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ISSN (Print): 2374-1155 ISSN (Online): 2374-1163 Website: https://www.sciepub.com/journal/ajfn Editor-in-chief: Mihalis Panagiotidis
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American Journal of Food and Nutrition. 2024, 12(1), 1-15
DOI: 10.12691/ajfn-12-1-1
Open AccessArticle

Effect of Silybum marianum Seeds Extract Intervention on Biochemical Parameters, Histological Changes, and Apoptosis and Cell Cycle of Liver Tissue in Benzo[a]pyrene Injected Rats

Yousif A. Elhassaneen1, , Tarek A. Afifi1, Mona A. Elhefny1 and Asmaa I. Bayomi2

1Department of Nutrition and Food Science, Faculty of Home Economics, Menoufia University, Shebin El-Kom, Egypt

2Department of Zoology, Faculty of Science, Menoufia University, Shebin El-Kom, Egypt

Pub. Date: March 12, 2024
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Cite this paper:
Yousif A. Elhassaneen, Tarek A. Afifi, Mona A. Elhefny and Asmaa I. Bayomi. Effect of Silybum marianum Seeds Extract Intervention on Biochemical Parameters, Histological Changes, and Apoptosis and Cell Cycle of Liver Tissue in Benzo[a]pyrene Injected Rats. American Journal of Food and Nutrition. 2024; 12(1):1-15. doi: 10.12691/ajfn-12-1-1

Abstract

The present study aims to investigate the effect of Silybum marianum seeds extract intervention on biochemical parameters, histological changes, and apoptosis and cell cycle of liver tissue in Benzo[a]pyrene injected rats. Rats (n=36), were randomly assigned to six groups of 6 rats per each. Group 1 served as normal control. Groups 2 to 6 were injected intraperitoneally with B[a]P for 14 days and group (2) acted as a model control, while groups (3-6) received SME at concentrations of 200, 400, 600, and 800 mg/kg bw/d by oral gavage for 28 days each, respectively. Treatment of rats with B[a]P caused a significant increased (p≤0.05) in liver enzymes functions (AST, 126.07%, ALT, 120.24%, and ALP, 142.32%), compared to normal control rats. Also, B[a]P treatment brought a significant (p≤0.05) decrease in serum triglycerides (TG's), high density lipoprotein (HDL), and liver glycogen, albumin and glutathione (GSH) content by the ratio of -78.48, -39.98, -71.40, -43.03 and - 35.29%, respectively. Furthermore, B[a]P treatment brought a significant (p≤0.05) increase in serum cholesterol and liver malonaldehyde (MDA), the biomarkers of oxidative stress in cells, content by the ratio of 37.47 and 225.34%, respectively. Additionally, adverse molecular (apoptosis and cell cycle) and histopathological changes of liver tissue as the result of B[a]P treatment. Supplementation of the rat diets with SME (200 to 800 mg/kg bw/d) prevented the rise of liver function enzymes activities, MDA liver content and serum cholesterol as well as increases in serum TG's and albumin and the liver glycogen and GSH content. Also, improvement in both molecular and histological parameters was recorded. The rate of improving in all of these parameters exhibited a dose-dependent increase with the SME intervention. The results of this study suggest that treatment with SME in the tested concentrations proved beneficial on manipulation of the liver biochemical, molecular and histological injuries induced by B[a]P.

Keywords:
liver functions glycogen triglycerides albumin glutathione malonaldehyde flow cytometer

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References:

[1]  Crawford, J. M. (1999). The Liver and the Biliary Tract., Pathologic Basis of Disease. Eds. R. S. Cotran, V. Kumar, and T. Collins. W. B. Saunders Company: Philadelphia, USA.
 
[2]  Voet, D. & Voet, G. (1990). Biochemistry, 1st Edition, John Wiley and Sons, USA.
 
[3]  Kebamo, S., Shibiru T. & Bekesho, G. (2015). The Role of Biotransformation in Drug Discovery and Development, J Drug MetabToxicol,5: 1-13.
 
[4]  Elhassaneen, Y. A. (1996). Biochemical and technological studies on pollution of fish with pesticides and polycyclic aromatic hydrocarbons. Ph.D. Thesis., Faculty of Agriculture, Mansoura University, Egypt.
 
[5]  Elhassaneen, Y. (2004). The effect of charcoal broiled meat consumption on antioxidant defense system of erythrocytes and antioxidant vitamins in plasma. Nutrition Research, 24 (6): 435 - 446.
 
[6]  Elhassaneen, Y., Ragab, R. & Mashal, R. (2016). Improvement of bioactive compounds content and antioxidant properties in crackers with the incorporation of prickly pear and potato peels powder. International Journal of Nutrition and Food Sciences, 5(1): 53-61.
 
[7]  Anwar, W., Khaled Hussein M., Amra Hassan A., El-Nezami Hani. & Loffred Christopher A. (2008) Changing pattern of hepatocellular carcinoma (HCC) and its risk factors in Egypt: possibilities for prevention. Mutat Res, 659:176–84.
 
[8]  Gray, J. I. & Morton, D. I. (1981): Some toxic compounds ‎produced in food by cooking and processing: A review. J. Human ‎Nutr, 35: 5–23.‎
 
[9]  Elhassaneen, A. & Tawfik, L. (1998). The presence of some carcinogens in human foods distributed in Egyptian local markets. Journal of Home Economics, 8 (3): 23 - 38.
 
[10]  Elhassaneen, Y. A. (1999). "Toxicological and biochemical effects of polycyclic aromatic hydrocarbon compounds produced in fish by cooking and processing”. 6 th Arabic Conference on Food Science and Technology, 16 – 18 th march, Egyptian Society of Food Science and Technology, Cairo, Egypt, pp. 249 – 270.
 
[11]  Elhassaneen, Y. (2002): New and quickly biological method for detection the potential chemical toxins and/or carcinogens in foods. Proceedings of2nd scientific Conference on Foodborne Contamination and Egyptian’s Health (24–24 April), Faculty of Agriculture, Mansoura University, Mansoura, Egypt 371-394.
 
[12]  Huosein, M. (2011). The effect of phytochemicals on toxic and/or carcinogenic substances formed during cooking and processing of meat " Ph.D. Thesis in Nutrition and Food Science, Faculty of Home Economics, Minoufiya University, Egypt.
 
[13]  Adeyeye, S. O. (2020). Polycyclic Aromatic Hydrocarbons in Foods: A Critical Review. Current Nutrition & Food Science. 16 (6): 866 – 873.
 
[14]  Emerole, G. O., Uwaifo, A. O. & Bababunmi, E. A. (1982): The presence of aflatoxine and some polycyclic aromatic hydrocarbons in human foods. Cancer Lett 15: 123-129.
 
[15]  Larsson, B. K., Sahllerg, G. P., Eriksson, A. T. & Busk, L. A. (1983): Polycyclic aromatic hsdrocarbons in grilled food. J. Agric Fd Chem, 31:867-873.
 
[16]  Maanen van, J. M. S., Moonen, E. J. C., Maas, L. M., Kleinjans, J. C. S. & Schooten van, F. j. (1994): Formation of aromatic DNA adducts in white blood cells in relation to urinary excretion of 1-hydroxypyrene during consumption of grilled meat. Carcinogenesis, 15: 2263-2268.
 
[17]  Bassiouny, E. (1999). The effect of grilled meat consumption on health. MSc Thesis in Nutrition and Food Science, Faculty of Home Economics, Minoufiya University, Shebin El-Kom, Egypt.
 
[18]  Hassan, A. A. (2005). The effect of antioxidants on the formation of toxic and carcinogenic substances in some smoked food. M Sc Thesis Fac Of Home Economics, Minufyia Univ Egypt.
 
[19]  Elhassaneen, Y. & El-Badawy, A. (2013). Influence of Charcoal Broiled Meat Consumption on the Liver Functions and Non-Enzymatic Antioxidants in Human Blood. Food and Nutrition Sciences, 4 (1): 90 – 99.
 
[20]  U.S. Environmental Protection Agency. (2005): Supplemental Guidance for Assessing Cancer Susceptibility from Early-Life Exposure to Carcinogens. Available from.
 
[21]  Sims, P. & Grover, P. L. (1974). Epoxides in polycyclic aromatic hydrocarbon metabolism and carcinogenesis. Advances in cancer research, 20:165-274.‏
 
[22]  Harvey, R. G. (1985). Polycyclic hydrocarbons and carcinogenesis. ACS Symp. Ser. 283, American Chemical Society, Washington, D.C.
 
[23]  Plakunov, I., Smolarek, T. A., Fischer, L. D., Wiley, J.C. & Baird, W.M. (1987): Separation by ion-pair high-performance liquid chromatography of the glucuronid, sulfate and glutathione conjugates formed from benzo(a)pyrene in cell cultures rodents, fish and humans. Carcinogenesis, 8: 59-66.
 
[24]  Hawkins, E. W., Walker, W. W., Overstreet, R. M., Lytle, T. F. & Lytle, J. S. (1990). Carcinogenic effects of some polycyclic aromatic hydrocarbons on the Japanese medaka and Guppy in waterborne exposures. The Science of the Total Environment, 94: 155-167.
 
[25]  Elhassaneen, Y. A., Khater, O. M. & Morsey, A. A. (1997). The effect of vitamins on the cancer initiation induced by some food chemical carcinogenic pollutants in vitro. The Second Conference: The Role of Women and Egyptian Associations in Environment Protection and Community Development (25-26 August), Dept. of Home Economics, Faculty of Agriculture, Alexandria University, Egypt, 252-282.
 
[26]  Mehram, E. & Sayed Ahmed, S. (2020). Benzo(a)pyrene induced liver disorders in rats: possible protective effects of mulberry (Morus alba L.) leaves. Research Journal in Specific Education, 6 (8): 769-798.
 
[27]  Mahran, M. Z. & Elhassaneen, Y. A. (2023). A Study of the Physical, Chemical, Phytochemical and Nutritional Properties of Wild Silybum marianum L. Seeds Oil to Investigate Its Potential Use to Boost Edible Oil Self-Sufficiency in Egypt. Alexandria Science Exchange Journal, 44, (1): 81-91.
 
[28]  Hawkins, E. W., Walker, W. W., Overstreet, R. M., Lytle, T. F. & Lytle, J. S. (1988). Dose-related carcinogenic effect of water- borne benzo(a) pyrene on livers of two small fish species. Ecotoxicology and environmental safety, 16: 219-231.
 
[29]  Elhassaneen, Y., Ragab, S. & Badawy, R. (2016). Antioxidant activity of methanol extracts from various plant parts and their potential roles in protecting the liver disorders induced by benzo(a)pyrene. Public Health International, 2 (1): 38-50.
 
[30]  Anita, L. (2018). Polycyclic Aromatic Hydrocarbons: Sources, Importance and Fate in the Atmospheric Environment. Current Organic Chemistry, 22 (11): 1050 – 1069.
 
[31]  Weinstein, N. D. (1978). Individual differences in reactions to noise: a longitudinal study in a college dormitory. Journal of applied psychology, 63(4): 458.‏
 
[32]  Philips, D. & Sims, P. (1979). PAH metabolites: their reaction with nucleic acids. Chemical carcinogens and DNA, 2: 9-57.
 
[33]  Harvey, J. (1982). θ-S relationships and water masses in the eastern North Atlantic. Deep Sea Research Part A. Oceanographic Research Papers, 29(8): 1021-1033.‏
 
[34]  Mackenzie, K. M. & Murray Angevine, D. (1981). Infertility in mice exposed in utero to benzo (a) pyrene. Biology of reproduction, 24(1): 183-191.‏
 
[35]  El-Safty, A. (2012). Production of some important nutritional and functional compounds from the by-products of food processing companies. Ph.D. Thesis in Nutrition and Food Science, Faculty of Home Economics, Minoufiya University, Egypt.
 
[36]  Elhassaneen, Y. & Sayed, R. (2015). Polycyclic aromatic hydrocarbons formation in charcoal broiled meatballs are reduced by addition of onion peel extracts to ground beef. Proceeding of 2nd International Conference on Food and Biosystems Engineering (2nd I.C. FABE2015), 28-31 May, 2015), [95-104], Mykonos, Greece .
 
[37]  Fayez, S. (2016). The effect of turmeric and curcumin on liver cancer induced by benzo[a]pyrene in rats. M.Sc. Thesis in Home Economics (Nutrition and Food Science), Faculty of Specific Education, Port Said University, Egypt.
 
[38]  Elmaadawy, A.(2016). Phyto-extracts applied in beef meatballs ameliorates hyperglycemia and its complications in alloxan-induced diabetic rats. Journal of Home Economics, 26(3), 1-16.‏
 
[39]  Elhassaneen, Y., Badran.H., Abd EL-Rahman. A. & Badawy, N. (2021). Potential Effect of Milk Thistle on Liver Disorders Induced by Carbon Tetrachloride. Journal of Home Economics, 31 (1): 83-93.
 
[40]  Abenavoli, L., Capasso, R., Milic, N. & Capasso, F. (2010). Milk thistle in liver diseases: past, present, future. 24(10):1423-1432.
 
[41]  Bijak, M. (2017). Silybin, a Major Bioactive Component of Milk Thistle (Silybum marianum L. Gaernt.)-Chemistry, Bioavailability, and Metabolism. Molecules. 10; 22(11):1942.
 
[42]  Flora, K., Hahn, M., Rosen, H. & Benner, K. (1998). Milk thistle (Silybum marianum) for the therapy of liver disease. The American Journal of Gastroenterology, 93, 139–143.
 
[43]  Abenavoli, L., Izzo, A. A., Milić, N., Cicala, C., Santini, A. & Capasso, R. (2018). Milk thistle (Silybum marianum): A concise overview on its chemistry, pharmacological, and nutraceutical uses in liver diseases. Phytotherapy Research, 32: 2202–2213.
 
[44]  Andrew, R. & Izzo, A. A.(2017).Principles of pharmacological research of nutraceuticals. British Journal of Pharmacology, 17: 1177– 1194.
 
[45]  Kvasnička, F., Bıba, B., Ševčı́k, R., Voldřich, M. & Kratka, J. (2003). Analysis of the active components of silymarin. Journal of Chromatography A, 990(1-2): 239-245.‏
 
[46]  Kroll, D. J., Shaw, H.S. & Oberlies, N. H. (2007). "Milk Thistle Nomenclature: Why It Matters in Cancer Research and Pharmacokinetic Studies". Integrative Cancer Therapies, 6 (2): 110–9.
 
[47]  Greenlee, H., Abascal, K., Yarnell, E. & Ladas, E. (2007). "Clinical Applications of Silybum marianum in Oncology". Integrative Cancer Therapies. 6 (2): 158–65.
 
[48]  Soto, C., Raya, L., Juárez, J., Pérez, J. & González, I. (2014). Effect of Silymarin in Pdx-1 expression and the proliferation of pancreatic β-cells in a pancreatectomy model. Phytomedicine, 21: 233– 239.
 
[49]  Poruba, M., Matušková, Z., Kazdová, L., Oliyarnyk, O., Malínská, H., Tozzi di Angelo, I. & Vecera, R. (2016). Positive effects of different drug forms of silybin in the treatment of the metabolic syndrome. Physiological Research, 64:S507– S512.
 
[50]  Sayin, F. K., Buyukbas, S., Basarali, M. K., Alp, H., Toy, H. & Ugurcu, V. (2016). Effects of Silybum marianum extract on high-fat diet-induced metabolic disorders in rats. Polish. J. Food Nutr Sci, 66: 43– 49.
 
[51]  Famouri, F., Salehi, M. M., Rostampour, N., Hashemi, E. & Shahsanaee, A. (2017). The effect of silymarin on the non-alcoholic fatty liver disease of children. J Herbmed Pharmacol, 6:16– 20.
 
[52]  Tajmohammadi, A., Bibi, M. & Hossein, H. (2018). Silybum marianum (milk thistle) and its main constituent, silymarin, as a potential therapeutic plant in metabolic syndrome: A review. Phytotheray research, 32 (10): 1933-1949.
 
[53]  Reeves, P., Nielsen, F. & Fahey, G. (1993). "AIN-93 Purified Diets for Laboratory Rodents: Final Report of the American Institute of Nutrition Ad Hoc Writing Committee on the Reformulation of the AIN-76A Rodent Diet". Journal of Nutrition, 123(11): 1939-1951.
 
[54]  Oludemi, T., Sandrina, A., Ricardo, C., Maria, J., Lillian, B., Ana, M., Gonz, A., Maria, F. & Isabel, C. (2017). The potential of Ganoderma lucidum extracts as bioactive ingredients in topical formulations, beyond its nutritional benefits. Food and Chemical Toxicology, 108: 139-147.
 
[55]  NRC, National Research Council (1996). Guide for the Care and Use of Laboratory Animals Washington: National Academy Press.
 
[56]  Shahid, A., Ali, R., Ali, N., Hasan, S. K., Bernwal, P., Afzal, S. M., Vafa, A. & Sultana, S. (2016). Modulatory effects of catechin hydrate against genotoxicity, oxidative stress, inflammation and apoptosis induced by benzo(a)pyrene in mice. Food and Chemical Toxicology, 92: 64–74.
 
[57]  Stroev, E. A. & Makarova, V. G. (1989). Laboratory Manual in Biochemistry, MIR Publishers, Moscow, USSR. ISBN: 5030007679.
 
[58]  Yound, D. S. (1975). Determination of GOT. Clin. Chem, 22 (5): 21-27.
 
[59]  Tietz, N.W. (1976). Fundamental of Clinical Chemistry. Philadelphia, W.B. Saunders, P. 243.
 
[60]  Vanessa, G., Nana, B., Maria, D., Belen, L., Sara, G. & Eloy, F. (2018). Overestimation of Albumin Measured by Bromocresol Green vs Bromocresol Purple Method: Influence of Acute-Phase Globulins. Laboratory Medicine, 49 (4): 355–361.
 
[61]  Shokri-Afra, H., Ostovar-Ravari, A. & Rasouli, M.(2016). Improvement of the classical assay method for liver glycogen fractions: ASG is the main and metabolic active fraction. Eur Rev Med Pharmacol Sci, 20:4328–36.
 
[62]  Fossati, P. & Prenape, L. (1982): Serum triglycerides deter-mined colorimeterically with enzyme that produce hydrogen peroxide. Clin. Chem, 28: 2077-2080.
 
[63]  Richmond, W. (1973). "Preparation and Properties of a Cholesterol Oxidase from Nocardia sp. and its Application to the Enzy-matic Assay of Total Cholesterol in Serum. Clinical Chemistry, 19:1350-1356.
 
[64]  Lopes-Virella, M. F., Stone, S., Ellis, S. and Collwell, J. A. (1977). Cholesterol determination in high-density lipoproteins separated by three different methods. Clin. Chem, 23(5): 882-886.
 
[65]  Ellman, G. L. (1959): Tissue sulphydryl groups. Archives of Biochemistry and Biophysics, 82: 70-77 .
 
[66]  Buege, J. A. & Aust, S. D. (1978): Microsomal lipid peroxidation in Packer L., (ed), Methods in enzymology, New York, NY, Academic, 52: 302 - 310.
 
[67]  Tribukait, B., Moberger, G. and Zetterberg, A. (1975): Methodological aspects for rapid flow cytofluorometry for DNA analysis of human urinary bladder cells. In: Haenen, C.; Hillen, H. and Wessels, S. eds. Pluse cytophotometry. Eureopean press Medicon. Ghent, 1:55-60.
 
[68]  Cohen, J. J. & Al-Rubeai, M. (1995): Apoptosis-targeted therapies the next big thing in biotechnology? Trends Biotechnol, 13: 281-283.
 
[69]  Dean, P. N. & Jett, J. H. (1974): Mathematical analysis of DNA distributions derived from flow microfluorometry. J. Cell. Biol, 60: 523-527.
 
[70]  Carleton, H. (1978): Histological Techniques, 4th Ed., London, Oxford, New York, Tornoto.
 
[71]  Manibusan, M. K., Odin, M. & Eastmond, D. A. (2007). Postulated carbon tetrachloride mode of action: a review. Journal of Environmental Science and Health Part C, 25(3): 185-209.‏
 
[72]  Mahran, M. Z., Ghada M. Elbassyouny. & Yousif A. Elhassaneen. (2018). Preventive effects of onion skin powder against hepatotoxicity in rats treated with benzo(a)pyrene. Proceeding of the Annual Conference (13th Arab; 10th International), 11-12 April, Faculty of Specific Education, Mansoura University, " Higher Education in Egypt and the Arab World in the Light of Sustainable Development Strategies", Mansoura, Egypt.
 
[73]  Susilo, R. J. K., Winarni, D., Husen, S. A., Hayaza, S., Punnapayak, H., Wahyuningsih, S. P. A. & Darmanto, W. (2019): Hepatoprotective effect of crude polysaccharides extracted from Ganoderma lucidum against carbon tetrachloride-induced liver injury in mice. Veterinary World, 12 (12): 1987- 1991.
 
[74]  Badawy, R. M. (2017). The effect of phytochemical extracts of some plant parts in liver cancer initiation induced by benzo(a)pyrene. Ph.D. Thesis in Nutrition and Farchood Science, Faculty of Home Economics, Minoufiya University, Egypt.
 
[75]  Elhassaneen, Y., Hassab El-Nabi, S. E., Bayomi, A. I. & ElKabary, A. R. (2022). Potential of watermelon (citrullis lanatus) peel extract in attenuating benzo[a]pyrene exposure-induced molecular damage in liver cells in vitro. Journal of Biotechnology Research, 8(3): 32-45.
 
[76]  Pagana, K. D. & Pagana, T. J. (1997). Mosby's diagnostic and laboratory test references. 3 rd ed., Mosby-year Book, Inc., New York.
 
[77]  Sayed Ahmed, S., Shehata, N. & Elhassaneen, Y. (2020). Potential Protective Effects of Ganoderma lucidum Powder against Carbon Tetrachloride Induced Liver Disorders in rats: Biological, Biochemical and Immunological Studies. Bulletin of the National Nutrition Institute of the Arab Republic of Egypt , 56(2): 99-132.
 
[78]  Elhassaneen, Y., Hassab El-Nabi, S., Mahran, M., Bayomi, A. and Badwy, E. (2022). "Potential Protective Effects of Strawberry (Fragaria Ananassa) Leaves Against Alloxan Induced Type 2 Diabetes in Rats: Molecular, Biological and Biochemical Studies". Sumerianz Journal of Biotechnology, 5(1): 1-15.
 
[79]  Elhassaneen, Y. A., Amal Z. Nasef., Rawan S. Arafa. & Asmaa I. Bayomi (2023): Bioactive compounds and antioxidant activities of milk thistle (Silybum marianum) extract and their potential roles in the prevention of diet-induced obesity complications. American Journal of Food Science and Technology, 11(3): 70-85.
 
[80]  Badawi, A. R. (2023). The effect of reishi mushroom (Ganoderma lucidum) on liver cancer induced by benzo[a]pyrene in rats. M.Sc. Thesis in Nutrition and Food Science, Faculty of Specific Education, Port Said University, Port Said, Egypt.
 
[81]  Christian, J. & Paul, G. (2011). Glycogen storage disease. Paediatrics and Child Health ,25(2):84-89.
 
[82]  Hasegawa, R., Chujo, T., Sai-Kato, K., Umemura, T., Tanimura, A. & Kurokawa, Y. (1995): Preventive effects of green tea against liver oxidative DNA damage and hepatotoxicity in rats treated with 2-nitropropane. Food Chem Toxicol, 33(11): 961-970.
 
[83]  Wang, F. R., Ai, H., Chen, X. M. & Lei, C. L. (2007): Hepatoprotective effect of a protein-enriched fraction from the maggots (Musca domestica) against CCl4-induced hepatic damage in rats. Biotechnol. Lett, 29(6):853-858.
 
[84]  Gutteridge, J. M. & Wilkins, S. (1983). Copper salt-dependent hydroxyl radical formation: damage to proteins acting as antioxidants. Biochimica et Biophysica Acta (BBA)-General Subjects, 759(1-2): 38-41.‏
 
[85]  Karim, E., Visser, M. E., John, J. P. & Stroes, E. S.(2009). Triglycerides and Cardiovascular Risk. Current Cardiology Reviews, 5(3):216-22.
 
[86]  Elbasouny, G., Shehata, N. and Elhassaneen, Y. (2019). Feeding of some selected food industries by-products induced changes in oxidants/antioxidant status, lipids profile, glucose and immunological parameters of blood obese rats. The 6th Scientific and 4th International Conference "The Future of Specific Education and people with Special Needs in Light of the Concept of Quality ", 24-26 February 2019, Faculty of Specific Education, Ain Sokhna University, El-Ain El-Soghna, Egypt.
 
[87]  Elhassaneen, Y., Ragab, S. & Essa, E. (2020).Chemical and nutritional studies on extracts of food processing by-products and their effects on obesity complications in rats. Journal of Home Economics, 30 (2): 1-26.
 
[88]  Yousif A. Elhassaneen., Reem A. Boraey. & Amal Z. Nasef (2023): Biological Activities of Ashwagandha (Withania somnifera L.) Roots and their Effect on the Neurological Complications of Obesity in Rats. American Journal of Food and Nutrition. 11(3): 71-88.
 
[89]  Asai, A. & Miyazawa, T. (2001). Dietary curcuminoids prevent high-fat diet–induced lipid accumulation in rat liver and epididymal adipose tissue. The Journal of nutrition, 131(11):2932-2935.‏
 
[90]  Chuengsamarn , S., Suthee, R., Benjaluck, P., Rungsunn, T. & Siwanon, J. (2014).Reduction of atherogenic risk in patients with type 2 diabetes by curcuminoid extract: a randomized controlled trial. J Nutr Biochem , 25(2):144-50.
 
[91]  Reed, D. J. & Beatty, P. W. (1980): in Reviews in Biochemical Toxicology, Vol. 2, E. Hodgson, J.R. Bend and R. Phillpot, Eds., Elsevier/North Holland, New York, 213 – 241.
 
[92]  Larsson, A., Orrenius, S., Holmgren, A. & Mannervik, B. (1983): Eds., Functions of glutathione, Raven Press, New York.
 
[93]  Halliwell, B. & Gutteridge, J.M. (1985): Free radicals in biology and medicine. Clarendon Press. Oxford. UK.
 
[94]  Elmaadawy, A., Rasha, M. Arafa. & Yousif, Elhassaneen. (2016). Oxidative Stress and antioxidant defense systems status in obese rats feeding some selected food processing by-products applied in bread. Journal of Home Economics, 26 (1): 55-91.
 
[95]  Elhassaneen, Y., Ghamry, H. & Lotfy, L. (2018). Potential Chemoprevention of Liver Disorders by Dietary Curcumin in Rats Treated with Benzo(a)pyrene. Proceeding of the 1st Scientific International Conference of the Faculty of Specific Education, Minia University, “Specific Education, innovation and labor market” 16-17 Juli, 2018, Minia, Egypt.
 
[96]  Galkina, O. V., Bakhtyukov, A. A., Akhmetshin, M. O., Prokopenko, V. M. & Eshchenko, N. D. (2017). The glutathione system in the subcellular fractions of developing rat brain and liver. Neurochemical Journal, 11(4): 266–271.
 
[97]  Shamberger, R. J., Andreone, T. L. and Willis, C. E. (1974): Antioxidants and cancer. IV. Malonaldehyde has initiating activity as a carcinogen. J. Natr. Cancer Inst. 53: 1771.
 
[98]  Michurina, S. A., Arkhipov, S. A. & Kolesnikov, S. I. (2014): Hepatocyte Apoptosis in Rats Exposed to Benzo(a)pyrene. Bulletin of Experimental Biology and Medicine. 158:150-152.
 
[99]  Xu, H., Yi, T., Liu, M., Gao, R., Liu, X., He, J., Ding, Y., Geng, Y., Mu, X., Wang, Y. & Chen, X. (2023): Exposure to Benzo(a)pyrene promotes proliferation and inhibits differentiation of stromal cells in mice during decidualization. Ecotoxicology and Environmental safety, 251.
 
[100]  Wellington, K. & Jarvis, B. (2001): Silymarin: a review of its clinical properties in the management of hepatic disorders. BioDrugs,15:465–89.
 
[101]  Lee, M., Huang, Z., Kim, D. J., Kim, S. H., Kim, M. O., Lee, S. Y., Xie, H., Park, S. J., Kim J. Y., Kundu, J. K., Bode, A. M., Surh, Y. J. & Dong, Z. (2013): Direct targeting of MEK1/2 and RSK2 by silybin induces cell cycle arrest and inhibits melanoma cell growth. Cancer Prev Res (Phila), 6: 455-465.
 
[102]  Kolade, O. Y. & Oladiji, T. A. (2019). Protective Effects Of Curcumin Against Benzopyrene Induced Liver Toxicity In Albino Rats. 8th International Biotechnology Conference, Exhibition and Workshop, IOP Conf. Series: Earth and Environmental Science 210, 012013, IOP Publishing, Gothenburg, Sweden.
 
Manuscript template