Free Radical Scavenging Activities and Neuro-protective Effects of Extracts from Juniperus rigida Sieb. Fruit

Hyun Woo Kang^1, and Sang Jae Kwak¹

¹Department of Korean Food & Culinary Arts, Youngsan University, Busan, 48015, Korea

Cite this paper:
Hyun Woo Kang and Sang Jae Kwak. Free Radical Scavenging Activities and Neuro-protective Effects of Extracts from Juniperus rigida Sieb. Fruit. Journal of Food and Nutrition Research. 2019; 7(3):187-190. doi: 10.12691/jfnr-7-3-2

Abstract

The antioxidative activity of extracts from Juniperus rigida Sieb. Fruit (JRSF) was evaluated by measuring 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl and alkyl radical scavenging activity using an electron spin resonance (ESR) spectrometer. The free radical scavenging activity of the extract was dose dependant manner, and the IC₅₀ value on DPPH, hydroxyl and alkyl radical at 16.84, 82.35 and 52.27 μg/mL respectively. In addition, evaluated the protection effect on H₂O₂-induced oxidative damage in PC-12 cells via propidium iodide (PI) staining using a flow cytometer. JRSF extract decreased cell death in PC-12 cells due to H₂O₂-induced oxidative damage in a dose-dependent manner. These results suggest that JRSF extract exhibit antioxidative activity against oxidative stress on PC-12 cells.

Keywords:
Juniperus rigida Sieb. fruit antioxidant free radical PC-12 cells flow cytometer

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