-J, Du L-D, Du J, Luo, Q-S, Xiong J-H. 2019. Establishment of cell suspension culture of Lonicera japonica Thunb and analysis its major secondary metabolites. INDUSTRIAL CROPS AND PRODUCTS137: 98-104.
[43]  Lee J, Kang Y-R , Kim Y-J , & Chang Y-H. 2019. Effect of high pressure and treatment time on nutraceuticals and antioxidant properties of Lonicera japonica Thunb. INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES 54:243-251.
[44]  Soufleros E, Bouloumpasi E, Tsarchopoulos C, et al. 2003. Primary amino acid profiles of Greek white wines and their use in classification according to variety, origin and vintage. Food Chemistry 80(2): 261-273.
[45]  Shen F, Niu X, Yang D, et al. 2010. Determination of amino acids in Chinese rice wine by fourier transform near-infrared spectroscopy. Journal of Agricultural and Food Chemistry 58 (17):9809-9816.
[46]  Aquino F-W, Boso L-M, Cardoso D-R, et al. 2008.Amino acids profile of sugar cane spirit (cachaa), rum, and whisky. Food Chemistry 108 (2):784-793.
[47]  Wu Q, Yu L, & Qiu J. 2014. Linalool attenuates lung inflammation induced by Pasteurellamultocida via activating Nrf-2 signaling pathway. International Immunopharmacology 21 (2): 456-463.
[48]  Ge L-L, Xiao L-Y, Wan H-Q, Li J-M, Lv K-P, Peng S-S, Zhou B-P, Li T-Y, & Zeng X-B. 2019. Chemical constituents from Lonicera japonica flower buds and their anti-hepatoma and anti-HBV activities. BIOORGANIC CHEMISTRY.