Antibacterial Applications of Anatase TiO₂ Nanoparticle

Hani A. Alhadrami^{1, 2}, Aisha Baqasi¹, Javed Iqbal³, Raniyah A.M. Shoudri¹, Ahmad Mohammad Ashshi⁴, Esam I. Azhar^{1, 5}, Faten Al-Hazmi⁶, Ahmed Al-Ghamdi⁶ and S. Wageh^{6, 7,}

¹Faculty of Applied Medical Sciences, Department of Medical Laboratory Technology, King Abdulaziz University, P. O. Box 80402 Jeddah 21589, Saudi Arabia

²Special Infectious Agents Unit, King Fahd Medical Research Centre, King Abdulaziz University, P. O. Box 80402 Jeddah 21589, Saudi Arabia;Center of Innovation in Personalized Medicine, King Fahd Medical Research Center, King Abdulaziz University, P. O. Box 80402, Jeddah, 21589, Kingdom of Saudi Arabia

³Center of Nanotechnology, King Abdulaziz University, Jeddah 21589, Saudia Arabia

⁴Department of Laboratory Medicine, Faculty of Applied Medical Sciences, Umm Al-Qura University, P.O. Box 7607, Makkah, Saudi Arabia

⁵Special Infectious Agents Unit, King Fahd Medical Research Centre, King Abdulaziz University, P. O. Box 80402 Jeddah 21589, Saudi Arabia

⁶Department of Physics, Faculty of Science, King Abdulaziz University, Jeddah, 21589, Saudi Arabia

⁷Physics and Engineering Mathematics Department, Faculty of Electronic Engineering, Menoufia University, Menouf, 32952, Egypt

Cite this paper:
Hani A. Alhadrami, Aisha Baqasi, Javed Iqbal, Raniyah A.M. Shoudri, Ahmad Mohammad Ashshi, Esam I. Azhar, Faten Al-Hazmi, Ahmed Al-Ghamdi and S. Wageh. Antibacterial Applications of Anatase TiO₂ Nanoparticle. American Journal of Nanomaterials. 2017; 5(1):31-42. doi: 10.12691/ajn-5-1-5

Abstract

Many methods have been used for the preparation of nanostructured metal oxides. Here we report the synthesis of TiO₂ nanoparticles by facile hydrothermal process by varying the concentration of the precursor and reaction temperature while keeping the process time constant. Morphological, structural and optical studies were carried out by scanning electron microscopy equipped with energy dispersive spectroscopy, X-ray powder diffraction spectroscopy and VU-Vis-NIR spectroscopy. Morphological and compositional analysis reveal that the prepared nanoparticles are highly pure with an approximate average particle size 5-15nm. XRD studies showed their crystalline structure and the sizes around 5 nm, while the optical absorption studies in the photon wavelength range 300-600 nm reveal that the strong absorbance peak is positioned at around 3.5 eV nm whereas visible energy is almost transparent for the materials. Finally, the antibacterial effect of TiO₂ nanoparticles has been studied. Plating technique was used to determine lowest concentration that prevent or inhibit growth of bacteria. This technique of TiO₂ nanoparticle was used against most common organisms which cause wound infection including: MRSA, E. coli and Pseudomonas aeruginosa. Different concentrations of TiO₂ nanoparticles were used 100 μg/ml, 200 μg/ml, 400 μg/ml, 600 μg/ml and 800 μg/ml. Inhibition of bacteria was different for prepared TiO₂ samples due to different concentration of the precursors and synthesis temperature.

Keywords:
TiO₂ nanoparticles crystal structure antibacterial

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