The Alleviation Effect of Combination of Tempeh and Red Ginger Flour towards Insulin Sensitivity in High-Fat Diet Rats

Luthfia Dewi^1, , Lara Ayu Lestari², Azizah Nur Astiningrum¹, Vita Fadhilah¹, Nur Amala², Muh. Abdal B¹ and Nurul Hidayah¹

¹Department of Nutrition, Universitas Muhammadiyah Semarang, Semarang, Indonesia

²Department of Nutrition, Universitas Diponegoro, Semarang, Indonesia

Cite this paper:
Luthfia Dewi, Lara Ayu Lestari, Azizah Nur Astiningrum, Vita Fadhilah, Nur Amala, Muh. Abdal B and Nurul Hidayah. The Alleviation Effect of Combination of Tempeh and Red Ginger Flour towards Insulin Sensitivity in High-Fat Diet Rats. Journal of Food and Nutrition Research. 2020; 8(1):21-25. doi: 10.12691/jfnr-8-1-3

Abstract

High-fat diet is considered as a factor reducing insulin sensitivity. The purpose of this study was to evaluate the alleviation effect of the combination of tempeh and red ginger flour towards the insulin sensitivity represented by triglyceride-high density lipoprotein ratio in rats treated high-fat diet. Sprague Dawley male rats (n=30; body weight 150-200 g) were randomly divided by 5 groups (n=6), consisted K(-) group: group fed by normal diet (laboratory standard diet) as a control; K(+) group: group fed by high-fat diet; P1 group: group fed by high-fat diet and treated by tempeh flour/200 g body weight 1.9 g; P2 group: group fed by high-fat diet and treated by red ginger flour/200 g body weight 0.036 g; and P3 group: group fed by high-fat diet and treated by a combination tempeh flour/200 g body weight 0.95 g and red ginger 0.018 g. The treatments were given for 21 days. There was a significantly difference of the blood glucose and triglyceride-high density lipoprotein ratio (P<0.001), and P3 group was also showed the lowest level of glucose (96.21 mg/dL) and triglyceride-high density lipoprotein ratio (1.41) compared to K(+) (158.552±6.02 mg/dL; 6.02±0.36), P1 (103.71±1.79 mg/dL; 1.66±0.08), and P2 (108.46±2.61 mg/dL; 2.2±0.07). The combination of tempeh and red ginger flour promoted the improvement of blood glucose level and triglyceride-high density lipoprotein ratio better than the sole treatment.

Keywords:
glucose level insulin sensitivity red ginger tempeh

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References:

[1]	World Health Organisation, "Global report on diabetes", 2018.
[2]	M. Virally, J. F. Blicklé, J. Girard, S. Halimi, D. Simon, and P. J. Guillausseau, "Type 2 diabetes mellitus: epidemiology, pathophysiology, unmet needs and therapeutical perspectives", Diabetes Metab., vol. 33, no. 4, pp. 231–244, 2007.
[3]	A. D. von Frankenberg, A. Marina, X. Song, H. S. Callahan, M. Kratz, and K. M. Utzschneider, "A high-fat, high-saturated fat diet decreases insulin sensitivity without changing intra-abdominal fat in weight-stable overweight and obese adults", Eur. J. Nutr., vol. 56, no. 1, pp. 431-443, 2017.
[4]	K. Blaslov, F. S. Naranđa, I. Kruljac, and I. P. Renar, "Treatment approach to type 2 diabetes: Past, present and future", World J. Diabetes, vol. 9, no. 12, pp. 209-219, 2018.
[5]	W. C. Yeh, Y. C. Tsao, W. C. Li, I. S. Tzeng, L. S. Chen, and J. Y. Chen, "Elevated triglyceride-to-HDL cholesterol ratio is an indicator for insulin resistance in middle-aged and elderly Taiwanese population: A cross-sectional study", Lipids Health Dis., vol. 18, no. 1, pp. 1-7, 2019.
[6]	E. G. Behiry, N. M. El Nady, O. M. AbdEl Haie, M. K. Mattar, and A. Magdy, “Evaluation of TG-HDL Ratio Instead of HOMA Ratio as Insulin Resistance Marker in Overweight and Children with Obesity”, Endocrine, Metab. Immune Disord. - Drug Targets, vol. 19, no. 5, pp. 676-682, 2019.
[7]	B. Pantoja-Torres et al., “High triglycerides to HDL-cholesterol ratio is associated with insulin resistance in normal-weight healthy adults”, Diabetes Metab. Syndr. Clin. Res. Rev., vol. 13, no. 1, pp. 382-388, 2019.
[8]	V. Mani and L. C. Ming, “Tempeh and Other Fermented Soybean Products Rich in Isoflavones”, in Fermented Foods in Health and Disease Prevention, Elsevier Inc., 2017, pp. 453-474.
[9]	D. Y. Kwon, J. W. Daily, H. J. Kim, and S. Park, “Antidiabetic effects of fermented soybean products on type 2 diabetes”, Nutr. Res., vol. 30, no. 1, pp. 1-13, 2010.
[10]	M. Jayachandran and B. Xu, "An insight into the health benefits of fermented soy products", Food Chem., vol. 271, no. July, pp. 362-371, 2019.
[11]	S. Oh et al., "Ginger extract increases muscle mitochondrial biogenesis and serum HDL-cholesterol level in high-fat diet-fed rats", J. Funct. Foods, vol. 29, pp. 193-200, 2017.
[12]	A. M. Al Hroob, M. H. Abukhalil, R. D. Alghonmeen, and A. M. Mahmoud, "Ginger alleviates hyperglycemia-induced oxidative stress, inflammation and apoptosis and protects rats against diabetic nephropathy", Biomed. Pharmacother., vol. 106, no. June, pp. 381-389, 2018.
[13]	A. Vilela, “The importance of yeasts on fermentation quality and human health-promoting compounds”, Fermentation, vol. 5, no. 2, 2019.
[14]	Z. H. Cao, J. M. Green-Johnson, N. D. Buckley, and Q. Y. Lin, “Bioactivity of soy-based fermented foods: A review”, Biotechnol. Adv., vol. 37, no. 1, pp. 223-238, 2019.
[15]	X. Meng et al., "Dietary sources and bioactivities of melatonin", Nutrients, vol. 9, no. 4, pp. 1-64, 2017.
[16]	T. da S. M. de Farias et al., "Melatonin Supplementation Decreases Hypertrophic Obesity and Inflammation Induced by High-Fat Diet in Mice", Front. Endocrinol. (Lausanne)., vol. 10, no. November, pp. 1-13, 2019.
[17]	S. H. Bintari, N. D. Putriningtyas, K. Nugraheni, N. S. Widyastiti, E. Dharmana, and A. Johan, “Comparative effect of Tempe and soymilk on fasting blood glucose, insulin level and pancreatic beta cell expression (Study on streptozotocin-lnduced diabetic rats)”, Pakistan Journal of Nutrition, vol. 14, no. 4. pp. 239-246, 2015.
[18]	M. H. El Gayar, M. M. M. Aboromia, N. A. Ibrahim, and M. H. Abdel Hafiz, "Effects of ginger powder supplementation on glycemic status and lipid profile in newly diagnosed obese patients with type 2 diabetes mellitus", Obes. Med., vol. 14, no. April, 2019.
[19]	J. Zhu, H. Chen, Z. Song, X. Wang, and Z. Sun, "Effects of Ginger (Zingiber officinale Roscoe) on Type 2 Diabetes Mellitus and Components of the Metabolic Syndrome: A Systematic Review and Meta-Analysis of Randomized Controlled Trials", Evidence-based Complement. Altern. Med., vol. 2018, 2018.
[20]	C. K. Wei et al., "6-Paradol and 6-Shogaol, the Pungent Compounds of Ginger, Promote Glucose Utilization in Adipocytes and Myotubes, and 6-Paradol Reduces Blood Glucose in High-Fat Diet-Fed Mice", Int. J. Mol. Sci., vol. 18, no. 1, pp. 1-18, 2017.
[21]	A. Saedisomeolia et al., “Mechanisms of action of ginger in nuclear factor-kappaB signaling pathways in diabetes”, J. Herb. Med., vol. 16, 2019.
[22]	Y. Li, V. H. Tran, C. C. Duke, and B. D. Roufogalis, “Gingerols of zingiber officinale enhance glucose uptake by increasing cell surface GLUT4 in cultured L6 myotubes”, Planta Med., vol. 78, no. 14, pp. 1549-1555, 2012.
[23]	Y. Isa et al., “6-Shogaol and 6-gingerol, the pungent of ginger, inhibit TNF-α mediated downregulation of adiponectin expression via different mechanisms in 3T3-L1 adipocytes”, Biochem. Biophys. Res. Commun., vol. 373, no. 3, pp. 429-434, 2008.