Effects of Herbal Mixture Extract on Menopausal Hot Flashes and Pharmacokinetics in Ovariectomized Rat Models

Gyuok Lee¹, Jaeyong Kim¹, Huwan Kang¹, SungYoon Park¹, Junkee Hong², Joohyun Oh², Jimin Kim², Chansung Park², Yongwook Lee² and Chul-yung Choi^1,

¹Jeollanamdo Institute of Natural Resources Research, Jangheung-gun, Jeollanamdo, South Korea

²Herbal Hormone Research Institute, Naturalendo Tech Co., Ltd, Gyeonggi, South Korea

Cite this paper:
Gyuok Lee, Jaeyong Kim, Huwan Kang, SungYoon Park, Junkee Hong, Joohyun Oh, Jimin Kim, Chansung Park, Yongwook Lee and Chul-yung Choi. Effects of Herbal Mixture Extract on Menopausal Hot Flashes and Pharmacokinetics in Ovariectomized Rat Models. Journal of Food and Nutrition Research. 2018; 6(2):116-123. doi: 10.12691/jfnr-6-2-8

Abstract

This study was conducted to evaluate the effects of Cynanchum wilfordii Hemsley, Phlomis umbrosa Turcz., and Angelica gigas Nakai extract (CPAE) on the reduction of tail skin temperature (TST) in ovariectomized (OVX) rats. To evaluate TST reduction in ovariectomized rats, 125, 250, and 1000 mg/kg CPAE was administered to rats for 1 week. The measurement of TST after the induction of artificial stress revealed a significant temperature reduction effect in the CPAE administration group (p<0.05). The TST induced by the injection of yohimbine, to induce hot flashes, was found to decrease as the administered dose of CPAE increased from 10 min to 20min (125 and 250 mg/kg/day, p<0.05; 1000 mg/kg/day, p<0.01). In addition, in a drug interaction test between tamoxifen, an anti-estrogen drug, and CPAE, no significant difference was found between the pharmacokinetic (PK) profiles after the administration of tamoxifen only and the combination of tamoxifen and CPAE. We also found that CPAE did not affect CYP2d4 and CYP3a2, which affect tamoxifen metabolism, in a subsequent experiment on liver tissues extracted during the drug interaction test. In this study, we found that CPAE inhibited temperature increase on the tail skin of OVX rats, and therefore is effective in the improvement of hot flashes. CPAE may be potentially used for the improvement of hot flashes induced by the administration of tamoxifen.

Keywords:
Cynanchum wilfordii Cytochrome P450 Hot Flash Tail Skin Temperature Tamoxifen

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