World Journal of Analytical Chemistry
ISSN (Print): 2333-1178 ISSN (Online): 2333-1283 Website: http://www.sciepub.com/journal/wjac Editor-in-chief: Raluca-Ioana Stefan-van Staden
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World Journal of Analytical Chemistry. 2013, 1(2), 18-22
DOI: 10.12691/wjac-1-2-1
Open AccessArticle

Development and Validation of Spectrophotometric Method for Determination of Penicillamine (PA) in Pharmaceutical Formulation Using 4-Choro-Nitrobenzo-2-Oxa-1, 3-Diazol (NBD-CL)

Abdalla Ahmed Elbashir1, and Amira Anwar Babikir Alfadil2

1University of Khartoum, Faculty of Science, Chemistry Department, Khartoum, Sudan

2University of Sudan for Science & Technology, Faculty of Science, Department of chemical Laboratory, Khartoum, Sudan

Pub. Date: April 19, 2013

Cite this paper:
Abdalla Ahmed Elbashir and Amira Anwar Babikir Alfadil. Development and Validation of Spectrophotometric Method for Determination of Penicillamine (PA) in Pharmaceutical Formulation Using 4-Choro-Nitrobenzo-2-Oxa-1, 3-Diazol (NBD-CL). World Journal of Analytical Chemistry. 2013; 1(2):18-22. doi: 10.12691/wjac-1-2-1

Abstract

A sensitive and simple spectrophotometric method has been proposed for the determination of D-Penicillamine (PA) in pharmaceutical formulations. The proposed method is based on the reaction between the PA and 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-CL) at alkaline medium (pH 10.5) to form deep brown-purple adduct, exhibiting maximum absorption (λmax) at 468nm. Under optimized reaction condition, the method was linear in the concentration range 1-15µg mL-1. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.11µg mL-1, 0.38µg mL-1, respectively. The method was applied successfully to the determination of PA in pharmaceutical dosage form. A proposal of the reaction pathway has been postulated. The results were in a good agreement with those obtained with the official USP method. The method is useful for routine analysis of PA in quality control laboratories.

Keywords:
D-Penicillamine spectrophotometric pharmaceutical formulation method validation NBD-CL

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