Effect of Processing on the Nutrient, Anti-nutrient and Functional Properties of Mucuna flagellipes (Ox-eyed Bean) Seed Flour; An Underutilized Legume in Nigeria

Nwajagu I.U^1, , Garba A.¹, Nzelibe H.C¹, Chukwuekezie N.E², Abah C.R³, Umar A.T^{1, 4}, Anarado C.S³, Kahu J.C⁴, Olagunju A.¹, Oladejo A.A⁵ and Bashiru I.¹

¹Department of Biochemistry, Ahmadu Bello University Zaria Kaduna State, Nigeria

²Department of Water and Waste water Engineering, Cranfield University Bedfordshire, United Kingdom

³Department of Food Science and Technology, Nnamdi Azikiwe University Awka Anambra State, Nigeria

⁴NAFDAC Kaduna Laboratory Services, Kaduna State, Nigeria

⁵Department of Applied Biochemistry, Nnamdi Azikiwe University Awka Anambra State, Nigeria

Cite this paper:
Nwajagu I.U, Garba A., Nzelibe H.C, Chukwuekezie N.E, Abah C.R, Umar A.T, Anarado C.S, Kahu J.C, Olagunju A., Oladejo A.A and Bashiru I.. Effect of Processing on the Nutrient, Anti-nutrient and Functional Properties of Mucuna flagellipes (Ox-eyed Bean) Seed Flour; An Underutilized Legume in Nigeria. American Journal of Food and Nutrition. 2021; 9(1):49-59. doi: 10.12691/ajfn-9-1-7

Abstract

The promotion and consumption of underutilized legumes could help mitigate food insecurity and alleviate malnutrition in developing countries. In this study, the effect of processing on the nutritional, anti-nutritional and functional properties of Mucuna flagellipes seed flour was determined. The proximate composition of the samples showed that the flours had a range of 7.37 to 11.46 % moisture, 18.21 to 28.53% crude protein, 2.93 to 3.97% fat, 8.06 to 12.90% crude fibre, 2.74 to 4.69% ash, 38.45 to 59.09% carbohydrate, and 1446.34 to 1285.43KJ/100g energy. Profile of the amino acid showed that the lysine, leucine, tryptophan and aspartic acid in the unprocessed flour were significantly (P ≤ 0.05) reduced by roasting. The vitamin composition of the samples showed that vitamin A, vitamin C and vitamin E varied between; 0.15 to 3.46 mg/100g, 0.03 to 85.54 mg/100g, 3.41 to 14.24mg/100g respectively. The mineral composition of the flours showed that the calcium, potassium, phosphorus, magnesium, iron and zinc contents of the samples varied between 132.02 to 167.00mg/100g, 85.91 to 145.00mg/100g, 80.95 to 132.87mg/100g; 83.74 to 145.19mg/100g, 19.90 to 33.16mg/100g and 3.96 to 6.26mg/100g, respectively. The following mineral ratios were lower than the reference balance (ideal) and also lower than the minimum in the acceptable ideal range: Ca/Mg, Ca/K, Ca/P and [K ⁄(Ca+Mg)] for all the seed flours except for Ca/P mineral ratio whose value for the roasted seed flour falls within the acceptable ideal range. The mineral safety index showed that Ca, Mg, P and Zn were all lower than the standards for all the seed flours, whereas Fe was higher than the standard in all the seed flour. The anti-nutritional factors of the samples were significantly (P ≤ 0.05) reduced by autoclaving and roasting than by boiling and soaking methods. The calculated molar ratios of phytate:calcium, phytate:iron, phytate:zinc, oxalate:calcium and [Phytate][Calcium]/[Zinc] were below the critical value and this indicate that the bioavailability of calcium, iron, and zinc in the raw and processed seed flour could be high. The nutrient composition of the flour suggests that it has the potential to be used as both nutritional supplements and functional ingredients in the preparation of a number of baked and complementary food products.

Keywords:
Mucuna flagellipes nutrient anti-nutrient mineral safety index bioavailability

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References:

[1]	Obi, C. D. and Okoye, J. I. (2017). Effects of Boiling and Autoclaving on the Chemical Composition and Functional Properties of Mucuna Flagellipes Seed Flours. International Journal of innovative food, nutrition and sustainable Agriculture, 5(2): 18-24.
[2]	Igwenyi, I.O.S and Akubugwo, E.I (2010). Analysis of four seeds used as soup thickeners in the South-Eastern part of Nigeria. International Conference on Chemistry and Chemical Engineering.
[3]	Wargovich, M. J. (2000). Anticancer properties of fruits and vegetables. HortScience, 35(4), 573-575.
[4]	Vollmann, J. (2016) Soybean versus other food grain legumes: A critical appraisal of the United Nations international year of pulses 2016. Bodenkult. J. Land Manag. Food Environ., 67: 17-24.
[5]	Makeri, M.U., Abdulmannan, F., Ilowefah, M.A., Chiemela, C., Bala, S.M. and Muhammad, K. (2017) Comparative physicochemical, functional and structural characteristics of winged bean (Psophocarpus tetragonolobus DC) and soybean (Glycine max) protein isolates. J. Food Meas. Charact., 11: 835-846.
[6]	Janardhanan, K., Gurumoorthi, P. and Pugalenthi, M. (2003). Nutritional potential of five accessions of a South Indian tribal pulse, Mucuna pruriens var utilis I. The effect of processing methods on the content of l-dopa, phytic acid, and oligosaccharides. Tropical and subtropical agroecosystems, 1(2-3).
[7]	FAO (2011) Food standard programme. FAO/WHO Bulletin; 12:272-289.
[8]	Enwere, N. J. (1998). Foods of plant origin. Afro-Orbis Publications, Limited. Pp 2-20.
[9]	Bau, H. M., Villaume, C., Nicolas, J. P. and Méjean, L. (1997). Effect of germination on chemical composition, biochemical constituents and antinutritional factors of soya bean (Glycine max) seeds. Journal of the Science of Food and Agriculture, 73(1), 1-9.
[10]	Ngatchic, M.T.J., Njintang, Y.N., Oben, J.E. and Mbofung, C.M.F. (2013) Protein quality and antigrowth effect of protein isolate of Mucuna (Mucuna pruriens) and Canavalia (Canavalia ensiformis) seeds. Sch Acad J Biosci 1(5):183-191.
[11]	Obizoba, I.C (1990). Effects of germination, dehulling and cooking on the nutritional value of cowpea flour. Journal of food science 51(5): 1390-1397.
[12]	Tarek. A.E. (2002). Nutritional composition and antinutritional factors of chickpeas (Cicer arietinum L.) undergoing different cooking methods and germination. Plant Foods for Human Nutrition, 57(1), 83-97
[13]	Obiakor-Okeke, P. N., Chikwendu, J. N. and Anozie, T. (2014). Effect of Different Processing Methods on the Chemical, Functional and Microbial Properties of Mucuna sloanei Seeds (Ukpo). International Journal of Nutrition and Food Sciences 2014; 3 (6): 551, 559.
[14]	Udensi, E. A., Eke, O. and Ukachukwu, S. N. (2001). Effect of traditional processing on the physicochemical properties of Mucuna cochinchinensis and Mucuna Utilis flours. Journal Agriculture, Food Technology and Environment, 1, 133-137.
[15]	AOAC (2010) Official methods of analysis of the Association of Official Analytical Chemists (18th Edn). Washington DC.
[16]	Onwuka, G. I. (2005). Food analysis and instrumentation: theory and practice. Naphthalic prints, Surulere, Lagos, Nigeria, 219-230.
[17]	AOAC (2006). Official method of analysis. Association of Official Analytical Chemists. 18th edition Washington D.C USA Pp. 38-46.
[18]	Maria, M.Y., Justo, P., Julio, G., Javier, V., Francisco, M. and Manuel, A. (2004). Determination of tryptophan by high-performance liquid chromatography of alkaline hydrolysates with spectrophotometric detection. Food Chemistry 85(2): 317-320.
[19]	FAO/WHO. (1991). Protein quality evaluation. Report of Joint FAO/WHO Expert Consultation.
[20]	Amadi B, Nchekwube O, Amadi C, Ugbogu A. and Duru, M. (2013). Elemental, amino acid and phytochemical constituents of three different species of eggplant. Int. J. Med. Arom. Plants. 3(2): 200-203.
[21]	Watts, D.L. (2010). HTMA mineral ratios: A brief discussion of their clinical importance. Trace Elements Newsletter, 21(1): pp. 1-3.
[22]	Hatcock, J.N. (1985). Quantitative evaluation of vitamin safety. Pharmay Times: 104-113.
[23]	Kaushal, P., Kumar, V. and Sharma, H. K. (2012). Comparative study of physicochemical, functional, antinutritional and pasting properties of taro (Colocasia esculenta), rice (Oryza sativa) flour, pigeonpea (Cajanus cajan) flour and their blends. LWT-Food Science and Technology, 48(1), 59-68.
[24]	Nwafor, F. I., Egonu, S. N., Nweze, N. O., & Ohabuenyi, N. (2017). Effect of processing methods on the nutritional values and anti-nutritive factors of Adenanthera pavonina L.(Fabaceae) seeds. African Journal of Biotechnology, 16(3), 106-112.
[25]	Kumari, P. V. and Sangeetha, N. (2017a). Effect of processing and drying methods on the nutritional characteristic of the multi-cereals and legume flour. Journal of Food Processing and Technology, 8(4).
[26]	Alozie, Y. E., Iyam, M. A., Lawal, O., Udofia, U. and Ani, I. F. (2009b). Utilization of Bambara Groundnut Flour blends in bread production. Journal of Food Technology, 7(4), 111-114.
[27]	Santosa, B.A.S., Sudaryono, S. and Widowati, S. (2005) Technology evaluation of flour instant popcorn and quality. J Penelitian Pascapanen Pertanian 2: 66-75.
[28]	Adebowale, 0.0.Y., Adeyemi, A., and Oshodi, A. A. (2005). Variability in the physicochemical, nutritional and antinutritional attributes of six Mucuna species. Food chemistry, 89(1), 37-48.
[29]	Ndidi, U. S., Ndidi, C. U., Aimola, I. A., Bassa, O. Y., Mankilik, M. and Adamu, Z. (2014). Effects of processing (boiling and roasting) on the nutritional and antinutritional properties of bambara groundnuts (Vigna subterranea [L.] Verdc.) from Southern Kaduna, Nigeria. Journal of Food Processing, 2014.
[30]	Adeparusi, E. O. (2001). Effect of processing on the nutrients and anti‐nutrients of lima bean (Phaseolus lunatus L.) flour. Food/Nahrung, 45(2), 94-96.
[31]	Pugalenthi, M., Vadivel, V. and Janak, P. (2007) Comparative evaluation of protein quality of raw and differentially processed seeds of an underutilized food legumes, (Abrus precatorius L) livestock Research for Rural Development Article No. 168. Available in the online website. http://www.lrrd.Org/lrrd19/11/puga19168.htm.
[32]	Vadivel, V. Pugalenthi, M. and Megha, M. (2008) Biological evaluation of protein quality of raw and processed seeds of gila bean (Entada scandens benth.) J. Trop. Sub Strop. Agroecosystems 8: 125-133.
[33]	Agume A.S.N., Njintang, N.Y. and Mbofung, C.M. (2017). Effect of soaking and roasting on the physic-chemical and pasting properties of soybean flour. Foods.; 6: 12.
[34]	Ndidi, U. S., Ndidi, C. U., Olagunju, A., Muhammad, A., Billy, F. G., & Okpe, O. (2014). Proximate, antinutrients and mineral composition of raw and processed (Boiled and Roasted) Sphenostylis stenocarpa seeds from Southern Kaduna, Northwest Nigeria. International Scholarly Research Notices, 2014.
[35]	Enwereuzoh, R. O., Okafor, D. C., Uzoukwu, A. E., Ukanwoke, M. O., Nwakaudu, A. A. and Uyanwa, C. N. (2015). Flavour extraction from Monodora myristica and Tetrapleura tetraptera and production of flavoured popcorn from the extract. European Journal of Food Science and Technology, 3(2), p1.
[36]	Okorie, C. P. and Olasupo, N. A. (2013). Controlled fermentation and preservation of UGBA–an indigenous Nigerian fermented food. SpringerPlus, 2(1), 470.
[37]	Bressani, R. (1993a). Grain quality of common beans. Food Revision Interntional. 9: 217-179.
[38]	Hefnawy, T. H. (2011). Effect of processing methods on nutritional composition and anti-nutritional factors in lentils (Lens culinaris). Annals of Agricultural Sciences, 56(2), 57-61.
[39]	Stocker, R. and Frei, B.; Endogenous antioxidant defences in human blood plasma. In: Oxidative stress: oxidants and antioxidants, ed. H. Sies, pp. 213-243. London, UK, Academic Press, 1991
[40]	Ari, M.M., Ayanwale, B.A., Adama, T.Z. and Olatunji, E.A. (2012) Effects of Different Fermentation Methods on the Proximate Composition, Amino Acid Profile and Some Antinutritional Factors (ANFs) In Soyabeans (Glycine Max). Fermentation Technology and Bioengineering. 2. 6-13.
[41]	Pereira E. J., Carvalho L. M., Dellamora-Ortiz G. M., Cardoso F. S., Carvalho J. L. and Viana D. S. (2014). Effects of cooking methods on the iron and zinc contents in cowpea (Vigna unguiculata) to combat nutritional deficiencies in Brazil. Food Nutr Res.; 58.
[42]	Faber, M. and Laubscher, R. (2008). Seasonal availability and dietary intake of β-carotene-rich vegetables and fruit of 2-year-old to 5-year-old children in a rural South African setting growing these crops at household level. International Journal of Food Sciences and Nutrition, 59(1), 46-60.
[43]	Shils M. E. G and Young V. R (1988). Modern Nutrition in health and disease, Lea and Febiger, Philadelphia USA, In: Nutrition, eds Nieman, D.C, Butterworth, D.E, Nieman, C.N, 1992. WMC Brown Publisher, Dubuque, USA, Pp 276-282.
[44]	National Research Council (NRC), (1989). Food and Nutrition Board Recommended Dietary Allowances (10th Edition). National Academy Press Washington D.C.
[45]	Nieman D. C., Butterworth D. E., Nieman C. N (1992). Nutrition, Wm. C. Brown Publishers. Dubuque.
[46]	Olaleye A. A., Adeyeye E. I. and Adesina A. J. (2013). Chemical composition of bambara groundnut (V. subterranea L. Verdc) seed parts. Bangladesh J. Sci. Ind. Res. 48(3): 167-178.
[47]	Alagbaoso, S. O., Nwosu, J. N., Njoku, N. E., Umelo, M. C., Eluchie, C. and Agunwa, I. M. (2015). Effect of processing on the nutritional and anti-nutritional properties of Canavalia plagiosperma piper seeds. European Journal of Food Science and Technology, 3(3), 45-69.
[48]	Inyang Ufot Evanson and Eyo Edima Atanguma (2015). Effect of Soaking African Yam Bean Seeds in Unripe Plantain Peel Ash Solutions on the Nutrients, Anti-nutrients and Functional Properties of the Flour. Journal of Food and Nutrition Sciences. Vol. 3, No.4, pp. 147-151.
[49]	C.U. Igwe, C.O. Ibegbulem, L.A. Nwaogu, C.O. Ujowundu, G.N. Okwu, Calcium, Zinc andPhytate Interrelationships in Four Lesser-Known African Seeds Processed into Food Condiments. J. Adv. Chem. 4(2013) 288-294.
[50]	Fekadu, H., Beyene, F., and Desse, G. (2013). Effect of Traditional Processing Methods on Nutritional Composition and Anti-nutritional Factors of Anchote (Coccinia Abyssinica (Lam.) Cogn.) Tubers Grown in Western Ethiopia. Journal of Food Processing and Technology, 4, 249.
[51]	Woldegiorgis, A. Z., Abate, D., Haki, G. D., Ziegler, G. R., and Harvatine, K. J. (2015). Fatty acid profile of wild and cultivated edible mushrooms collected from Ethiopia. Journal of Nutrition and Food Sciences, 5(3), 1.
[52]	Hurrell, R. F., Reddy, M. B., Juillerat, M. A., and Cook, J. D. (2003). Degradation of phytic acid in cereal porridges improves iron absorption by human subjects. The American journal of clinical nutrition, 77(5), 1213-1219.
[53]	Siegenberg, D., Baynes, R. D., Bothwell, T. H., Macfarlane, B. J., Lamparelli, R. D., Car, N. G., and Mayet, F. (1991). Ascorbic acid prevents the dose-dependent inhibitory effects of polyphenols and phytates on nonheme-iron absorption. The American journal of clinical nutrition, 53(2), 537-541.
[54]	Bhandari, M. R., and Kawabata, J. (2004). Assessment of anti-nutritional factors and bioavailability of calcium and zinc in wild yam (Dioscorea spp.) tubers of Nepal. Food chemistry, 85(2), 281-287.
[55]	Woldegiorgis, A. Z., Abate, D., Haki, G. D., and Ziegler, G. R. (2014). Antioxidant property of edible mushrooms collected from Ethiopia. Food chemistry, 157, 30-36.
[56]	Morris, E. R., and Ellis, R. (1989). Usefulness of the dietary phytic acid/zinc molar ratio as an index of zinc bioavailability to rats and humans. Biological Trace Element Research, 19(1-2), 107-117.
[57]	Frontela, C., Scarino, M. L., Ferruzza, S., Ros, G., and Martínez, C. (2009). Effect of dephytinization on bioavailability of iron, calcium and zinc from infant cereals assessed in the Caco-2 cell model. World Journal of Gastroenterology: WJG, 15(16), 1977.
[58]	Obah, G., and T. V. Amusan. 2009. Nutritive value and antioxidant properties of cereal gruels produced from fermented maize and sorghum. Food Biotechnology. 23:17–31.
[59]	Adetuyi, F. O., Osagie, A. U., and Adekunle, A. T. (2011). Nutrient, antinutrient, mineral and zinc bioavailability of okra Abelmoschus esculentus (L) Moench Variety. American Journal of Food and Nutrition, 1(2), 49-54.
[60]	Oladipo, F. Y. and Nwokocha, L. M. (2011) Effect of Sida acuta and Carborous olitorius mucilages on the physico-chemcial properties of maize and sorghum starches. Asian J. Appl. Sci.4:514-523.
[61]	T. A. Shittu, A. Dixon, S. O. Awonorin, L. O. Sanni and B. Maziya-Dixon (2008). Bread from composite cassava-wheat flour. 11: Effect of cassava genotype and nitrogen fertilizer on bread quality. Food Res. Inter. 41: 569-578.
[62]	Iwe, M. O. and Onadipe, O. O. (2001). Effect of extruded full fat soy flour into sweet potato flour on functional properties of the mixture. J. Sustain. Agric. Environ. 3(1): 109-117.
[63]	Khattab, R. Y. and Arntfield, S. D. (2009). Functional properties of raw and processed canola meal. LWT – Food Science and Technology, 42, 1119-1124.
[64]	Karuna, D., Noel, G. and Dilip, K. (1996). Production and use of raw potato flour in Mauritian traditional foods. Food and nutrition bulletin. United Nation University 17(2).
[65]	Wilhelm, L.R., Dwayna, A.S. and Gerand, H.B. (2004). Introduction to problem solving skills in: Food and Process Engineering Technology. ASAE.
[66]	Onimawo, I. A. and Asugo, S. (2004). Effects of germination on the nutrient content and functional properties of pigeon pea flour. Journal of Food Science and Technology-Mysore, 41(2), 170-174.