Nrf2/HO-1-inductive Preventive Effect of 3,5-dicaffeoylquinic Acid, Antioxidant Compound from Green Coffee Beans, on Dimethylnitrosamine-induced Liver Fibrosis in Rats

Min A Kim^{1, 2}, Chul-Jun Lee^{1, 2}, Seon-ah Park^{1, 3}, Do Hun Kim^{1, 3}, Don-Haeng Lee² and Su-Geun Yang^{1, 3,}

¹Department of Biomedical Science and Program in Biomedical Science & Engineering, Inha University College of Medicine, Incheon 22212, Republic of Korea

²Division of Gastroenterology & Hepatology, Inha University Hospital, Incheon 22332, Republic of Korea

³Inha Institute of Aerospace Medicine, Inha University College of Medicine, Incheon 22212, Republic of Korea

Cite this paper:
Min A Kim, Chul-Jun Lee, Seon-ah Park, Do Hun Kim, Don-Haeng Lee and Su-Geun Yang. Nrf2/HO-1-inductive Preventive Effect of 3,5-dicaffeoylquinic Acid, Antioxidant Compound from Green Coffee Beans, on Dimethylnitrosamine-induced Liver Fibrosis in Rats. Journal of Food and Nutrition Research. 2020; 8(9):528-535. doi: 10.12691/jfnr-8-9-9

Abstract

In this study, we investigated the hepatoprotective properties of 3,5-dicaffeoylquinic acid (DQA), a polyphenolic compound from green coffee beans, against dimethylnitrosamine (DMN)-induced hepatic fibrosis rat models. DQA, up to 10 μM concentration, did not exhibit any cytotoxic effect on Chang liver cells and Huh7 cells. DMN-treated rats showed typical biological symptoms of hepatic fibrosis, i.e., loss of body weight and pathological elevation of serum biomarkers for hepatic function. However, oral administration of DQA recovered body weight, and maintained serum biochemical markers such as albumin, bilirubin, ALP, ALT and AST in the normal range. Notably, histological and western assay proved DQA significantly alleviated collagen accumulation (α-smooth muscle actin, alpha 1 collagen type I) in liver tissue. The upregulated tissue expression of erythroid 2–related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) suggested DQA upregulated the representative antioxidant enzymes and ameliorated the progression of hepatic fibrosis in DMN-treated rats.

Keywords:
35-dicaffeoylquinic acid Polyphenol Antioxidant Hepatic fibrosis Reactive oxygen species green coffee beans Nuclear factor erythroid 2-related factor 2 (Nrf2) pathway

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