Antioxidant Role and Hepatoprotective Effects of Carob (Ceratonia siliqua L.) Seeds against Ethanol-Induced Oxidative Stress in Rats

Mehmet Ali Temiz¹, Atilla Temur¹ and İsmail Çelik^2,

¹Department of Science, Education Faculty, Yuzuncu Yil University, Van, Turkey

²Department of Molecular Biology and Genetic, Science Faculty, Yuzuncu Yil University, Van, Turkey

Cite this paper:
Mehmet Ali Temiz, Atilla Temur and İsmail Çelik. Antioxidant Role and Hepatoprotective Effects of Carob (Ceratonia siliqua L.) Seeds against Ethanol-Induced Oxidative Stress in Rats. Journal of Food and Nutrition Research. 2015; 3(1):57-61. doi: 10.12691/jfnr-3-1-10

Abstract

The purpose of this research was to determine the effects of carob seeds (CS) concerning with hepatoprotective and antioxidant role against ethanol-induced oxidative stress (OS) in rats. Experiment was conduted as control, 20% ethanol, 15% CS and 20% ethanol + 15% CS groups. At the end of the 50-day exposure period of test groups, the hepatopreventive and antioxidant capacity were assessed by measuring level of serum enzymes such as aspartate aminotransferase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH); antioxidant defense systems, including superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione S-transferase (GST), glutathione reductase (GR) activities, reduced glutathione (GSH) levels and malondialdehyde (MDA) contents in the tissues of rats. According to results, while the levels of the serum enzymes increased in ethanol group compared with the control group whereas decreased in ethanol + CS-treated group compared with the ethanol group. Administration of CS supplementation restored the ethanol induced imbalance between malondialdehyde and fluctuated antioxidant system towards near normal particularly in the tissues. Finally, it was concluded that CS has a hepatoprotective effect and antioxidant capacity in rats with ethanol toxicity, probably acting by promoting the antioxidative defense systems.

Keywords:
carob seeds serum biomarkers antioxidant defense systems malondialdehyde rats

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