Effect of Aqueous Extract of Euphorbia hirta (Euphorbiaceae) Leaves on Wistar Lactating Rat's Milk Density, Water and Fat Contents

Bruno Koffi KOKO^1, , Prisca Joelle DJOMAN², Jeanne Akoua KANGA² and Brou André KONAN¹

¹Biology and Health Laboratory, Félix Houphouët-Boigny University, Abidjan, Côte d’Ivoire

²Alassane Ouattara University, Bouaké, Côte d’Ivoire

Cite this paper:
Bruno Koffi KOKO, Prisca Joelle DJOMAN, Jeanne Akoua KANGA and Brou André KONAN. Effect of Aqueous Extract of Euphorbia hirta (Euphorbiaceae) Leaves on Wistar Lactating Rat's Milk Density, Water and Fat Contents. American Journal of Pharmacological Sciences. 2025; 13(2):38-42. doi: 10.12691/ajps-13-2-2

Abstract

Background and Objectives: Breast milk is highly recommended due to its nutritional composition, which adapts to the needs of the child. Unfortunately, many mothers do not have access to synthetic galactagogues due to the high cost of medications. To avoid a decrease or interruption of breast milk production, they mostly resort to medicinal plants such as Euphorbia hirta (Euphorbiaceae). The objective of this work is to evaluate the effect of oral administration of the aqueous extract of the leaves of E. hirta (EHae) on the water and fat composition of breast milk. Methods and Study Design: 36 lactating rats divided into seven (7) experimental groups are treated regularly from the third to the seventeenth day of postpartum. Group I received only distilled water. Group II was treated only with Metoclopramide, Prokynil®. The experimental groups (III; IV; V et VI) were treated with EHea at doses of 100; 200; 400 and 600 mg/kg respectively. Each rat received orally 1 ml of the substance administered using a gavage tube. Results: At doses of 100; 200; 400 and 600 mg/kg, EHae induced a very significant increase in the water content of milk without, however, significantly modifying its composition in terms of fat and other compounds. Conclusions: The use of EHae at doses lower than 400 mg/kg could ensure that breastfeeding mothers have better breast milk production.

Keywords:
Euphorbia hirta leaves breastfeeding milk composition lactating rats

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References:

[1]	UNICEF (United Nations Children's Fund), A Good Departure in the Life (In French), United Nations Plaza New York, 2010, 20 p. https://www.unicef.fr/sites/default/files/userfiles/Unicef_Rapport_annuel_international_2010. pdf.
[2]	Mosca, F. and Giannì, M.L, “Human Milk: Composition and Health Benefits,” La Pediatria Medicale Chirurgica, 39 (155). 47-52. Jun. 2017.
[3]	Hamosh, M, “Bioactive Factors in Human Milk,” Pediatric Clinics of North America, 48 (1). 69-86. Feb. 2001.
[4]	Cai, X., Wardlaw, T. and Brown, D.W, “Global Trends in Exclusive Breastfeeding,” International Breastfeed Journal, 7 (12). 5p. Sep.2012.
[5]	Forinash, A., Yancey, A., Barnes, K.N. and Myles T.D,“The Use of Galactogogues in the Breastfeeding Mother, ”Annals of Pharmacotherapy, 46 (10). 1392-1404. Sep.2012.
[6]	Koko, B.K., Konan, A.B., Djetouan, J.M.K., Kouacou, F.K.A., Kanga J. A., Kouakou K. L. and Amonkan, A.K. (2019) “Ethnobotanic Survey of the Galactogogue Plants Used by Brong and koulango, Two Indigenous People in Gontougo Region/Côte d’Ivoire,” International Journal of Phytomedecine, 11 (1). 015-022. Aug. 2019.
[7]	Koko, B.K., Konan, A.B., Kouacou, F.K.A., Djetouan, J.M.K. and Amonkan, A.K, “Galactagogue Effect of Euphorbia hirta (Euphorbiaceae) Aqueous Leaf Extract on Milk Production in Female Wistar Rats,” Journal of Biosciences and Medicines, 7. 51-65. Sep. 2019.
[8]	Nene-Bi, S.A., Traoré, F., Zahoui, O.S. and Soro, T.Y, “Phytochemical Composition, Pharmacological and Toxicological Studies of an Aqueous Extract of Bridelia ferruginea benth. (Euphorbiaceae) in Mammalians,” Afrique Science, 4 (2). 287-305. Nov. 2010.
[9]	Konan, A.B., Amonkan, A.K., Ahui, M.L.B., Bouafou, K.G.M., Kouakou, L.K., Kpahé, F.Z. and Datté, J.Y, “Myostimulating Effects of Sesamum radiatum Aqueous Leaf Extract in Isolated Guinea Pig Taenia caeci Contractile Activity,” African Journal of Traditional, Complementary and Alternative Medicine, 8 (4). 377-385. Dec. 2007.
[10]	Konan, A.B., Bouafou, K.G.M., Kouakou, L.K., Bléyéré, M.N., Amonkan, A.K., Zannou-Tchoko, J.V., Ahui, M.L.B. and Datté, J.Y, “Effects of Sesamum ra diatum Aqueous Leaf Extract on Rhythmic Contractions of Uterine Smooth Muscle Bundles from Pregnant Rats,” International Journal of Life Science and Medical Research, 2 (4). 82-89. Dec. 2012.
[11]	Ahui, M.L.B., Konan, A.B., Zannou-Tchoko, J.V., Amonkan, A.K., Kati-Coulibaly, S. and Offoumou, M.A. “Identification of Gingerols in Ginger (Zingiber officinale Roscoe) by High Performance Liquid Chromatography-Tandem Mass Spec trometry and Pharmacologic Studies of Its Aqueous Extract on the Rabbit Isolated Duodenum Contractility,” Journal of Physiology and Pharmacology Advances, 3. 16-26. Jan. 2013.
[12]	Konan, A.B., Ahui, M.L.B., Saraka, A.I., Amonkan, A.K., Kouakou, K.L., Bléyéré, M.N. and Datté, J.Y. “In Vitro Effects of Hydroethanolic Extract from Roots Bark of Parquetina nigrescens (Periplocaceae) on Rabbit Duodenum Contractile Activity,” World Journal of Pharmaceutical Research, 3 (1). 139-150. Apr. 2014. .
[13]	Garg, R. and Gupta, V.B., “A Comparative Study on Galactogogue Property of Milk and Aqueous Decoction of Asparagus racemosus in Rats,” International Journal of Pharmacognosy and Phytochemical Research, 2 (2). 36-39. Aug. 2010. http:// impactfactor.org/ PDF/ IJPPR/ 2/ IJPPR,Vol2, Issue2, Article2. pdf.
[14]	14. Keen, C.L., Lã-Nnerdal B., Clegg M. and Hurley L.S, “Developmental Changes in Composition of Rat Milk: Trace Elements, Minerals, Protein, Carbohydrate and Fat,” The Journal of. Nutrition, 111 (2). 226-230. Feb.1981.
[15]	Yagil, R., Lerner, Z., Etzion, Z. and Berlyne, G.M, “Acid-base Changes in Milk and Blood of Rats in Acidosis and Alkalosis,”. American Journal of Physiology, 231 (1). 132-135. Jul.1976.
[16]	Parmar, P., Lopez-Villalobos, N., Tobin, J.T., Murphy, E., McDonagh, A., Crowley, S.V., Kelly A.L. and Shalloo, L, “The Effect of Compositional Changes Due to Seasonal Variation on Milk Density and the Determination of Season-Based Density Conversion Factors for Use in the Dairy Industry”. Foods, 9 (8). 1004. Jul. 2020.
[17]	Liu, H.,Ying, Y., Luo, H., Shen, Z., Tao X. and Zhu, X, “An Herbal Galactagogue Mixture Increases Milk Production and Aquaporin Protein Expression in the Mammary Glands of Lactating Rats,” Evidence-Based Complementary and Alternative Medicine, Article ID 760585 6 p. May.2015.
[18]	Li, M., Ma R.N., Li, L.H., Qu, Y.Z. and Gao, G.D. “Astragaloside IV Reduces Cerebral Edema Post-ischemia/Reperfusion Correlating the Suppression of MMP-9 and AQP4,” European Journal of Pharmacology, 715 (1-3). 189-195. Sep. 2013.