A 28-Day Diet Intervention in Obese Young Women with Different FTO rs9939609 Gene Did Not Impact Cardiovascular Inflammation Markers

Putri Novitasari^{1, 2}, Rimbawan Rimbawan^1, , Hardinsyah Hardinsyah¹ and Hadi Riyadi¹

¹Department of Community Nutrition, IPB University, Bogor, Indonesia

²Nutrition Study Program, Universitas Pendidikan Indonesia, Bandung, Indonesia

Cite this paper:
Putri Novitasari, Rimbawan Rimbawan, Hardinsyah Hardinsyah and Hadi Riyadi. A 28-Day Diet Intervention in Obese Young Women with Different FTO rs9939609 Gene Did Not Impact Cardiovascular Inflammation Markers. Journal of Food and Nutrition Research. 2025; 13(2):87-92. doi: 10.12691/jfnr-13-2-5

Abstract

“Variations in the FTO gene and specific dietary interventions have been linked to inflammatory and hormonal biomarker changes in obese individuals. This research focused on assessing the impact of a 28-day balanced diet intervention on inflammation markers associated with cardiovascular risk among different FTO rs9939609 gene single nucleotide polymorphisms (SNPs) in obese women. This randomized controlled trial involved 38 women aged 18–25 with high body fat, who were randomly categorized into four groups based on FTO rs9939609 SNP and balanced diet intervention for 28 days: Allele A carriers + diet (DAC, n=12), TT genotype + diet (DTH, n=8), Allele A carriers without diet (CAC, n=10), and TT genotype without diet (CTH, n=8). Participants underwent nutritional education and had interleukin-6 (IL-6), high-sensitivity C-reactive protein (hs-CRP), and adiponectin (ADP) measured pre- and post-intervention. The students' t-test statistical analyses were conducted using SPSS software. Initial IL-6 levels were highest in CAC (99.6 ± 29.4 ng/L) and lowest in DAC (76.4 ± 22.4 ng/L). Hs-CRP was highest in CTH (26.6 ± 9.48 ng/mL) and lowest in DTH (21.6 ± 6.0 ng/mL). ADP was highest in CTH (0.54 ± 0.08 ng/mL) and lowest in DAC (0.48 ± 0.10 ng/mL). After 28 days of balanced diet, none of the inflammatory markers showed statistically significant changes in all groups (P≥0.05). The balanced diet intervention did not significantly affect inflammatory markers linked to cardiovascular risk among all variants. Future studies should explore the prolonged effects of specific dietary components on inflammation and cardiovascular outcomes across diverse populations or other genes.”.

Keywords:
FTO rs9939609 inflammation cardiovascular risk obese women

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References:

[1]	Espeland, M., Zomer, E., Razquin, C., De Luis, D. A., Di Renzo, L., Luglio, H. F., & Parastouei, K., "Reduction in weight and cardiovascular disease risk factors in individuals with type 2 diabetes: One-year results of the Look AHEAD trial," Diabetes Care, 30 (6), 1374–1383, 2007.
[2]	Zomer, E., Razquin, C., Martinez, J. A., Martinez-Gonzalez, M. A., Bes-Rastrollo, M., Fernández-Crehuet, J., & Marti, A., "Interventions that cause weight loss and the impact on cardiovascular risk factors: A systematic review and meta-analysis," Obesity Reviews, 17 (10), 1001–1011, 2016.
[3]	Razquin, C., Martinez, J. A., Martinez-Gonzalez, M. A., Bes-Rastrollo, M., Fernández-Crehuet, J., & Marti, A., "A 3-year intervention with a Mediterranean diet modified the association between the rs9939609 gene variant in FTO and body weight changes," International Journal of Obesity, 34 (2), 266–272, 2010.
[4]	De Luis, D. A., Aller, R., Izaola, O., Primo, D., Urdiales, S., & Romero, E., "Evaluation of weight loss and adipocytokine levels after two hypocaloric diets with different macronutrient distribution in obese subjects with rs9939609 gene variant," Diabetes/Metabolism Research and Reviews, 28, 663–668, 2012.
[5]	De Luis, D. A., Aller, R., Izaola, O., Primo, D., Urdiales, S., & Romero, E., "Effects of a high-protein/low-carbohydrate diet versus a standard hypocaloric diet on weight and cardiovascular risk factors: Role of a genetic variation in the rs9939609 FTO gene variant," Journal of Nutrigenetics and Nutrigenomics, 8 (3), 128–136, 2015.
[6]	Luglio, H. F., Sulistyoningrum, D. C., Muharomin, I. R., & Huriyati, E., "Leptin, appetite and weight rebound in overweight/obesity individuals undertook weight loss program using a low-calorie diet with or without exercise," Medical Journal of Nutrition and Metabolism, 10 (3), 223–233, 2017.
[7]	Di Renzo, L., De Luis, D. A., Aller, R., Izaola, O., Primo, D., & Parastouei, K., "Influence of FTO rs9939609 and Mediterranean diet on body composition and weight loss: A randomized clinical trial NCT01890070," Journal of Translational Medicine, 16 (1), 1–12, 2018.
[8]	De Luis, D. A., Izaola, O., Primo, D., Lopez Gomez, J. J., & Aller, R., "RS9939609 FTO gene variant modified weight loss and insulin resistance after a partial meal-replacement hypocaloric diet," European Review for Medical and Pharmacological Sciences, 24 (10), 5573–5581, 2020.
[9]	Parastouei, K., De Luis, D. A., Izaola, O., Primo, D., Lopez Gomez, J. J., & Aller, R., "Gene-diet interaction of FTO-rs9939609 gene variant and hypocaloric diet on glycemic control in overweight and obese adults: A systematic review and meta-analysis of clinical trials," Chinese Medical Journal (English), 133 (3), 310–317, 2020..
[10]	Indonesian Ministry of Health, Regulation of the Minister of Health of the Republic of Indonesia Number 41 of 2014 on Pedoman Gizi Seimbang, Indonesian Ministry of Health, Jakarta, 2014.
[11]	Zimmermann, E., Magno, F. C. C. M., Hosseini, S., Fisher, E., Sun, Y., Lappalainen, T., & Mehrdad, M., "Influences of the common FTO rs9939609 variant on inflammatory markers throughout a broad range of body mass index," PLoS One, 6 (1), 1–6, 2011.
[12]	De Luis, D. A., Aller, R., Izaola, O., Primo, D., & Romero, E., "Association of the rs9939609 gene variant in FTO with insulin resistance, cardiovascular risk factor and serum adipokine levels in obese patients," Nutrición Hospitalaria, 33 (5), 1102–1107, 2016.
[13]	Magno, F. C. C. M., Zimmermann, E., Fisher, E., Sun, Y., Mehrdad, M., & Yang, F., "Influence of FTO rs9939609 polymorphism on appetite, ghrelin, leptin, IL6, TNFα levels, and food intake of women with morbid obesity," Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy, 11, 199–207, 2018.
[14]	Hosseini, S., Alipour, M., Zakerkish, M., Cheraghian, B., & Ghandil, P., "Effects of epigallocatechin gallate on total antioxidant capacity, biomarkers of systemic low-grade inflammation and metabolic risk factors in patients with type 2 diabetes mellitus: The role of FTO-rs9939609 polymorphism," Archives of Medical Science, 2020.
[15]	Fisher, E., Zimmermann, E., Sun, Y., Magno, F. C. C. M., Lappalainen, T., & Yang, F., "Association of the FTO rs9939609 single nucleotide polymorphism with C-reactive protein levels," Obesity, 17 (2), 330–334, 2009.
[16]	Sun, Y., Sun, J., Wang, X., & Yang, M., "Variants in the fat mass and obesity-associated (FTO) gene are associated with obesity and C-reactive protein levels in Chinese Han populations," Clinical and Investigative Medicine, 33 (6), 405–412, 2010.
[17]	Lappalainen, T., Fisher, E., Magno, F. C. C. M., Mehrdad, M., Yang, F., & Tupikowska-Marzec, M., "Association of the FTO gene variant (rs9939609) with cardiovascular disease in men with abnormal glucose metabolism - The Finnish Diabetes Prevention Study," Nutrition, Metabolism & Cardiovascular Diseases, 21 (9), 691–698, 2011.
[18]	Tupikowska-Marzec, M., Kolačkov, K., Zdrojowy-Wełna, A., Słoka, N. K., Szepietowski, J. C., & Maj, J., "The influence of FTO polymorphism rs9939609 on obesity, some clinical features, and disturbance of carbohydrate metabolism in patients with psoriasis," Biomedical Research International, vol. 2019, 2019.
[19]	Mehrdad, M., Doaei, S., Gholamalizadeh, M., Fardaei, M., Fararouei, M., & Eftekhari, M. H., "Association of FTO rs9939609 polymorphism with serum leptin, insulin, adiponectin, and lipid profile in overweight adults," Adipocyte, 9 (1), 51–56, 2020.
[20]	Yang, F., Lappalainen, T., Fisher, E., Magno, F. C. C. M., Tupikowska-Marzec, M., & Mehrdad, M., "Effect of epidemic intermittent fasting on cardiometabolic risk factors: A systematic review and meta-analysis of randomized controlled trials," Frontiers in Nutrition, 8 (October), 2021.
[21]	Saucedo, R., Isgin-Atici, K., Duicu, C., Lappalainen, T., De Luis, D. A., & Äijälä, M., "Gene variants in the FTO gene are associated with adiponectin and TNF-alpha levels in gestational diabetes mellitus," Diabetology & Metabolic Syndrome, 9 (1), 1–7, 2017..
[22]	Isgin-Atici, K., Duicu, C. O., MǍrginean, C. O., VoidǍzan, S., Tripon, F., & BǍnescu, C., "FTO gene–lifestyle interactions on serum adiponectin concentrations and central obesity in a Turkish population," International Journal of Food Sciences and Nutrition, 72 (3), 375–385, 2021.
[23]	Duicu, C., MǍrginean, C. O., VoidǍzan, S., Tripon, F., & BǍnescu, C., "FTO rs9939609 SNP is associated with adiponectin and leptin levels and the risk of obesity in a cohort of Romanian children population," Medicine (United States), 95 (20), 1–7, 2016.
[24]	Lappalainen, T., Duicu, C., De Luis, D. A., Saucedo, R., Äijälä, M., & Qi, L., "Gene expression of FTO in human subcutaneous adipose tissue, peripheral blood mononuclear cells, and adipocyte cell line," Journal of Nutrigenetics and Nutrigenomics, 3 (1), 37–45, 2010.
[25]	De Luis, D. A., Aller, R., Izaola, O., & Pacheco, D., "Relación del polimorfismo rs9939609 del gen FTO con factores de riesgo cardiovascular y niveles de adipocitoquinas en pacientes con obesidad mórbida," Nutrición Hospitalaria, 27 (4), 1184–1189, 2012.
[26]	De Luis, D. A., Aller, R., Izaola, O., & Pacheco, D., "Papel de la variante del gen FTO rs9939609 en la pérdida de peso, resistencia a la insulina y parámetros metabólicos tras dos dietas hipocalóricas, ricas en grasas monoinsaturadas vs polinsaturadas," Nutrición Hospitalaria, 32 (1), 175–181, 2015.
[27]	Äijälä, M., Lappalainen, T., De Luis, D. A., Duicu, C., Saucedo, R., & Qi, L., "The fat mass and obesity-associated (FTO) gene variant rs9939609 predicts long-term incidence of cardiovascular disease and related death independent of the traditional risk factors," Annals of Medicine, 47 (8), 655–663, 2015.
[28]	Carbone, S., Canada, J. M., Billingsley, H. E., Siddiqui, M. S., Elagizi, A., & Lavie, C. J., "Obesity paradox in cardiovascular disease: Where do we stand?" Vascular Health and Risk Management, 15, 89–100, 2019.
[29]	Qi, L., Saucedo, R., Lappalainen, T., De Luis, D. A., & Äijälä, M., "Fat mass-and obesity-associated (FTO) gene variant is associated with obesity; Longitudinal analyses in two cohort studies and functional test," Diabetes, 57 (11), 3145–3151, 2008.