Purification and Analysis of Norcadia spp .Azoreductase

M. Shah

Journal of Applied & Environmental Microbiology. 2014, 2(5), 237-243
DOI: 10.12691/jaem-2-5-6

Open AccessArticle

Purification and Analysis of Norcadia spp .Azoreductase

¹Industrial Waste Water Research Laboratory Division of Applied & Environmental Microbiology Enviro Technology Limited Gujarat, India

Pub. Date: August 01, 2014

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Cite this paper:
M. Shah. Purification and Analysis of Norcadia spp .Azoreductase. Journal of Applied & Environmental Microbiology. 2014; 2(5):237-243. doi: 10.12691/jaem-2-5-6

Abstract

Nocardia spp. was isolated as Acid Red degrader. The reductive cleavage of azo bond was catalyzed by azo reductase, the key enzyme for the azo dye degradation. Azo reductase from the Nocardia spp. isolate was purified and characterized. Azoreductase was found to have the following features. The catalytic reduction of acid red 37 by purified azoreductase in the presence of NADH as electron donor was studied and the products of degradation were determined as 1-{3-amino-5-[(aminoxy)sulfonyl]phenyl}ethanol and 7,8-diamino-3[(aminoxy)sulfonyl]naphthalene-1-ol.

Keywords:
Nocardia Acid Red LC-MS NADH Azoreductase

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