Effect of the Fractions of Hydro-ethanolic Extract of Strychnos Camptoneura (Loganiaceae) Leaves on Acute and Chronic Inflammation in Wistar Rats

Silaho-Maleka Emmanuellia-Divine^{1, 2}, Elion Itou Romaric De Garde², Gombe Assoungou Herman^{1, 2} and Morabandza Cyr Jonas^{1, 2,}

¹Laboratory of Microbiology, Infectiology and Immunology, Higher Normal School, Marien Ngouabi University B.P69, Brazzaville (Congo)

²Laboratory of Pharmacodynamics and Experimental Physiopathology, Faculty of Science and Technology, Marien Ngouabi University B.P69, Brazzaville (Congo)

Cite this paper:
Silaho-Maleka Emmanuellia-Divine, Elion Itou Romaric De Garde, Gombe Assoungou Herman and Morabandza Cyr Jonas. Effect of the Fractions of Hydro-ethanolic Extract of Strychnos Camptoneura (Loganiaceae) Leaves on Acute and Chronic Inflammation in Wistar Rats. American Journal of Pharmacological Sciences. 2024; 12(4):51-55. doi: 10.12691/ajps-12-4-2

Abstract

S. camptoneura is a plant of the traditional Congolese pharmacopoeia known for its anti-inflammatory properties. This work is a comparative study of the effect of the hydro-ethanolic extract of S. camptoneura (Loganiaceae) leaves and its different fractions in order to identify the most effective fraction against acute and chronic inflammation. The fractions (F1, F2, F3 and F4) were obtained after fractionation of the hydro-ethanolic extract by open column chromatography. The acute and chronic anti-inflammatory effect was evaluated by injection of 2.5% formaldehyde, at the level of the plantar fascia in the Wistar rat 1 hour after administration of the extract and fractions at doses of 100 and 200mg /Kg, Diclofenac (10mg/Kg) and distilled water (1mL/100g). Edema volume (mm) was measured using an electronic caliper at 1/2, 1, 2, 3, 4 and 24 h after induction of inflammation. The obtained results showed that at doses of 100 and 200 mg/kg the extract, fractions and Diclofenac significantly inhibit (p<0.001) acute inflammation at 82.80 and 83.45% for F4; 81.64% for Diclofenac. Similarly, a significant inhibition (p<0.001) was observed with F4, F3 at 200 mg/Kg and Diclofenac at 83.02; 71.82 and 77.94% respectively. At J9, the inhibition was non-significant with the extract, F1 and F2 against a significant inhibition (p<0.05) with Diclofenac, F3 and F4 compared to distilled water (negative control). The inhibitions went from 77.94 to 35.60%; 71.82 to 65.45% and from 83.02 to 62.16% from J3 to J9 respectively for Diclofenac, F3 and F4. The maximum anti-inflammatory effect was obtained with the F4 fraction. These results show that the use of fractions in the care of inflammation would be a good alternative.

Keywords:
S. camptoneura Diclofenac fraction inflammation formaldehyde

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References:

[1]	Cheriti, A., Rahmani, S. & Belboukhari, N.. Evaluation of the anti-inflammatory activity of aqueous extracts of Limoniastrum feei (Plumbaginacea) leaves. Algerian Journal of Arid Environment “AJAE”, 6(1), 80-86, 2016.
[2]	Viladomiu, M., Hontecillas, R. & Bassaganya-Riera J. Modulation of inflammation and immunity by dietary conjugated linoleic acid. European journal of pharmacology,785, 87-95, 2016.
[3]	Kouadio Kouakou J., Ouattara-Soro F. S., Abizi G., Zougrou N’guessan E., Kouakou K. R., Begbin Kouassi E, Kplé T. K. M., Kablan Kassi J-J. and Koffi S. Anti-Inflammatory Activity and Phytochemical Studies of the Aqueous Extract of the Barks of Distemonanthus Benthamianus Baill. (Caesalpiniaceae: Leguminosae - Caesalpinioideae). European Scientific Journal, ESJ, 17(7), 74, 2021.
[4]	Soubrier, M., Rosenbaum, D. & Tatar, Z. Nonsteroidal Anti-Inflammatory Drugs and Vessels. Rheumatism Review, 80(3), 204-208, 2013.
[5]	Seck M., Sall C., Gueye P.M., Seck I., Dioum M.D., Lembachar Z., Gueye R.S., Fall D., and Dieye T.N., 2015. Study of the antisickling activity of root extracts of Leptadenia hastata Decne (Asclepradaceae). Int.J.Biol.Chem.Scie. 9(3): 1375-1383, 2015.
[6]	Tangara D, Diop A, Tirera H, Yaranga B, Diop M. Senegalese medicinal plant: determination of nutritional compositions and characterization of phytochemicals of Borreria verticillata. 2022; 172: 10.
[7]	Ribeiro SM, Fratucelli ÉDO, Fernandes JM, Bueno PCP, Cavalheiro AJ, Klein MI. Systematic approach to identify new antimicrobial and antibiofilm molecules from plant extracts and fractions to prevent dental caries. J. Vis. Exp. (169), e61773.
[8]	Akroum S.., «Analytical and Biological Study of Natural Flavonoids» Univ. Constantine, (2011).
[9]	Quy Diem Do, Elisa Angkawijaya, Phuong Lan Tran Nguyen, Lien Huong Huynh, Felycia Edi Soetaredjo, Suryadi Ismadji, Yi-Hsu Ju. Effect of extraction solvent on the total phenol content, total flavonoid content and antioxidant activity of Limnophila aromatica. Journal of Food and Drug Analysis. Vol : 22, No3, 296-302, (2014).
[10]	Koffi E., Sea T., Dodehe Y. and Soro S. Effect of solvent type on extraction of polyphenols from twenty three Ivorian plants. J.Animal & Plant Sci. Flight. 5, pp. 550-558. (2010).
[11]	Katalinic V., Mozina S., Skroza D., Generalic I., Abramovic H., Milos M., Ljubenkov I., Piskernik S., Pezo I., Terpinc P. and Boban M. Polyphenolic profile, antioxidant properties and antimicrobial activity of grape skin extracts of 14 Vitis vinifera varieties grown in Dalmatia (Croatia). J.Food. Chem. Flight. 119pp. 715-723. (2010).
[12]	Okamba Ondzia F.R, Morabandza C.J., Moulari B., Gombe Assoungou H., Abena A.A. Immunomodulatory effect of the aqueous extract of Strychnos camptoneura (Loganiaceae) leaves in Swiss mice11(1), p.1-8, (2022).
[13]	Morabandza C.J., Amboyi G.S., Matini L., Gouolali T., Elion Itou R.D.G., Etou Ossibi W.A., Andzi Barhé T., Ongoka P.R. and Abena A.A, ¨ Phytochemical constituents and antioxidant activity of Strychnos camptoneura Gilg and Busse (Loganiaceae) leaves. ¨ International Journal of current research, 9(03) pp47790-47794. (2017).
[14]	Elion Itou R.D.G, Etou Ossibi A.W, Epa C., Nsondé Ntandou G.F, Bokia C.B, Ouamba J.M, Abena A.A, 2017. Anti-inflammatory and analgesic effects of Chromolaena odorata L. leaves (King and Robinson). Vol .11(7). pp217-223, (2017).
[15]	Mendoza S., Noa M., Valle M., Mendoza N., Mas R. Effect of D-002 on formaldehyde-induced arthritis in rats ; IOSR journal of pharmacy, Vol.13, Issue 7 p9-12, (2013).
[16]	Ndiaye Sy A, Fall A.D, Ndiaye M, Sall A.O, Sy G.Y, Bassène E. and Dièye A.M. Demonstration of the anti-inflammatory activity of methanolic subfractions of Moringa oleifera Lam. (Moringaceae) leaves in rats. Int. J. Biol. Chem. Sci. 10(2): 760-768, (2016).
[17]	Moulari B., Morabandza C.J., Gombe Assoungou H., Abena A.A, 2023 ¨In vitro evaluation of the healing activity of the ethanolic extract of Strychnos camptoneura grains (Loganiaceae)¨. Volume 37, February 2023, 100624, (2023).
[18]	Emeraux E. Biological properties of flavonoids: bibliographic study and evaluation of antioxidant activity. hal-03297878:85, (2019).