American Journal of Food Science and Technology
ISSN (Print): 2333-4827 ISSN (Online): 2333-4835 Website: Editor-in-chief: Hyo Choi
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American Journal of Food Science and Technology. 2020, 8(2), 49-55
DOI: 10.12691/ajfst-8-2-2
Open AccessArticle

Preparation of New Carbonated Beverages Based on Hydrolyzed Whey by Fruit and Some Herbs

Atallah A. Atallah1, and Dalia G. Gemiel1

1Department of Dairy Science, Faculty of Agriculture, Benha University, Egypt

Pub. Date: March 02, 2020

Cite this paper:
Atallah A. Atallah and Dalia G. Gemiel. Preparation of New Carbonated Beverages Based on Hydrolyzed Whey by Fruit and Some Herbs. American Journal of Food Science and Technology. 2020; 8(2):49-55. doi: 10.12691/ajfst-8-2-2


In an attempt to develop new carbonated beverages, whey was hydrolyzed by lactase enzyme at pH 6.6. Whey beverages were prepared by blending sugar (10%), fruit juice or/and herbs extract to hydrolyzed whey. The hydrolyzed whey beverages were carbonated by injecting CO2 at a pressure of 15 psi/15sec. The carbonated whey beverages were analyzed for chemical, physical, microbiological and evaluated for its sensory properties. The TS, ash, total carbohydrates, and pH values had recorded significant (P < 0.05) between all treatments in carbonated beverages. But the protein and fat contents showed no significant between all treatments. From the results, it is clear that T8 treatment recorded the significantly high value of viscosity compared to T3 treatment which had the lowest values. Energy value of carbonated whey beverages ranged from 56.85 to 61.21 kcal 100g-1 sample. The total bacterial counts were ranged from 0.95 to 1.04 log10 CFU mL-1 in all treatments. The acceptability value in carbonated whey beverages had an increasing trend with a treatment containing green tea, lemon and/or peppermint. Higher acceptability values were obtained for the T8 sample and minimum acceptability scores were attained for the T1 sample of carbonated whey beverages.

hydrolyzed whey carbonated whey beverages chemical analysis Energy value sensory properties physical analysis

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[1]  Deepa, C. K. and Krishnaprabha, V, “Development and nutrient, antioxidant and microbial analysis of muskmelon and whey water and probiotic incorporated squash”. Nt. J. Curr. Microbiol. App. Sci, 3, 267-271, 2014.
[2]  Siso, M. I. G, “The biotechnological utilization of cheese whey: A review”. Bioresource Tech, 57, 1-11, 1996.
[3]  Jandal, J. M, “Dairy beverages”. Beverages and Food World, 11, 30-32, 1996.
[4]  Gupta, V. K, “Overview of processing and utilization of dairy by-products”. Indian Dairyman, 52(5), 55-60, 2000.
[5]  Serafini, M., Ghiselli, A. and Ferro-Luzzi, A, “In vivo antioxidant effect of green and black tea in man”. Eur. J. Clin. Nutr, 50, 28-32, 1996.
[6]  Hollman, P. C., van het Hof, K. H., Tijburg, L. B. and Katan, M. B, “Addition of milk does not affect the absorption of flavonols from tea in man”. Free Radic. Res, 34, 297-300, 2000.
[7]  González, M. E., Moreno, D. A. and Garcia-Viguera, C, “Genotype and harvest time influence the phytochemical quality”. Food Chemistry, 56, 1669-1675, 2008.
[8]  Farag, R. S., El Baroty, G. S. and Basuny, A. M, “Safety evaluation of olive phenolic compounds as natural antioxidants”. International Journal of Food Sciences and Nutrition, 54(3), 159-175, 2003.
[9]  AOAC “Official Methods of Analysis of Association of Official Analytical Chemists 19th ed., Published by AOAC International”, Gaithersburg, Maryland, USA, 2012.
[10]  BSI “Dried milk”: determination of titratable acidity and pH values (Reference method) ISO, 6091, 2010.
[11]  Prentice, G. A. and Langridge, E. W, “Laboratory control in milk product manufacture”: Technology of dairy products. Early R. (Ed). Blackie and Sons Ltd, London, 247-269, 1992.
[12]  Viturawong, Y., Achayuthakan, P. and Suphantharika, M, “Gelatinization and rheological properties of rice starch/ xanthan mixtures”: Effects of molecular weight of xanthan and different salts. Food Chemistry, 111(1), 106-114, 2008.
[13]  Dilipkumar, K. S. and Yashi, S, “Preparation and quality characteristics of carbonated beverage prepared by profiteered, ultrafiltered acidic whey with different fruit juices”. Inter. J. Innov. Technol. & Sci, 2(3), 52-60, 2014.
[14]  Ranganna, S, “Handbook of analysis and quality control for fruits and vegetable products”. Tata Mc. Graw Hill Publishing Company Limited. New Delhi: 594-645, 1994.
[15]  Mansour, E. H. and Khalil, A. H, “Characteristics of low-fat beef burger as influenced by various types of wheat fibers”. Food Res. Inter, 30, 199-205, 1997.
[16]  IDF “Milk and milk products”: Enumeration of Microorganisms' colony count technique at 30°C. IDF 100 B, 1991.
[17]  Difco “Dehydrated culture media and reagents for microbiological and clinical laboratory procedures”, pub-Difco lab. Detroit's Michigan, USA, 1984.
[18]  Sattin, E., Andreani, N. A., Carraro, L., Lucchini, R., Fasolato, L., Telatin, A., Balzan, S., Novelli, E., Simionati, B. and Cardazzo, B, “A Multi-Omics approach to evaluate the quality of milk whey used in Ricotta cheese production”. Frontiers in Microbiology, 7, 1-13, 2016.
[19]  APHA “Standard methods for the examination of dairy products”. American Publ. Health Assoc. Inc. 16th Ed., Washington D.C., 1992.
[20]  IDF “Milk and milk products”: Enumeration of Yeasts and Moulds (Colony count technique at ). IDF: 94B, 1990.
[21]  Clarke, G. M. and Kempson, R. E, “Introduction to the Design and Analysis of Experiments”. Arnold, a Member of the Holder Headline Group, 1st Eds., London, UK, 1997.
[22]  Suresh, K. B. and Jayaprakasha, H. M, “Process optimization for preparation of beverage from lactose hydrolyzed whey permeate”. J. Food Sci. Technol, 41, 27-32, 2004.
[23]  Rizk, A. E, “Study of production functional beverages of milk permeate fortified with fruit and herbs. Middle East Journal of Applied Sciences, 06(01), 155-161, 2016.
[24]  Singh, S., Khemariya, P. and Rai, A, “Process optimization for the manufacture of lemon based beverage from hydrolyzed whey”. J. Food Sci. Technol, 51(4), 691-699, 2014.
[25]  Silva e Alves, A. T., Spadoti, L. M., Zacarchenco, P. B. and Trento, F. K, “Probiotic functional carbonated whey beverages: Development and quality evaluation”. Beverages, 4, 49, 2018.
[26]  Patel, R, “Technology for carbonated lemon whey beverages”. Research & Reviews: Journal of Food and Dairy Technology, 5(2), 30-37, 2017.
[27]  Sameen, A., Tariq, R. M., Huma, N. and Khan, M. I, “Effect of stabilizers on the quality of carbonated flavoured whey drink”. African Journal of Agricultural Research, 8(5), 445-448, 2013.