Killer Yeasts Isolated from Ivorian Cocoa Fermentation and their Antagonist Activity on Phytopathogens Dickeya solani and Dickeya dadantii

SAMAGACI Lamine^1, , OUATTARA Hadja¹, KOSSONOU Silver¹, KADET Victoria¹, LEMAIRE Marc² and NIAMKE Sebastien¹

¹Laboratory of Biotechnology, Agriculture and Valorization of Biological Resources, Felix Houphouët –Boigny University, P.O. Box 582 Abidjan 22, Ivory Coast Abidjan, Côte d’Ivoire

²Laboratoire Microorganism, Adaptation and Pathogenesis, Claude Bernard University, Lyon, France

Cite this paper:
SAMAGACI Lamine, OUATTARA Hadja, KOSSONOU Silver, KADET Victoria, LEMAIRE Marc and NIAMKE Sebastien. Killer Yeasts Isolated from Ivorian Cocoa Fermentation and their Antagonist Activity on Phytopathogens Dickeya solani and Dickeya dadantii. American Journal of Food Science and Technology. 2024; 12(6):207-212. doi: 10.12691/ajfst-12-6-3

Abstract

The yeasts involved in cocoa fermentation play an important role in obtaining good cocoa beans’ quality. Apart from cocoa fermentation, these yeasts could have a good inhibitory action against the growth of any phytopathogenic microorganism notably Dickeya dqdantii and Dickeya solani. This study is the first to evaluate the inhibitory action of yeasts involved in cocoa fermentation on the rot potato phytopathogen. For this purpose, the yeasts isolated from Côte d’Ivoire were confronted with the sensitive strain of Saccharomyces cerevisiae (W303). Moreover, the yeast killers were used to evaluate their inhibitory action on Dickeya solani and Dickeya dadantii. Finally, the physicochemical parameters (temperature, pH and salt concentration) capable to influence the inhibitory action of killer yeasts on Dickeya solani and Dickeya were evaluated. Thirty-three (33) yeasts out of the yeasts isolates tested were capable to produce toxic substances against the sensitive strain of Saccharomyces cerevisiae (W303). Among these yeasts, 14 are capable to inhibit the growth of Dickeya solani at temperature of 25 °C and 30 °C as well as pH of 4.5 and 6. Four (4) of these yeasts (C1P8, E10P10, D12P12 and E10P19) exert an inhibiting action on the growth of Dickeya dadantii in the same condition as before. These four yeasts isolates can be used in the biological fight against these two phytopathogens responsible for potato rot.

Keywords:
Cocoa yeast Dickeya dadantii Dickeya solani inhibition activity

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References:

[1]	Freimoser F.M., Rueda-Mejia M.P., Tilocca B., Migheli Q., "Biocontrol yeasts: Mechanisms and applications", World Journal of Microbiology and Biotechnology, 35, 1-19, 2019.
[2]	Yehia H.M., El-Khadragy M.F., Al-Masoud A.H., Ramadan E.M., El-Din M.F.S., "Killer yeast isolated from some foods and its biological activity", Food Science and Technology, 42, e119721, 2022.
[3]	Díaz M.A., Pereyra M.M., Picón-Montenegro E., Meinhardt F., Dib J.R., "Killer yeasts for the biological control of postharvest fungal crop diseases", Microorganisms, 8 (11), 1680, 2020.
[4]	Koffi O., Samagaci L., Goualie B., Niamke S., "Diversity of yeasts involved in cocoa fermentation of six major cocoa-producing regions in Ivory Coast", Eur Sci J, 13, 496-516, 2017.
[5]	Samagaci L., Ouattara H.G., Goualié B.G., Niamke S.L., "Polyphasic analysis of pectinolytic and stress-resistant yeast strains isolated from Ivorian cocoa fermentation", Journal of Food Research, 4 (1), 124, 2015.
[6]	Ruggirello M., Nucera D., Cannoni M., Peraino A., Rosso F., Fontana M., et al., "Antifungal activity of yeasts and lactic acid bacteria isolated from cocoa bean fermentations", Food Research International, 115, 519-25, 2019.
[7]	Laurila J., Ahola V., Lehtinen A., Joutsjoki T., Hannukkala A., Rahkonen A., et al., "Characterization of Dickeya strains isolated from potato and river water samples in Finland", European Journal of Plant Pathology, 122, 213-25, 2008.
[8]	van der Wolf J.M., Cahill G., Van Gijsegem F., Helias V., Humphris S., Li X., et al. Isolation, Detection and Characterization of Pectobacterium and Dickeya Species. In: Van Gijsegem F, van der Wolf JM, Toth IK, editors. Plant Diseases Caused by Dickeya and Pectobacterium Species. Cham: Springer International Publishing; 2021. p. 149-73.
[9]	Potrykus M., Golanowska M., Sledz W., Zoledowska S., Motyka A., Kolodziejska A., et al., "Biodiversity of Dickeya spp. isolated from potato plants and water sources in temperate climate", Plant Disease, 100 (2), 408-17, 2016.
[10]	Woods D.R., Bevan E.A., "Studies on the Nature of the Killer Factor produced by Saccharomyces cerevisiae", Journal of General Microbiology, 51, 115-26., 1968.
[11]	Salek A., Schnettler R., Zimmermann U., "Transmission of killer activity into laboratory and industrial strains of Saccharomyces cerevisiae by electroinjection", FEMS Microbiology Letters, 70 (1), 67-72, 1990.
[12]	Gulbiniene G., Kondratiene L., Jokantaite T., Serviene E., Melvydas V., Petkuniene G., "Occurrence of killer yeast strains in fruit and berry wine yeast populations", Food Technology and Biotechnology, 42 (3), 159-63, 2004.
[13]	Baeza M.E., Sanhueza M.A., Cifuentes V.H., "Occurrence of killer yeast strains in industrial and clinical yeast isolates", Biological Research, 41 (2), 173-82, 2008.
[14]	Tansel Y.H., Cengiz Ç., "Isolation and Characterization of Amylase Producing Yeasts and Improvement of Amylase Production", Turkish Journal of Biochemistry/Turk Biyokimya Dergisi, 38 (1), 101–8., 2013.
[15]	Clarke P., Steel K., "Rapid and simple biochemical tests for bacterial identification", Identification method for microbiologists Part A Academic Press, London, 111-5, 1966.
[16]	de Lima J.R., Gonçalves L.R.B., Brandão L.R., Rosa C.A., Viana F.M.P., "Isolation, identification, and activity in vitro of killer yeasts against Colletotrichum gloeosporioides isolated from tropical fruits", Journal of basic microbiology, 53 (7), 590-9, 2013.
[17]	Meersman E., Steensels J., Paulus T., Struyf N., Saels V., Mathawan M., et al., "Breeding strategy to generate robust yeast starter cultures for cocoa pulp fermentations", Applied and Environmental Microbiology, 81 (18), 6166-76, 2015.
[18]	Bouatenin K.M.J.-P., Kouame K.A., Djeni N.d.T., Koffi N.g.G., Dje K.M., "Production of Attieke by the Technique of Drying of Cassava Ferment", Journal of Food Quality, 2021, 6697835, 2021.
[19]	Balakrishnan D., Kumar S.S., Sugathan S. Amylases for Food Applications—Updated Information. In: Parameswaran B, Varjani S, Raveendran S, editors. Green Bio-processes: Enzymes in Industrial Food Processing. Singapore: Springer Singapore; 2019. p. 199-227.
[20]	Anoop Kumar V., Suresh Chandra Kurup R., Snishamol C., Nagendra Prabhu G. Role of Cellulases in Food, Feed, and Beverage Industries. In: Parameswaran B, Varjani S, Raveendran S, editors. Green Bio-processes: Enzymes in Industrial Food Processing. Singapore: Springer Singapore; 2019. p. 323-43.
[21]	Pretscher J., Fischkal T., Branscheidt S., Jäger L., Kahl S., Schlander M., et al., "Yeasts from Different Habitats and Their Potential as Biocontrol Agents", Fermentation, 4 (2), 31, 2018.
[22]	Buzzini P., Corazzi L., Turchetti B., Buratta M., Martini A., "Characterization of the in vitro antimycotic activity of a novel killer protein from Williopsis saturnus DBVPG 4561 against emerging pathogenic yeasts", FEMS microbiology letters, 238 (2), 359-65, 2004.
[23]	Sainz J., Pizarro F., Pérez‐Correa J.R., Agosin E., "Modeling of yeast metabolism and process dynamics in batch fermentation", Biotechnology and bioengineering, 81 (7), 818-28, 2003.
[24]	Mayer M., Quinlan S., "The Effects of Salt Concentration on Bacterial Growth. Available: https://sciencing.com/effects-salt-concentration-bacterial-growth-5924409.html", 2018.
[25]	Droby S., Wisniewski M., Teixidó N., Spadaro D., Jijakli M.H., "The science, development, and commercialization of postharvest biocontrol products", Postharvest Biology and Technology, 122, 22-9, 2016.