Applied Ecology and Environmental Sciences
ISSN (Print): 2328-3912 ISSN (Online): 2328-3920 Website: http://www.sciepub.com/journal/aees Editor-in-chief: Alejandro González Medina
Open Access
Journal Browser
Go
Applied Ecology and Environmental Sciences. 2021, 9(1), 79-82
DOI: 10.12691/aees-9-1-12
Open AccessArticle

Chromium VI Detoxification by P. fluorescens Adopted from Tannery Effluent under Optimal Condition

Nilofer Nisha H.M1, and P. Kavitha1

1Department of Microbiology, Srimad Andavan College of Arts and Science (Autonomous), Affiliated to Bharathidasan University, Tiruchirappalli, Tamilnadu, India

Pub. Date: December 15, 2020

Cite this paper:
Nilofer Nisha H.M and P. Kavitha. Chromium VI Detoxification by P. fluorescens Adopted from Tannery Effluent under Optimal Condition. Applied Ecology and Environmental Sciences. 2021; 9(1):79-82. doi: 10.12691/aees-9-1-12

Abstract

The present study is aimed to isolate and evaluate chromium reduction by the indigenous bacterium Pseudomonas fluorescens in tannery effluents collected from airport, Tiruchirappalli. Chromium resistant pattern was studied by plate method and reduction was studied by shake flask method. Isolate Pseudomonas fluorescens biochemically characterized as Gram negative siderophore producing bacteria and found to tolerate 1000 ppm Cr VI. Among the pH best growth was found at pH 6 showed Lag Growth phase was observed on 4 h at nonchromium condition and 6 h for chromium supplemented medium at 100 ppm and 7 h at 1000ppm. The growth was retreated by chromium but not arrested. The yield of the bio-surfactant was relatively higher in pH 8. Medium with yeast extract, pH 8 comparatively better than other nitrogen in chromium reduction. Maximum Chromium removal efficiency was 82% under chitosan and 78% by EDTA supplemented medium. Other nitrogen sources were less significant. In pH 8 under yeast extract and glucose with EDTA and 68% chromium reduction was noted without EDTA. Medium with chitosan showed enhanced reduction from 55 % to 88%. Other nitrogen sources like peptone, casein, ammonium nitrate showed 60-70% reduction in the presence of EDTA and reduction was reduced to 50% without EDTA. The increase of dextrose strength does not influence the reduction of chromium and maximum activity was found at 2% same as 10%.

Keywords:
Pseudomonas fluorescens Chromium EDTA Chitosan Reduction

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

References:

[1]  Ahluwalia, S. S., and Goyal, D. 2007. Microbial and plant derived biomass for removal of heavy metals from wastewater. Bioresource Technology, 98(12), pp. 2243-2257.
 
[2]  Agrawal, A., Kumar, V., and Pandey, B. D. 2006. Remediation options for the treatment of electroplating and leather tanning effluent containing chromium a review. Mineral Processing and Extractive Metallurgy Review, 27(2), pp. 99-130.
 
[3]  Moyo, S., Gashe, B. A., Collison, E. K., and Mpuchane, S. 2003. Optimising growth conditions for the pectinolytic activity of Kluyveromyces wickerhamii by using response surface methodology. International Journal of Food Microbiology, 85(1- 2), pp. 87-100.
 
[4]  Gupta, P. and Diwan, B. 2017. Bacterial exopolysaccharide mediated heavy metal removal a review on biosynthesis, mechanism and remediation strategies. Biotechnol. Rep, 13, 58-71.
 
[5]  Singh, A., Van Hamme, J. D., and Ward, O.P. 2007. Surfactants in microbiology and biotechnology. Application Aspects of Biotechnology Adv, 25, pp. 99-121.
 
[6]  Rufino, R.D., Luna, J.M., Campos-Takaki, G.M., Ferreira, S.R.M., and Sarubbo, L.A. 2012. Application of the biosurfactant produced by Candida lipolytica in the remediation of heavy metals. Chemical Engineering Transactions, 27, pp. 61-66.
 
[7]  Sen, S., Nandi, S. and Dutta, S. 2018. Application of RSM and ANN for optimization and modeling of biosorption of chromium (VI) using cyanobacterial biomass. Appl. Water Sci, 8(5), 148.
 
[8]  Wani, P.A., Wahid, S., Singh, R., Kehinde, A.M. 2018. Antioxidant and chromium reductase assisted chromium (VI) reduction and Cr (III) immobilization by the Rhizospheric Bacillus helps in the remediation of Cr (VI) and growth promotion of soybean crop Rhizosphere.
 
[9]  Souza, J.R.R., Feitosa, J.P.A., Ricardo, N.M.P.S., Trevisan, M.T.S., de Paula, H.C.B., Ulrich, C.M., Owen, R.W. 2013. Spray drying encapsulation of mangiferin using natural polymer. Food Hydrocoll, 33, pp. 10-18.
 
[10]  Zhang, L., Zeng, Y., and Cheng, Z. 2016. Removal of heavy metals ion using chitosan and modified chitosan a review. Journal of Molecular Liquids, 214, pp. 715-191.
 
[11]  Mujeeb Rahman, Shereen Gul, M. Z., and Ul Haq. 2007. Reduction of chromium by locally isolated Pseudomonas sp. C-171. Turkish Journal of Biology, 31, pp. 161-166.
 
[12]  Saravanan, A., Senthil Kumar, P., and Preetha, B. 2016. Optimization of process parameters for the removal of chromium (VI) and nickel (II) from aqueous solutions by mixed biosorbents (custard apple seeds and Aspergillus niger using response surface methodology. Desalination Water Treatment, 57(31), pp. 14530-14543.
 
[13]  Oner, E. T. 2013. Microbial production of extracellular polysaccharides from biomass. In Pretreatment techniques for biofuels and biorefineries, 35-56.
 
[14]  Rashmi Rekha Saikia., Suresh Deka., Manab Deka., and Ibrahim., Banat. R. 2012. Isolation of biosurfactant-producing Pseudomonas aeruginosa RS29 from oil-contaminated soil and evaluation of different nitrogen sources in biosurfactant production. Annals of Microbiology, 62, pp. 753-763.
 
[15]  Parameswari, E., Lakshmanan, A., Thilagavathi, T. 2009. Chromate resistance and reduction by bacterial isolates. Aust. J. Basic Appl. Science, 3, pp. 1363-1368.