American Journal of Food Science and Technology
ISSN (Print): 2333-4827 ISSN (Online): 2333-4835 Website: https://www.sciepub.com/journal/ajfst Editor-in-chief: Hyo Choi
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American Journal of Food Science and Technology. 2020, 8(3), 87-98
DOI: 10.12691/ajfst-8-3-2
Open AccessArticle

HPLC Determination of the Gallic Acid and Chebulinic Acid Contents of Phyllanthus emblica Linn., Terminalia bellirica Roxb., Terminalia chebula Retz. and Triphala Products from Chae Son district, Lampang, Thailand

Wilart Pompimon1, Saranya Wattananon2, Phansuang Udomputtimekakul1, , Wipanoot Baison1, Punchavee Sombutsiri1, Angkhana Chuajedton3 and Boonthawan Wingwon4

1Department of Chemistry, Faculty of Science, Lampang Rajabhat University, 52100, Lampang, Thailand

2Department of Food Science, Faculty of Agricultural Technology, Lampang Rajabhat University, 52100, Lampang, Thailand

3Department of Biology, Faculty of Science, Lampang Rajabhat University, 52100, Lampang, Thailand

4Department of Management Science, Faculty of Management Science, Lampang Rajabhat University, 52100, Lampang, Thailand

Pub. Date: April 27, 2020

Cite this paper:
Wilart Pompimon, Saranya Wattananon, Phansuang Udomputtimekakul, Wipanoot Baison, Punchavee Sombutsiri, Angkhana Chuajedton and Boonthawan Wingwon. HPLC Determination of the Gallic Acid and Chebulinic Acid Contents of Phyllanthus emblica Linn., Terminalia bellirica Roxb., Terminalia chebula Retz. and Triphala Products from Chae Son district, Lampang, Thailand. American Journal of Food Science and Technology. 2020; 8(3):87-98. doi: 10.12691/ajfst-8-3-2

Abstract

The gallic acid and chebulinic acid contents in Phyllanthus emblica Linn., Terminalia bellirica Roxb., Terminalia chebula Retz. and Triphala products from Chae Son district, Lampang, Thailand at 2017 and 2018 seasons were studied. The HPLC separation was validated for analysis the hexane, ethyl acetate and methanol herb extracts. The gallic acid contents in the methanol extracts of P. emblica and T. chebula collected in 2018 were amounted to be double those of the same plants collected in 2017. The chebulinic content in the methanol extract of P. emblica collected in 2018 was almost double that of the same plant collected in 2017. The gallic acid contents of two Triphala products (with sugar and sugar-free) were not found to be different while the chebulinic contents of the two Triphala products for 2018 were 50% higher than those of 2017. The gallic acid and chebulinic acid contents variation form year to year should be taken into consideration for the production of Triphala products.

Keywords:
Triphala gallic acid chebulinic acid Phyllanthus emblica Terminalia bellirica Terminalia chebula

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References:

[1]  Gowda, D.V., Muguli, G., Rangesh, P.R. and Deshpande, R.D. Phytochemical and pharmacological actions of triphala: Ayurvedic formulation-a review. Int J Pharm Sci Rev Res, 15(2) n12. 61-65. 2012.
 
[2]  Kumar, N.S., Nair, A.S., Nair, A.M. and Murali, M. Pharmacological and Therapeutic effects of triphala-A literature review. J Pharmacogn Phytochem, 5(3). 23-27. 2016.
 
[3]  Kondawar, M.S., Kamble, K.G. and Mali, D.S. Quantitative estimation of gallic acid and ascorbic acid in a marketed herbal medicine: Triphala churna by high performance thin layer chromatography. Int J PharmTech Res, 3(3). 1593-1599. 2011.
 
[4]  Saraphanchotiwitthaya, A. and Sripalakit, P. Immunomodulatory effect of different proportions of the herbal mixture in triphala on human T lymphocytes (Molt-4). Int J Pharm Pharm Sci, 7(7). 282-288. 2015.
 
[5]  Sato, V.H., Sungthong, B., Nuamnaichati, N., Rinthong, .P., Mangmool, S. and Sato, H. In vivo and In vitro evidence for the antihyperuricemic, anti-inflammatory and antioxidant effects of a traditional Ayurvedic medicine, Triphala. Nat Prod Commun, 12(10). 1635-1638. 2017.
 
[6]  Pathompak, P., Charoenchai, L. and Monton, C. The cholesterol esterase inhibition and total phenolic content of aqueous extract of triphala of and modified triphala formulas. Bulletin of Health, Science and Technology, 13(2). 25-30. 2015.
 
[7]  Bureau of Drug and Narcotic Department of Medical Sciences, Thai herbal pharmacopoeia 2016, The Agricultural Co-operative federation of Thailand., Ltd, Bangkok, 2016, 262-269, 357-375.
 
[8]  Charoenchai, L., Pathompak, P., Madaka, F., Settharaksa, S. and Saingam, W. HPLC–MS profiles and quantitative analysis of triphala formulation. Bulletin of Health, Science and Technology, 14(1). 57-67. 2016.
 
[9]  Gaire, B.P. and Subedi, L. Phytochemistry, pharmacology and medicinal properties of Phyllanthus emblica Linn. Chin J Integr Med, 1-8. 2014.
 
[10]  Gupta, A., Kumar, R., Kumar, S. and Pandey, A.K. Molecular Biology and Pharmacognosy of Beneficial Plants (Lenin Media Private Limited, Delhi, India), 2017, 52-64.
 
[11]  Gupta, P.C. Biological and pharmacological properties of Terminalia chebula Retz (HARITAKI)-an overview. Int J PharmPharm Sci, 4(3). 62-68. 2012.
 
[12]  Vadde, R., Radhakrishnan, S., Reddivari, L. and Vanamala, J.K.P. Triphala extract suppresses proliferation and induces apoptosis in human colon cancer stem cell via suppressing c-Myc/Cyclin D1 and elevation of Bax/Bcl-2 ratio. BioMed Research International, 1-12. 2015.
 
[13]  Parveen, R., Shamsi, T.N., Singh, G., Athar, T. and Fatima, S. Phytochemical analysis and In-vitro biochemical characterization of aqueous and methanolic extract of Triphala, a conventional herbal remedy. Biotechnol Rep, 17. 126-136. 2018.
 
[14]  Kirubanandan, S. and Renganathan, S. Evaluation of antimicrobial potential of aqueous and alcoholic extract of Triphala against wound pathogens. J Med Plants Stud, 3(6). 56-59. 2015.
 
[15]  Prabu, D., Kirubanandan, S., Ponnudurai, K., Nappinnai, M., Jinu, A.J.S. and Renganathan, S. Anti-inflammatory and analgesic activities of methanol extract of Triphala-a poly herbal formulation, Orient Pharm Exp Med, 8(4). 423-429. 2008.