Effect of Capsosiphon fulvescens on Ethanol-induced Liver Damage in HepG2 Cells over Expressing CYP2E1

Haneul Jo¹, Ok-Kyung Kim^{1, 2}, Ho-Geun Yoon³, Eungpil Kim⁴, Kyungmi Kim⁵, Yoo-Hyun Lee⁶, Kyung-Chul Choi⁷, Jeongmin Lee⁸, Jeongjin Park^{1, 2,} and Woojin Jun^{1, 2,}

¹Division of Food and Nutrition, Chonnam National University, Gwangju, South Korea

²Research Institute for Human Ecology, Chonnam National University, Gwangju, South Korea

³Department of Biochemistry and Molecular Biology, Brain Korea 21 PLUS Project for Medical Sciences, Yonsei University College of Medicine, Seoul, South Korea

⁴Marine Biotechnology Research Center, Wando, South Korea

⁵Department of Biofood Analysis, Korea Bio Polytechnic, Ganggyung, South Korea

⁶Department of Food and Nutrition, The University of Suwon, Suwon, South Korea

⁷Department of Biomedical Sciences and Department of Pharmacology, University of Ulsan College of Medicine, Seoul, South Korea

⁸Department of Medical Nutrition, Kyung Hee University, Yongin, South Korea

Cite this paper:
Haneul Jo, Ok-Kyung Kim, Ho-Geun Yoon, Eungpil Kim, Kyungmi Kim, Yoo-Hyun Lee, Kyung-Chul Choi, Jeongmin Lee, Jeongjin Park and Woojin Jun. Effect of Capsosiphon fulvescens on Ethanol-induced Liver Damage in HepG2 Cells over Expressing CYP2E1. Journal of Food and Nutrition Research. 2017; 5(7):510-517. doi: 10.12691/jfnr-5-7-9

Abstract

In the present study, the protective effects of 10% ethanol extract of Capsosiphon fulvescens (CFE10) against alcoholic liver damage were investigated in vitro using CYP2E1-overexpressing hepatocytes (HepG2/2E1). To determine whether CFE10 attenuated ethanol-induced cell death, we compared the viability of HepG2/2E1 cells treated with 250 mM ethanol in the presence or absence of CFE10. Cell viability significantly increased after treatment with CFE10 and ethanol compared with that of cells treated with only ethanol. Additionally, CFE10 inhibited ethanol-induced ROS formation and lipid peroxidation. We also found that CFE10 attenuated the mRNA expression of CYP2E1, as well as decreased ethanol-induced lipid droplets, through stimulation of the AMPK pathway. Based on these results, the protective effect of CFE10 extract from C. fulvescens against liver damage and fatty liver induced by ethanol may occur via the alleviation of oxidative stress.

Keywords:
capsosiphon fulvescens alcohol CYP2E1 reactive oxygen species liver damage

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