Journal of Food and Nutrition Research
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Journal of Food and Nutrition Research. 2024, 12(4), 182-195
DOI: 10.12691/jfnr-12-4-3
Open AccessArticle

Polygonatum Sibiricum Polysaccharides Alleviate CCl4-Induced Acute Liver Injury in Mice Via Reducing Inflammation and Oxidative Stress

Jiujiu Li1, 2, Ting Wang1, Fuding Zhou1, Changchun Xiao2, Zhengxiang Liu1, Jinchuan Yu1, Di Liang3, Guangjun Wang4, Changsheng Shao5, Peng Wang5, and Wenjun Chen1,

1Department of Nutrition and Food Hygiene, School of Public Health, Anhui Medical University, Hefei 230032, Anhui, China

2Hefei Center for Disease Control and Prevention, Hefei, 230041, Anhui, China

3Department of Gastroenterology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, Hefei 230001, Anhui, China

4School of Public Health, Anhui Medical University, Hefei 230032, Anhui, China

5CAS Key Laboratory of High Magnetic Field and Ion Beam Physical Biology, Hefei Institutes of Physical Science, Chinese Academy of Sciences (CAS), Hefei 230031, China

Pub. Date: April 17, 2024

Cite this paper:
Jiujiu Li, Ting Wang, Fuding Zhou, Changchun Xiao, Zhengxiang Liu, Jinchuan Yu, Di Liang, Guangjun Wang, Changsheng Shao, Peng Wang and Wenjun Chen. Polygonatum Sibiricum Polysaccharides Alleviate CCl4-Induced Acute Liver Injury in Mice Via Reducing Inflammation and Oxidative Stress. Journal of Food and Nutrition Research. 2024; 12(4):182-195. doi: 10.12691/jfnr-12-4-3


From liver injury to hepatocirrhosis and hepatocarcinoma, irreversible progression correlates closely with inflammation. Polygonatum sibiricum has been reported to be beneficial to the liver, but its regulation of inflammation in liver injury has not been determined. Thus, we hypothesize that Polygonatum sibiricum polysaccharides (PSP) play a protective role against acute liver injury by inhibiting inflammation and explore the underlying mechanism. The concentration and constituents of polysaccharides in PSP were identified first. We found that, like bifendate, PSP pretreatment significantly ameliorated acute CCl4 exposure-induced liver injury and associated biomarkers. Meanwhile, PSP markedly attenuated inflammation and macrophage proinflammatory polarization both in the livers of CCl4-treated mice and LPS-treated Raw264.7 cells. Mechanistic investigation showed that PSP pretreatment markedly attenuated the activation of JAK2/STAT3/NF-κB signaling pathway in liver injury. Our findings demonstrated a novel understanding of PSP-mediated macrophage proinflammatory polarization in acute injury, which provides new knowledge regarding its application in acute liver injury treatment.

Polygonatum sibiricum macrophage M1 polarization acute liver injury inflammation JAK2/STAT3

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