Reproductive Effects of 3-Monochloropropane-1, 2-diol on Mice Sperm Function and Early Embryonic Development In Vitro

Luona Wen¹, Jianxia Sun², Shun Bai¹, Yunfeng Hu¹, Shi Wu¹, Rui Jiao¹, Shiyi Ou¹ and Weibin Bai¹

¹Department of Food Science and Engineering, Department of Developmental and Regenerative Biology, Biopharmaceutical R&D Center, Jinan University, Guangzhou, China

²Faculty of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou, China

Cite this paper:
Luona Wen, Jianxia Sun, Shun Bai, Yunfeng Hu, Shi Wu, Rui Jiao, Shiyi Ou and Weibin Bai. Reproductive Effects of 3-Monochloropropane-1, 2-diol on Mice Sperm Function and Early Embryonic Development In Vitro. Journal of Food and Nutrition Research. 2015; 3(6):405-409. doi: 10.12691/jfnr-3-6-8

Abstract

3-Monochloropropane-1, 2-diol (3-MCPD) is a well-known chloropropanol that is synthesized during food processing contamination. While evaluating sperm quality, sperm penetration into oocytes and early embryonic development, the present study investigated the effects of 3-MCPD onmicereproduction in vitro. During thein vitro fertilization (IVF) process, zygotes and 2-cell embryos were incubated with 3-MCPD-supplemented medium until 8-cell embryo formation to evaluate the reproductive toxicity of 3-MCPD. Our data showed that compared with the control group, the fertilization rate and cleavage rate remarkably decreased in groups treated with 1.802 and 3.160 mM 3-MCPD. The percentage of 2-cell embryo and 4-cell embryo formation also significantly decreased (P < 0.05). Moreover, 8-cell embryos were not observed in any of the treated groups. Taken together, these results suggest that 3-MCPD exposure has hazardous effects on the micesperm fertilization ability as well as embryonic development.

Keywords:
3-Monochloropropane-1 2-diol; mouse sperm function; early embryonic development

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