Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: Editor-in-chief: Prabhat Kumar Mandal
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Journal of Food and Nutrition Research. 2019, 7(10), 688-695
DOI: 10.12691/jfnr-7-10-1
Open AccessArticle

Lotus Leaf Ethanol Extract and Nuciferine Suppress Adipocyte Differentiation by Regulating Akt-mTORC1 Signaling in 3T3-L1 Cells

Ahyoung Yoo1, Young Jin Jang1, Jiyun Ahn1, Chang Hwa Jung1, Won Hee Choi2 and Tae Youl Ha1,

1Division of Food Functionality Research, Korea Food Research Institute, Wanju-gun, 55365, Republic of Korea

2Research Center, Teazen, Anyang-si, 14067, Republic of Korea

Pub. Date: October 02, 2019

Cite this paper:
Ahyoung Yoo, Young Jin Jang, Jiyun Ahn, Chang Hwa Jung, Won Hee Choi and Tae Youl Ha. Lotus Leaf Ethanol Extract and Nuciferine Suppress Adipocyte Differentiation by Regulating Akt-mTORC1 Signaling in 3T3-L1 Cells. Journal of Food and Nutrition Research. 2019; 7(10):688-695. doi: 10.12691/jfnr-7-10-1


Lotus leaf has been reported to exert anti-inflammatory, hypolipidemic, and hepatoprotective effects. However, the effect of lotus leaf on adipocyte differentiation and its action mechanism have not been clarified. In this study, 3T3-L1 preadipocytes were incubated with or without lotus leaf ethanol extract (EEN) for 8 days. Microscopic inspection and Oil Red O staining indicated that EEN treatment significantly reduced adipogenesis in 3T3-L1 cells. EEN also downregulated the protein levels of adipogenic transcription factors including sterol regulatory element binding protein 1 (SREBP1), peroxisome proliferator-activated receptor-gamma (PPARγ), and CCAAT/enhancer binding protein α (C/EBPα), and target genes such as adipocyte binding protein 2 (aP2) and fatty acid synthase (FAS) in a dose-dependent manner. In order to understand whether nuciferine, the primary active component of EEN contributed to the anti-adipogenic activity of EEN, we examined the effect of nuciferine on adipogenesis related gene expression. Nuciferine significantly reduced expression of adipogenic transcription factors and target genes. Notably, nuciferine downregulated the phosphorylation of Akt, mammalian target of rapamycin complex 1 (mTORC1), S6K, and 4EBP1. These results suggest that lotus leaf ethanol extract exerts anti-adipogenic activity, and could be partially mediated through the regulation of the Akt-mTORC1 signaling pathway by nuciferine.

lotus leaf nuciferine adipogenesis Akt-mTORC1 signaling 3T3-L1 cell

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