Oleanolic acid Separation From Grape Skins by Aqueous Two-phase Extraction and Estimate Its Antioxidant Activity

Xifeng Zhang¹, Fenqin Zhang¹, Guanghong Luo^2, , Shenghui Yang² and Danxia Wang²

¹The College of Agriculture and Biotechnology (CAB), Hexi University, Zhangye, P.R. China

²Kaiyuan Bio-tech Development Center, Hexi University, Zhangye, P.R. China

Cite this paper:
Xifeng Zhang, Fenqin Zhang, Guanghong Luo, Shenghui Yang and Danxia Wang. Oleanolic acid Separation From Grape Skins by Aqueous Two-phase Extraction and Estimate Its Antioxidant Activity. Journal of Food and Nutrition Research. 2015; 3(1):34-39. doi: 10.12691/jfnr-3-1-6

Abstract

The aim of the current study is to focus on separating the oleanolic acid (OA) from previously prepared aqueous two-phase extraction (ATPE) of grape skins. Several different influential extraction parameters, such as ethanol concentration (v/w), ammonium sulphate concentration (w/w), crude extract concentration (w/w), extraction temperature, and pH, were also investigated. The optimal differential partitioning of OA was achieved in a system (at pH4.0, temperature=25°C)composed of 23% (v/w) ethanol, 18% (w/w) ammonium sulphate, 8% (w/w) crude extract and 41% (w/w) water. The recovery of extracted OA from experiments was determined to be 93.54%. The antioxidant activity of the separated ATPE in relative to VC were, also, investigated in this study at the proper conditions.The ATPE extract showed a relatively high antioxidant ability compared with that of Vitamin C. This proposed extraction technique opens up new possibilities in extraction of other active ingredients in natural plants or biologic samples.

Keywords:
oleanolic acid Aqueous two-phase extraction (ATPE) Ethanol/ammonium sulphate Antioxidant activity

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