Calcium Exchange Rates in Rat Kidney Tubule Cells Affected by Insulin and pH

B.W. Brazier^1,

¹Bendigo Kangan Institute, Bendigo, Victoria, Australia

Cite this paper:
B.W. Brazier. Calcium Exchange Rates in Rat Kidney Tubule Cells Affected by Insulin and pH. American Journal of Food and Nutrition. 2016; 4(5):115-126. doi: 10.12691/ajfn-4-5-1

Abstract

Calcium exchange rates in rat kidney tubule are measured by measuring the loss of Ca⁴⁵ isotopes from tubule cells when the tubules are placed in Ca⁴⁵ free media that was adjusted to different pH sand when different additives of sulphate, acetate, glutamate and /or insulin were included. The exchange rates showed decrease with low pH and glutamine but increased with addition of acetate and insulin. The effect of insulin is considered in relation to its observed effect on protein induced postprandial calciuria.

Keywords:
hypercalciuria insulin calcium exchange rates gluconeogenesis ammoniogenisis

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References:

[1]	Brazier BW (2016a)Hypercalciuriaeffect of high protein diets, American Journal Nutrition and Food Science, Vol. 4, No. 1, 20-29.
[2]	Brazier BW (2016b) ‘Effect of the level of dietary fat and fat type on postprandial calciuria and of insulin’ American Journal Nutrition and Food Science’, Vol. 4, No. 2, 55-62
[3]	Allen, L.H., Block, G.D., Wood, Rl. and Bryce, G.F. (1981), 'The role of insulin and parathyroid hormone in the protein induced calciuria of man', Nut.Res., 1981, 1: 3-11.
[4]	Wood, RJ. and Allen, L.H. (1983) 'Evidence for insulin involvement in arginine and glucose- induced hypercalciuria in the rat', J.Nutr., 113(8): 1561-67.
[5]	Howe, J.C. (1990) 'Postprandial response of calcium metabolism in postmenopausal women to meals varying in protein level/source', Metabolism, 39(12): 1246-52.
[6]	Brazier BW. (2016).’Effect of serum insulin on calciuria following protein meals in humansAmerican Journal Nutrition and Food Science. 2016, Vol. 4, No. 3, 63-67.
[7]	Wachman, A and Bernstein, D.S. (1960) 'Diet and osteoporosis', Lancet, 1: 958-59.
[8]	Walser, M. and Browden, A (1959) 'Ion association II the effect of sulfate infusion on calcium excretion', J.Clin.Invest., 38: 1404-10
[9]	Whiting, S.I.and Draper, H.H. (1981) 'Diet acidity and bone composition', J.Nut., 111: 1720- 26.
[10]	Zemel, M.B., Schuette, S.A, Hegsted, M. and Linkswiler, H.M. (1981) 'Role of the sulfurcontaining amino acids in protein-induced hypercalciuria in men', J.Nut., 111(3): 545-52.
[11]	Lemann, J., Litzow, J.R and Lennon, E.J. (1967) The effect of treatment of acidosis on calcium balance in patients with chronic azotemic renal disease, In.Clin.Invest., 46(2) 280-6.
[12]	Studer, RK. and Borle, AB. (1979) 'Effect of pH on the calcium metabolism of isolated rat kidney cells', J.Membrane BioI., 48(4): 325-4.
[13]	Leidid-Buckne,G and Ziegler, R (2001) ‘Diabetes mellitus a risk for osteoporosis?’. Experimental and Clinical Endocrinology & Diabetes 109 (suppl 2): 493-514.
[14]	Osteoporosis Australia (2014),’Diabetes and osteoporosis Consumer Factsheet’, 1st Edition 08/14.
[15]	Silva, P., Ross, B. and Spokes, K. (1980) 'Competition between sodium reabsorption and gluconeogensis in kidneys of steroid-treated rats', Am.J.Physiol., 238(4): F290-5.
[16]	Janda, s. (1969) 'Mechanism of calcium transport in kidney cortex slices', Physiologia Bohemoslovaca, 18(5): 413-23.
[17]	Mahler, RJ. andSzabo, O. (1967) 'Insulin action upon insulin-insensitive tissue following impaired degradation', Proc.Soc.Exp.Biol.Med., 125: 879-82.
[18]	Uchikawa, T.and Borle, AB. (1978) 'Kinetic analysis of calcium desaturation curves from isolated kidney cells.', Am.J.Physiol., 234(1): R29-R338.
[19]	Price, RG. (1979) Isolation of Kidney glomerculi and tubular fragments and cell populations, In: Reid, E., Cell Populations Methodological Surveys (B) Biochemistry: Vo1.9, Ellis Horwood, Chichester.
[20]	Borle, AB. (1975) 'Methods of Assessing Hormone Effects on Calcium Fluxes in vitro, in Methods' in Enzymology, Hormone Action, Part D, J.G. Hardman and B.W. O'Malley (eds.) New York, Academic Press, vo1.39, pp.513-573.
[21]	Elaine M. Worcester, and Fredric L. Coe, (2008),‘New Insights into the Pathogenesis of Idiopathic Hypercalciuria’. SeminNephrol. Mar; 28(2): 120-132
[22]	Brazier BW, (2016c) ‘Calcium exchange rate data’ http://barry-bwb2.tripod.com/calcium_exchange.html
[23]	Brosnan, J.T., McPhee, P., Hall, B. and Parry, D.M. (1978) 'Renal glutamine metabolism in rats fed high protein diets', Am.J.Phvsiol., 235 (3), pp.E261-5.
[24]	Nissim, 1., Nissim, I. and Yudkoff, M. (1991) 'Adaptation of renal tricarboxylic acid cycle metabolism to various acid-base states: study with [3-13C, 5-15N] glutamine', Miner- Electrolvte-Metab., 17(1): 21-31.
[25]	Nissim, 1., Nissim, I. and Yudkoff, M. (1990) 'Carbon flux through tricarboxylic acid cycle in rat renal tubules', Biochem. Biophysic.Acta., 26: 1033(2): 194-200.
[26]	Tannen, RL. (1978) 'Ammonia metabolism', Am.J.Physiol., 235(4): F265-77.