Article citationsMore >>

Ryan A, Wang C, Laurieri N, Westwood I, Sim E (2010) Reaction mechanism of azoreductases suggests convergent evolution with quinone oxidoreductases. Protein Cell 1 (8): 780-790.

has been cited by the following article:

Article

Purification and Analysis of Norcadia spp .Azoreductase

1Industrial Waste Water Research Laboratory Division of Applied & Environmental Microbiology Enviro Technology Limited Gujarat, India


Journal of Applied & Environmental Microbiology. 2014, Vol. 2 No. 5, 237-243
DOI: 10.12691/jaem-2-5-6
Copyright © 2014 Science and Education Publishing

Cite this paper:
M. Shah. Purification and Analysis of Norcadia spp .Azoreductase. Journal of Applied & Environmental Microbiology. 2014; 2(5):237-243. doi: 10.12691/jaem-2-5-6.

Correspondence to: M.  Shah, Industrial Waste Water Research Laboratory Division of Applied & Environmental Microbiology Enviro Technology Limited Gujarat, India. Email: shahmp@uniphos.com

Abstract

Nocardia spp. was isolated as Acid Red degrader. The reductive cleavage of azo bond was catalyzed by azo reductase, the key enzyme for the azo dye degradation. Azo reductase from the Nocardia spp. isolate was purified and characterized. Azoreductase was found to have the following features. The catalytic reduction of acid red 37 by purified azoreductase in the presence of NADH as electron donor was studied and the products of degradation were determined as 1-{3-amino-5-[(aminoxy)sulfonyl]phenyl}ethanol and 7,8-diamino-3[(aminoxy)sulfonyl]naphthalene-1-ol.

Keywords