In-vivo Anticonvulsant and In-vitro Antimycobacterial Activities of 6-Aryl Pyridazine-3(2H)-One Derivatives

Mohammad Asif^1, , Anita Singh² and Lakshmayya¹

¹Department of Pharmacy, GRD (P.G) Institute of Management & Technology, Dehradun, India

²Department of Pharmaceutical sciences, Kumaun University, Bheemtal, Nainital, India

Cite this paper:
Mohammad Asif, Anita Singh and Lakshmayya. In-vivo Anticonvulsant and In-vitro Antimycobacterial Activities of 6-Aryl Pyridazine-3(2H)-One Derivatives. American Journal of Pharmacological Sciences. 2014; 2(1):1-6. doi: 10.12691/ajps-2-1-1

Abstract

Some 6-aryl-4,5-dihydropyridazin-3(2H)-one compounds (2a-f) were synthesized and evaluated for their in-vivo anticonvulsant activities against maximal electro shock (MES) and isoniazid (INH) induced seizure methods at 50mg/kg dose level. Neurotoxicity of all compounds (2a-f) was also tested at 50, 100 and 200mg/kg dose level. The in-vitro antitubercular activity was evaluated against Mycobacterium tuberculosis H37Rv by using the Microplate Alamar Blue Assay (MABA) method. The result showed that all compounds (2a-f) showed significant anticonvulsant activity against both MES and INH induced convulsion methods. Among all compounds (2a-f), highest activity was exhibited by compound 2e against MES and compound 2b against INH-induced convulsion methods. In both methods, phenytion sodium (25mg/kg) and sodium valproate (50mg/kg) were used as reference drugs. All compounds did not showed any neurotoxicity up to 200mg/kg dose level. In antitubercular activity, minimum inhibitor concentration of compound 2e and 2f was 12.5μg/ml and other remaining compounds (2a-d) were showed 25μg/ml when compared with reference drugs Isoniazid (3.12μg/ml), Pyrizinamide (3.12μg/ml) and Streptomycin (6.25μg/ml).

Keywords:
anticonvulsant antituberculosis nephrotoxicity pyridazinone

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