In vitro Anti-inflammatory and in vivo Immuno-modulatory Activities of Ethanolic Extract of Strychnos camptoneura (Loganiaceae) Seeds

Morabandza Cyr Jonas^{1, 2,} , Moulari Brice^{1, 3}, Gombé Assoungou Hermann^{1, 2} and Abena Ange Antoine^{1, 2}

¹Laboratory of Microbiology, infectiology and Immunology, higher normal school, Marien Ngouabi University B.P. 69, Brazzaville (Congo)

²Laboratory of Biochemistry and Pharmacology, Heath Sciences Faculty, Marien Ngouabi University B.P. 69, Brazzaville (Congo)

³Laboratory of Biochemistry and Pharmacology, Heath Sciences Faculty, Marien Ngouabi University B.P. 69, Brazzaville (Congo);PEPITE (EA 4267), University of Bourgogne / Franche-Comté, Besançon 25000, France

Cite this paper:
Morabandza Cyr Jonas, Moulari Brice, Gombé Assoungou Hermann and Abena Ange Antoine. In vitro Anti-inflammatory and in vivo Immuno-modulatory Activities of Ethanolic Extract of Strychnos camptoneura (Loganiaceae) Seeds. American Journal of Pharmacological Sciences. 2022; 10(1):38-46. doi: 10.12691/ajps-10-1-7

Abstract

Strychnos camptoneura is an essential ethnomedicinal plant of the Congolese traditional medicine known to prevent, mitigate or treat the skin diseases. We aimed to investigate the in vitro anti-inflammatory effects of the S. camptoneura seeds ethanolic extract (EE), using the human keratinocytes and dermal fibroblast cells, and the in vivo immuno-modulatory effects. The in vitro anti-inflammatory activity was evaluated using the lipopolysaccharide-induced inflammation model. For the in vivo immuno-modulatory activities of ethanolic extract, immune cells were quantified by flow cytometer technique. The results showed that EE exhibited a higher anti-inflammatory activity compared to a non-treated group. The immuno-modulatory assay revealed that EE stimulate the immune cells (CD3, NK cells) and circulating anti-inflammatory cytokines (IL-10 and IL-4). These results could constitute a scientific basis for using S. camptoneura in traditional medicine.

Keywords:
Strychnos camptoneura cytotoxicity anti-inflammatory immuno-modulatory human cells immune cells

This work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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