Ca²⁺ and ABA on the Accumulation of GABA and Flavonoids in Germinated Salicornia bigelovii Torr. under NaCl Stress

Dujun Wang¹, Jie Wang¹, Jingying Zheng¹, Yueling Shang¹, Fengwei Li¹, Runqiang Yang², Chunyin Zhang³, Yangjie Lu⁴ and Xiaohong Yu^1,

¹School of Marine and Bioengineering, Yan Cheng Institute of Technology, Yancheng 224051, China

²College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China

³Yancheng Green Garden Saline Soil Agriculture Technology Co., Ltd., Yancheng 224001, China

⁴Yancheng Huijia Aquatic Products Technology Co., Ltd., Yancheng 224300, China

Cite this paper:
Dujun Wang, Jie Wang, Jingying Zheng, Yueling Shang, Fengwei Li, Runqiang Yang, Chunyin Zhang, Yangjie Lu and Xiaohong Yu. Ca²⁺ and ABA on the Accumulation of GABA and Flavonoids in Germinated Salicornia bigelovii Torr. under NaCl Stress. Journal of Food and Nutrition Research. 2021; 9(5):263-273. doi: 10.12691/jfnr-9-5-4

Abstract

The effects of CaCl₂ and abscisic acid (ABA) on the sprouts growth, γ-aminobutyric acid (GABA) and flavonoids accumulation in germinated Salicornia bigelovii Torr. were investigated under NaCl stress. Results showed that the content of GABA and flavonoids increased by 1.20 and 1.19 times under 5 mM CaCl₂ treatment compared with the control group. The key enzymes including glutamate decarboxylase (GAD) and diamine oxidase (DAO), phenylalanine ammonolyses (PAL), cinnamicacid-4-hydroxylase (C4H) and 4-coumarate: CoA ligase (4CL) were increased significantly (p<0.05). After being treated with 50 μM ABA under 7.5 g•L^-1 NaCl, S. bigelovii had the higher content of GABA (398.26 mg•100g^-1) and flavonoids (36.90 mg•g^-1) than control group (p<0.05). The mRNA levels of key enzymes were consistent with GABA and flavonoids accumulation. Our results provided the basic for further studies key enzyme genes affect the GABA and flavonoids accumulation and laid the foundation for high value utilization of S. bigelovii.

Keywords:
Salicornia bigelovii Torr combined stress γ-aminobutyric acid flavonoids key enzyme gene

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