Antioxidant Activity of Extract from Sophora flavescens Solander ex Aiton in PC12 Cells

Hyun Woo Kang^1,

¹Department of Korean Food & Culinary Arts, Youngsan University, Busan, Korea

Cite this paper:
Hyun Woo Kang. Antioxidant Activity of Extract from Sophora flavescens Solander ex Aiton in PC12 Cells. Journal of Food and Nutrition Research. 2017; 5(4):205-207. doi: 10.12691/jfnr-5-4-1

Abstract

This study was investigated to antioxidant activity in human neuronal cell lines of extract from Sophora flavescens ex Aiton (ESSA). The cell viability showed the protective effect against oxidative damage to PC12 cell by MTT assay. We show that antioxidant activities of the ESSA were evaluated using apoptosis assays analyzed by flow cytometry. ESSA decreased cell death in PC12 cells due to hydrogen peroxide-induced oxidative damage in a dose-dependent manner. In addition, ESSA regulated the expression of Bcl-2 and Bax proteins in PC-12 cells. Based on these findings, we suggest that ESSA has the potential to inhibit hydrogen peroxide-induced free radical activation and should be considered in functional food and pharmaceutical applications.

Keywords:
Sophora flavescens ex Aiton antioxidant free radical neuronal cells

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References:

[1]	Kuroyanagi, M., Arakawa, T.Y., Hayashi, T., “Antibacterial and antiandrogen flavonoids from Sophora flavescens” J Nat Prod 62(12), 1595-1599. 1999.
[2]	Dong, W.K., Chi, Y.S., Son, K.H., Chang, H.W., Ju, S.K., Kang, S.S., Kim, H.P., “Effects of sophoraflavanone G, a prenylated flavonoid from Sophora flavescens, on cyclooxygenase-2 and in vivo inflammatory response” Arch Pharm Res 25(3), 329-335. 2002.
[3]	Matsuo, K., Ito, M., Honda, G., Qui, T.K., Kiuchi, F., “Trypanocidal flavonoids from sophora flavescens” Nat Med 57, 253-255. 2003.
[4]	Sies, H., “Oxidative stress: oxidants and antioxidants” Exp Physiol 82(2), 291-295. 1997
[5]	Darley-Usmar, V., Wiseman, H., and Halliwell, B., “Nitric oxide and oxygen radicals: A question of balance. FEBS Letters 309, 131-135. 1995.
[6]	Halliwell, B., “Free radicals, antioxidants, and human disease: Curiosity, cause, or consequence?” Lancet 344, 721-724. 1994.
[7]	Cook, N. C., and Samman, S., “Flavonoids - Chemistry, metabolism, cardioprotective effects, and dietary sources” Nutr Biochem 7, 66-76. 1996.
[8]	Lee, J., “Effect of soy protein and genistein on blood glucose, antioxidant enzyme activities, and lipid profile in streptozotocin-induced diabetic rats” Life Sci 79, 1578-1584. 2006.
[9]	Masia, M., Padilla, S., Bernal, E., Almenar, M.V., Molina, J., Hernandez, I., “Influence of antiretroviral therapy on oxidative stress and cardiovascular risk: A prospective cross-sectional study in HIV-infected patients” Clin Ther 29(7), 1448-1455. 2007.
[10]	Halliwell, B., “Role of free radicals in neurodegenerative diseases: Therapeutic implications for antioxidant treatment” Drugs Aging 18, 685-716. 2001.
[11]	Rattan, S.I.S., “Theories of biological aging: Genes, proteins, and free radicals” Free Radic Res 40(12), 1230-1238. 2006.
[12]	Lee, S.J., Kim, Y.S., Hwang, J.W., Kim, E.K., Moon, S.H., Jeon, B.T., Jeon, Y.J., Kim, J.M., and Park, P.J., “Purification and characterization of a novel antioxidative peptide from duck skin by-products that protects liver against oxidative damage” Food Res Int 49, 285-295. 2012.
[13]	Lee, S.J., Kim, E.K., Hwang, J.W., Oh, H.J., Cheong, S.H., Moon, S.H., Jeon, B.T., Park, P.J., “Purification and characterisation of an antioxidative peptide from enzymatic hydrolysates of duck processing by-products” Food Chem 123, 216-220. 2010.
[14]	Lee, S.J., Kim, K.H., Kim, Y.S., Kim, E.K., Hwang, J.W., Lim, B.O., Moon, S.H., Jeon, B.T., Jeon, Y.J., Ahn, C.B., Park, P.J., “Biological activity from the gelatin hydrolysates of duck skin by-products” Process Biochem 47, 1150-1154. 2012.
[15]	Lee, S.J., Lee, S.Y., Ha, H.J., Cha, S.H., Lee, S.K., and Hur, S.J., “Rutin Attenuates lipopolysaccharide-induced nitric oxide production in macrophage cells” J Food Nutr Res 3(3), 202-205. 2015.
[16]	Lee, S.J., Kim, E.K., Oh, H.J., Kwon, H.J., Hwang, J.W., Moon, S.H., Jeon, B.T., Park, P.J., Lim, B.O., “Free radical scavenging activity and protective effect against H2O2-induced stress in neuronal cells of enzymatic extracts from Sarcodon aspratus” Korean J Med Crop Sci 19(2), 77-82. 2011.
[17]	Lee, S.J., Kang, H.W., Lee, S.Y., and Hur, S.J., “Green tea polyphenol epigallocatechin-3-O-gallate attenuates lipopolysaccharide-induced nitric oxide production in RAW264.7 cells” J Food Nutr Res 2(7), 425-428. 2014.
[18]	Jin, J.H., Kim, J.S., Kang, S.S., Son, K.H., Chang, H.W., Kim, H.P., “Anti-inflammatory and anti-arthritic activity of total flavonoids of the roots of Sophora flavescens” J Ethnopharmacol 127, 589-595. 2010.
[19]	Bai, L., Zhu, L.Y., Yang, B.S., Shi, L.J., Liu, Y., Jiang, A.M., Zhao, L.L., Song, G., Liu, T.F., “Antitumor and immunomodulating activity of a polysaccharide from Sophora flavescens Ait” Int J Biol Macromol 51, 705-709. 2012.
[20]	Jung, J., Kang, S.S., Woo, J.J., Choi, J.S., “A new lavandulylated flavonoid with free radical and onoo-scavenging activities from Sophora flavescens” Arch Pharmacal Res 28, 1333-1336. 2005.
[21]	Kang, S.S., Kim, J.S., Son, K.H., Chang, H.W., Kim, H.P., “A new prenylated flavanone from the roots of Sophora flavescens” Fitoterapia 71, 511-515. 2000.