Oussou N’Guessan Jean-Baptiste^1,, Kouakou Kouakou Léandre¹, Ehile Ehilé Hervé¹, Yoboue Kouamé Landry¹, Yapo Angoué Paul¹, Ehile Ehouan Etienne¹

Macaranga barteri and Terminalia superba are two plants from the Ivorian flora used in traditional medicine to treat gastric disorders. This study aims to evaluate the cyprotective effect of a herbal formulation based on these herbs (AEMbTs) against induced ulcers in rats. The cytoprotective effect of AEMbTs was carried out on HCl/Ethanol and ibuprofen rats’ models. For each model, nine groups of six rats each were formed. The rats were orally pretreated with distilled water (Groups I and II), those of groups III and IV received Cimetidine (12 mg/kg bw) and Maalox (50 mg/kg bw) respectively in HCl/Ethanol model while Misoprostol (0,012 mg/kg bw) and Ranitidine (50 mg/kg bw) were respectively used as standard drugs in ibuprofen model. The rats in groups V to IX received AEMbTs at the respective doses of 31.25; 62.50; 125; 250 and 500 mg/kg bw. In the HCl/ethanol model, one hour after administration of the various treatments, all groups except group I received HCl/Ethanol orally at 1 ml/150 g bw. All the animals were sacrificed one hour later. As for the ibuprofen model, thirty (30) minutes after the treatments, all the animals except those of group 1 received orally a first dose of ibuprofen (500 mg/kg bw). Fifteen hours after the first dose, a second dose of ibuprofen was administered. Six (6) hours later, all the animals were sacrificed, the mucus was collected and weighed, the ulcerations were measured, the ulceration index and the inhibition percentage of the ulcerations were calculated. The results showed that the doses of AEMbTs induced significant decrease (p<0.05) of the ulceration areas. The inhibition values were 99.29 and 100%, respectively for the ibuprofen and HCl/ethanol models at the extract dose of 500 mg/kg bw. AEMbTs, also, significantly (p<0.001) increased mucus production. These results suggest that the herbal formulation has cytoprotective effects on the rat stomach and could justify the use of these plants by traditional healers to treat gastric disorders.

Macaranga barteri, Terminalia superba, HCl/Ethanol, ibuprofen, gastric ulcer