Cytoprotective Activity of a Herbal Formulation on Induced Gastric Ulcers in Wistar Rats

Oussou N’Guessan Jean-Baptiste^1, , Kouakou Kouakou Léandre¹, Ehile Ehilé Hervé¹, Yoboue Kouamé Landry¹, Yapo Angoué Paul¹ and Ehile Ehouan Etienne¹

¹Laboratory of Physiology, Pharmacology and Pharmacopoeia, NANGUI ABROGOUA University, PO Box 801 Abidjan, Côte d’Ivoire

Cite this paper:
Oussou N’Guessan Jean-Baptiste, Kouakou Kouakou Léandre, Ehile Ehilé Hervé, Yoboue Kouamé Landry, Yapo Angoué Paul and Ehile Ehouan Etienne. Cytoprotective Activity of a Herbal Formulation on Induced Gastric Ulcers in Wistar Rats. American Journal of Pharmacological Sciences. 2023; 11(1):15-20. doi: 10.12691/ajps-11-1-3

Abstract

Macaranga barteri and Terminalia superba are two plants from the Ivorian flora used in traditional medicine to treat gastric disorders. This study aims to evaluate the cyprotective effect of a herbal formulation based on these herbs (AEMbTs) against induced ulcers in rats. The cytoprotective effect of AEMbTs was carried out on HCl/Ethanol and ibuprofen rats’ models. For each model, nine groups of six rats each were formed. The rats were orally pretreated with distilled water (Groups I and II), those of groups III and IV received Cimetidine (12 mg/kg bw) and Maalox (50 mg/kg bw) respectively in HCl/Ethanol model while Misoprostol (0,012 mg/kg bw) and Ranitidine (50 mg/kg bw) were respectively used as standard drugs in ibuprofen model. The rats in groups V to IX received AEMbTs at the respective doses of 31.25; 62.50; 125; 250 and 500 mg/kg bw. In the HCl/ethanol model, one hour after administration of the various treatments, all groups except group I received HCl/Ethanol orally at 1 ml/150 g bw. All the animals were sacrificed one hour later. As for the ibuprofen model, thirty (30) minutes after the treatments, all the animals except those of group 1 received orally a first dose of ibuprofen (500 mg/kg bw). Fifteen hours after the first dose, a second dose of ibuprofen was administered. Six (6) hours later, all the animals were sacrificed, the mucus was collected and weighed, the ulcerations were measured, the ulceration index and the inhibition percentage of the ulcerations were calculated. The results showed that the doses of AEMbTs induced significant decrease (p<0.05) of the ulceration areas. The inhibition values were 99.29 and 100%, respectively for the ibuprofen and HCl/ethanol models at the extract dose of 500 mg/kg bw. AEMbTs, also, significantly (p<0.001) increased mucus production. These results suggest that the herbal formulation has cytoprotective effects on the rat stomach and could justify the use of these plants by traditional healers to treat gastric disorders.

Keywords:
Macaranga barteri Terminalia superba HCl/Ethanol ibuprofen gastric ulcer

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References:

[1]	Wallace, J.L., McKnight, W., Reuter, B.K., Vergnolle, N. NSAID-induced gastric damage in rats: Requirement for inhibition of both cyclooxygenase 1 and 2. Gastroenterology, 119(3): 706-714. 2000.
[2]	Chauhan, A.K., Kang, S.C. Therapeutic potential and mechanism of thymol action against ethanol-induced gastric mucosal injury in rat model. Alcohol, 49: 739-745. 2015.
[3]	Yeomans, N.D., Hawkey, C.J.; Brailsford, W., Naesdal, J. Gastroduodenal toxicity of low-dose acetylsalicylic acid: A comparison with non-steroidal anti-inflammatory drugs. Curr. Med. Res. Opin., 25, 2785-2793. 2009.
[4]	Zhou, D., Yang, Q., Tian, T., Chang, Y., Li, Y., Duan, L. R. Gastroprotective effect of gallic acid against ethanol-induced gastric ulcer in rats: involvement of the Nrf2/HO-1 signaling and anti-apoptosis role. Biomed. Pharmacother., 126, 110075. 2020.
[5]	Lanas, A, Chan, F.K.L. Peptic ulcer disease. Lancet, 390 (10094): 613-624. 2017.
[6]	Doffou, A.S., Kouamé, G.D., Bangoura, A.D., Yaogo, A.M., Mamonman, K.H., Alassan, M.K., Yao-Bathaix, F.M., Attia, K.A. Prévalence des ulcères gastroduodénaux et des lésions précancéreuses gastriques au cours de la gastrite chronique à Helicobacter Pylori selon le système de sydney: À Propos de 52 Cas. Health Sciences and Diseases; 21 (3): 16-25. 2020.
[7]	OMS. Promotion du rôle de la médecine traditionnelle dans le système de santé: stratégie de la région africaine. AFR/RC50/9, 12-15. 2002.
[8]	Adesegun, S., Elechi, N. and Coker, H. Antioxidant power of Macaranga barteri leaf. American journal of food technology, 2(6):543-549. 2007.
[9]	Dougnon, T.V., Klotoe, J.R., Anago, E., Yaya, N. S., Fanou, B., Loko F. Antibacterial and Wound Healing Properties of Terminalia superba Engl. And Diels (Combretaceae) in Albino Wistar. Journal of Bacteriology and Parasitology, 5:5. 2014.
[10]	Ehilé, E.H., Goze, N.B., Kouakou, K.L., Yapo, A.P., Ehilé, E.E. Acute toxicity and gastric anti-ulcer activity of an aqueous extract of the leaves of Macaranga barteri Müll.Arg (Euphorbiaceae) on rat models. Journal of medicinal plants research, 12: 96-105. 2018.
[11]	Goze, N.B., Kouakou, K.L., Bléyéré, N.M., Amonkan, K.A., Konan, B.A., Abo, K.J.C., Ehilé, E.E. Anti-ulcerogenic effects of a hydroethanol 70% extract from stem bark of Terminalia superba Engl. Et Diels (Combretaceae) in rat and phytochemical screening. Iinternational journal of science innovation and discoveries, 3(5): 539-550. 2013.
[12]	Anonymous. National Research Council. Guide for the Care and Use of Laboratory Animals. Washington: National Academy Press. 1996.
[13]	Kouakou, K.L., Goze, N.B., Bleyere, N.M., Konan, B.A., Amonkan, K.A, Abo, K.J-C., Yapo, A.P., Ehile, E.E. Acute toxicity and anti-ulcerogenic activity of an aqueous extract from the stem bark of Terminalia superba Engl. and Diels (Combretaceae). World Journal of Pharmaceutical Sciences; 1(4): 117-129. 2013.
[14]	Hara, N., Okabe, S. Effects of Gefanate on acute lesions in rats. Folia Pharmacologica Japonica, 85: 443-448. 1985.
[15]	Robert, A., Nezamis, J.E., Lancaster, C., Davis, J.P., Field, S.O., Hanchar, A.J. Mild irritants prevent gastric necrosis through ‘adaptive cytoprotection’ mediated by prostaglandins. American Journal of Physiology-Gastrointestinal and Liver Physiology, 245: 113-121. 1983.
[16]	Nguelefack, T.B., Watcho, P., Wansi, S.L., Nguelta, M.M., Ngamga, D., Tane, P., Kamanyi A. The antiulcer effect of the methanol extract of the leaves of Aspilia Africana (Asteraceae) in rats. African Journal of traditional, Complementary and Alternative Medicines, 2: 233-237. 2005.
[17]	Parmar, N.S., Desai, J.K. A review of current methodology for the evaluation of gastric and duodenal antiulcer agents. Indian Journal of Pharmacoly, 25:120-135 1993.
[18]	Kulkarni, S.K. Hand Book of Experimental Pharmacology. Vallabh Prakashan, New Delhi, India, 3rd edition, pp 236-238. 2002.
[19]	Marhuenda, E., Martin, M.J., Lastra, C.D.L.A. Antiulcerogenic activity of aescine in different experimental models. Phytotherapy Research., 7: 13-16, 1993.
[20]	Oates, P.J, Hakkinen, J.P. Studies on the mechanism of ethanol induced gastric damage in rats. Gastroenterology, 94:10-21 1988.
[21]	Jelski, W., Kozlowski, M., Laudanski, J., Niklinski, J., Szmitkowski, M. The activity of class I, II, III, and IV alcohol dehydrogenase (ADH) isoenzymes and aldehyde dehydrogenase in pancreatic cancer. Pancreas; 35(2): 142-146, 2009.
[22]	Gadekar, R., Singour, P.K., Chaurasiya, P.K., Pawar, R.S., Patil, U.K. A potential of some medicinal plants as antiulcer agents. Pharmacognosy reviews, 4(8): 136-146. 2010.
[23]	Sharifi-Rad, M., Fokou, P., Sharopov, F., Martorell, M., Ademiluyi, A.O., Rajkovic, J., Salehi, B., Martins, N., Iriti, M., Sharifi-Rad, J. Antiulcer agents: from plant extracts to phytochemicals in healing promotion. Molecules, 23(7): 1751-1759, 2018.
[24]	Borrelli, F., Izzo, A.A. The plant kingdom as source of antiulcer remedies. Phytotherapy Research; 14: 581-591 2000.
[25]	Pious, S.T., Koilpitchai, P., Veerabahu, R.M. Evaluation of antiulcer activity of ethanol extracts of Aristolochia krisagathra Sivarajan and Pradeep and Aristolochia bracteata Retz. whole plants in experimental rats. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 7(4): 1-6. 2016.
[26]	Beck, W.S., Schneider, H.T., Dietzel, K., Nuernberg B., Brune K. Gastrointestinal ulcerations induced by anti-inflammatory drugs in rats. Physicochemical and biochemical factors involved. Archive of Toxicology; 64(3): 210-217. 1990.
[27]	Wallace, J.L. Prostaglandins, NSAIDs, and gastric mucosal protection: why doesn't the stomach digest itself? Physiol Rev., 88:1547-1565. 2008.
[28]	Sandvik, A.K., Brenna, E., Waldum, H.L. Review article: the pharmacological inhibition of gastric acid secretion tolerance and rebound. Alimentary Pharmacology &Therapeutics, 11: 1013-1018. 1997.
[29]	Scheiman, J.M. The use of proton pump inhibitors in treating and preventing NSAID-induced mucosal damage. Arthritis Research & Therapy; 15(3): S5. 2013.
[30]	Gwee, K.A., Goh, V., Lima, G., Setia, S. Coprescribing proton-pump inhibitors with nonsteroidal anti-inflammatory drugs: risks versus benefits. Journal of Pain Research, 11:361-374. 2018.
[31]	Tejasvi, R.V., Kumar, S.H.H, Bakshi, V. Antiulcer effect of formulation of Aloe Vera & Liquorice gaint aspirin induced peptic ulcer. International Journal of Applied Pharmaceutical Sciences and Research, 1(1): 42-45. 2016.