Bioactive Components and Antioxidant Properties of Acacia nilotica Seeds: Exploring Their Potential as a Cholesterol-Lowering Agent in Rats

Yousif A. Elhassaneen^1, , Basma A. El-khateeb¹ and Esraa S. Abd El-Aty¹

¹Department of Nutrition and Food science, Faculty of Home Economics, Menoufia University, Shebin El-Kom, Egypt

Cite this paper:
Yousif A. Elhassaneen, Basma A. El-khateeb and Esraa S. Abd El-Aty. Bioactive Components and Antioxidant Properties of Acacia nilotica Seeds: Exploring Their Potential as a Cholesterol-Lowering Agent in Rats. American Journal of Food and Nutrition. 2025; 13(4):138-153. doi: 10.12691/ajfn-13-4-3

Abstract

This study examined the chemical composition, bioactive compounds, antioxidant activity of Acacia nilotica seed powder (ANS), and its effects on hypercholesterolemic rats over four weeks. Thirty-six rats were acclimated on a basal diet (BD) for two weeks, then divided into groups: a negative control on BD, a positive control fed a hypercholesterolemic diet (HCD) for 3 weeks, followed by BD, and three treatment groups receiving BD with 5%, 7%, and 9% ANS. Proximate analysis of ANS revealed 8.27% moisture, 9.43% crude fat, 27.13% protein, and 39.44% carbohydrates, indicating high nutritional value. Bioactive compounds included polysaccharides, polyphenols, saponins, alkaloids, flavonoids, and kaempferol, with low oxalates and tannins within safe limits. Antioxidant activity was 66.89%, comparable to standard antioxidants. ANS treatment improved metabolic parameters dose-dependently. Body weight gain (BWG), reduced by ~40% in untreated rats, improved by 10%, 20%, and 43% at 3, 6, and 9 g/100 g ANS, respectively. Feed intake and feed efficiency ratio similarly recovered. Liver enzymes AST, ALT, and ALP, elevated in the model by 39.8%, 61.8%, and 16.6%, decreased significantly with 9 g ANS by 22.1%, 95.5%, and 10.7%. Serum triglycerides and total cholesterol, increased by ~181% and 38%, declined dose-dependently with ANS, with TG dropping by ~260% and TC by ~22.7% at 9 g. HDL cholesterol rose by 25%, 37%, and 72%, while LDL and VLDL cholesterol fell by up to 44% and 41%. Glutathione levels (GSH and GSSG) improved, reversing 23.14% and 19.84% declines in controls. Erythrocyte antioxidant enzymes (GSH-Px, GSH-Rd, SOD, CAT) decreased by 25–38% in controls but increased by 23–45% with ANS. Oxidative stress markers ROS and MDA, elevated by 45% and 62.48%, were reduced dose-dependently, with MDA decreasing by 42.89% at the highest dose. These results demonstrate ANS’s potent antioxidant and lipid-lowering effects in hypercholesterolemic rats.

Keywords:
Hypercholesterolemia liver functions serum lipid profile antioxidant enzymes oxidative stress

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References:

[1]	Durrington, P. (2003). Dyslipidaemia. The Lancet, 362(9385), 717-731.‏
[2]	Biggerstaff, K. D., & Wooten, J. S. (2004). Understanding lipoproteins as transporters of cholesterol and other lipids. Advances in physiology education, 28(3), 105-106.‏
[3]	Carmena, R., Duriez, P., & Fruchart, J. C. (2004). Atherogenic lipoprotein particles in atherosclerosis. Circulation, 109 (Suppl-1), III-2.‏
[4]	Kontush, A., & Chapman, M. J. (2006). Antiatherogenic small, dense HDL—guardian angel of the arterial wall?. Nature clinical practice Cardiovascular medicine, 3(3), 144-153.‏
[5]	Hooper, D. U., Adair, E. C., Cardinale, B. J., Byrnes, J. E., Hungate, B. A., Matulich, K. L & O’Connor, M. I. (2012). A global synthesis reveals biodiversity loss as a major driver of ecosystem change. Nature, 486(7401), 105-108.
[6]	Ito, S., Shen, L., Dai, Q., Wu, S. C., Collins, L. B., Swenberg, J. A. & Zhang, Y. (2011). Tet proteins can convert 5-methylcytosine to 5-formylcytosine and 5-carboxylcytosine. Science, 333(6047),1300-1303.‏
[7]	Jouad, H., Haloui, M., Rhiouani, H., El Hilaly, J., & Eddouks, M. (2001). Ethnobotanical survey of medicinal plants used for the treatment of diabetes, cardiac and renal diseases in the North centre region of Morocco (Fez–Boulemane). Journal of ethnopharmacology, 77(2-3), 175-182.‏
[8]	Batiha, G. E., Akhtar, N., Alsayegh, A. A., Abusudah, W. F., Almohmadi, N. H., Shaheen, H. M., Singh, T. G., & De Waard, M. (2022). Bioactive compounds, pharmacological actions, and pharmacokinetics of genus Acacia. Molecules, 27(21), 7340.
[9]	Ndamitso, M. M., Mustapha, S., Etsuyankpa, M. B., Ajai, A. I., & Mathew, J. T. (2017). Evaluation of chemical composition of Acacia nilotica seeds. FUW Trends in Science & Technology Journal, 2(2), 927-931.
[10]	Kumari, S., & Swer, T. L. (2025). Acacia nilotica Linn: a comprehensive review of its nutritional profile, pharmacological activities, and food applications. Phytochemistry Reviews, 1-25.
[11]	Abbasian, K., Asgarpanah, J., & Ziarati, P. (2015). Chemical composition profile of Acacia nilotica seed growing wild in the south of Iran. Oriental Journal of Chemistry, 31(2), 1027-1033.
[12]	Eleazu, C. O., Okafor, P. N., Amajor, J., Awa, E., Ikpeama, A. I., & Eleazu, K. C. (2011). Chemical composition, antioxidant activity, functional properties, and inhibitory action of unripe plantain (Musa paradisiaca) flour. African J. Biotech., 10(74), 16948-16952.
[13]	Babiker, E. E., Al-Juhaimi, F. Y., Alqah, H. A., Adisa, A. R., Adiamo, O. Q., Mohamed Ahmed, I. A., Alsawmahi, O. N., Ghafoor, K., Ozcan, M. M. (2019). The effect of Acacia nilotica seed extract on the physicochemical, microbiological, and oxidative stability of chicken patties. J. Food Sci. Technol., 56(8), 3910-3920.
[14]	Shalaby, O. A., Hassan, A. R., & Mehanna, E. T. (2024). Acacia nilotica as a potential remedy for obesity. Rec. Pharm. Biomed. Sci., 8(1), 142-146.
[15]	Gharib, M. A., Radwan, H. A., & Elhassaneen, Y. A. (2022). Nutrients and nutraceuticals content and in vitro biological activities of Reishi mushroom (Ganoderma lucidum) fruiting bodies. Alexandria Science Exchange Journal, 43(2), 301-316. https://doi.org/10.21608/ asejaiqjsae.2022.245271.
[16]	Singleton, V. L., & Rossi, J. A., Jr. (1965).Colorimetry of total phenolics with phosphomolybdic- phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16, 144-158.
[17]	Wolfe, K., Wu, X., & Liu, R. H. (2003). Antioxidant activity of apple peels. Journal of Agricultural and Food Chemistry, 51, 609–614.
[18]	Lichtenthaler, H. K. (1987). Chlorophylls and carotenoids, the pigments of photosynthetic biomembranes. In R. Douce & L. Packer (Eds.), Methods in Enzymology (pp. 350-382). Academic Press Inc., New York.
[19]	Zhishen, J., Mengcheng, T., & Jianming, W. (1999). The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry, 64, 555–559.
[20]	Vazirian, M., Dianat, S., Manayi, A., Ziari, R., Mousazadeh, A., Emran, H., Saeidnia, S. and Amanzadeh, Y. (2014). Anti-inflammatory effect, total polysaccharide, total phenolics content and antioxidant activity of the aqueous extract of three basidiomycetes. Research Journal of Pharmacognosy1:13-19.
[21]	Ghorai, N., Chakraborty, S., Guchhait, S., Saha, S., & Biswas, S. (2012). Estimation of total terpenoids concentration in plant tissues using a monoterpene, Linalool as standard reagent: Protocol Exchange. Protocol Exchange, 1-6. .
[22]	Schlemmer, U., Frølich, W., Prieto, R. M., & Grases, F. (2009). Phytate in foods and significance for humans: Food sources, intake, processing, bioavailability, protective role, and analysis. Molecular Nutrition & Food Research, 53(Suppl 2), S330–S375.
[23]	Van-Burden, T. P., & Robinson, W. C. (1981). Formation of complexes between protein and tannic acid. Journal of Agricultural and Food Chemistry, 1, 77.
[24]	Zhao, J., & Wang, M. Y. (2010). Colorimetric determination of total alkaloids in plant extracts. Journal of Analytical Methods in Chemistry, 2010, 482476.
[25]	Fenwick, D.E. and 0akenfuI1, D. (1981). Saponin content offood plants and some prepared foods. Journal of the Science of Food and Agriculture 34, 186-191.
[26]	Oke, O.L. (1966). Chemical Studies of Some Nigerian Vegetables. Experimental Agriculture, 1(2):125-129.
[27]	Fouda, W., Wael, M., Ibrahim, A., Ellamie1, M. and Gamal, R. (2019). Biochemical and mineral compositions of six brown seaweeds collected from Red Sea at Hurghada Coast. Indian Journal of Geo Marine Sciences. 48 (04), 484-491.
[28]	Marco, G. (1968). A rapid method for evaluation of antioxidants. Journal of the American Oil Chemists' Society, 45, 594-598.
[29]	Reeves, P., Nielsen, F. and Fahey, G. (1993). AIN-93 Purified Diets for Laboratory Rodents: Final Report of the American Institute of Nutrition Ad Hoc Writing Committee on the Reformulation of the AIN-76A Rodent Diet. Journal of Nutrition,123(11), 1939-1951.
[30]	Abd Elalal, N., El Seedy, G., & Elhassaneen, Y. (2021). Chemical composition, nutritional value, bioactive compounds content and biological activities of the brown alga (Sargassum subrepandum) collected from the Mediterranean Sea, Egypt. Alexandria Science Exchange Journal,42(4), 893-906. https://doi.org/10.21608/asejaiqjsae.2021.205527.
[31]	NRC, National Research Council (1996). Guide for the Care and Use of Laboratory Animals. Washington: National Academy Press.
[32]	Chapman, D.G., Castilla, R. and Champbell, J.A. (1959). "Evaluation of protein in food. I.A. Method for the determination of protein efficiency ratio". Can. J. Biochemistry Physiology, 37, 679-686.
[33]	Stroev, S. & Makarova, M. (1989). "Textbook of clinical chemistry", Carl A. Burtis, 3rd ed., WB Saunders, Philadelphia, USA.
[34]	Yound, D. S. (1975). Determination of GOT. Clin. Chem., 22 (5): 21-27.
[35]	Tietz, N.W. (1976). "Fundamental of Clinical Chemistry". Philadelphia, W.B. Saunders, P. 243.
[36]	Vassault, A., Grafmeyer, D., Graeve, J., Cohen, R., Beaudonnet, A. and Bienvenu, J. (1999). Quality specifications and allowable standards for validation of methods used in clinical biochemistry. Ann Biol Clin (Paris)., 57(6): 685-95.
[37]	Fossati, P. and Prencipe, L. (1982). Serum triglycerides determined colorimetrically with an enzyme that produces hydrogen peroxide. Clin. Chem., 28, 2077–2080.
[38]	Richmond, W. (1973). Preparation and properties of a cholesterol oxidase from Nocardia sp. and its application to the enzymatic assay of total cholesterol in serum. Clinical Chemistry, 19,1350-1356.
[39]	Lopes-Virella, M.F., Stone, P., Ellis, S. and Colwell, J.A. (1977). Cholesterol determination in high-density lipoproteins separated by three different methods. Clin Chem. 23(5):882-884.
[40]	Islam, S.M.T., Osa-Andrews, B., Jones, P.M., Muthukumar, A.R., Hashim, I. Cao, J. (2022). Methods of Low-Density Lipoprotein-Cholesterol Measurement: Analytical and Clinical Applications. 33(4): 282-294.
[41]	Ellman, G. L. (1959). Tissue sulfhydryl groups. Archives of Biochemistry and Biophysics, 82(1), 70-77.
[42]	Splittgerber, A. G., & Tappel, A. L. (1979). Inhibition of glutathione peroxidase by cadmium and other metal ions. Archives of biochemistry and biophysics, 197(2), 534-542.‏
[43]	Aebi, H. (1974). Catalase. In Methods of enzymatic analysis (pp. 673-684). Academic press.
[44]	Mett, J., & Müller, U. (2021). The medium-chain fatty acid decanoic acid reduces oxidative stress levels in neuroblastoma cells. Scientific Reports, 11(1), 6135.
[45]	Buege, J. A., & Aust, S. D. (1978). Microsomal lipid peroxidation. In Methods in enzymology,. 52, 302-310).
[46]	Jambunathan, N. (2010). Determination and detection of reactive oxygen species (ROS), lipid peroxidation, and electrolyte leakage in plants. In Methods in Molecular Biology, 639, 292-298.
[47]	Snedecor, G.W. and Cochran, W.G. (1967). Statistical Methods, Sixth Edition. Lowa State University Press, Ames, IA.
[48]	Bwai, M. D., Uzama, D., Abubakar, S., Olajide, O. O., Ikokoh, P. P., & Magu, J. (2015). Proximate, elemental, phytochemical, and anti-fungal analysis of Acacia nilotica fruit. Pharmac. & Bio. Evaluations, 2(3), 52-59.
[49]	Adamu, H. M., Ushie, O. A., & Elisha, B. (2013). Chemical and nutrient analysis of raw and fermented seeds of Cassia tora. J. Physical Sci. & Innov., 5(1), 125-138.
[50]	Elemo, G. N., Babajide, O., Elemo, O., & Ochuko, L. E. (2011). Comprehensive investigation into the nutritional composition of dehulled and defatted African locust bean seed (Parkia biglobosa). Afr. J. Plants Sci., 5, 291-295.
[51]	Abdelrahman, S. M. E., Osman, A., & Haron, R. (2010). The chemical composition of pigeon pea (Cajanus cajan) seed and functional properties of protein isolate. Pak. J. Nutr., 9, 1069-1073.
[52]	Abubakar, S., Godwin, H., Ogbadu, P., Onyenekwe, C., Olorode, O., & Rebecca, W. N. (2014). Evaluating the nutritional potential of Acacia sieberiana seeds (DC) growing in the northwest of Nigeria. J. Bio & Life Sci., 5(2), 25-36.
[53]	Helen, F. (2003). Brown marine algae: A survey of therapeutic potentials. Alternative & complementary therapies. February, PP: 29-33.
[54]	Bixler, H. and Porse, A. (2011). Decade of change in the seaweed hydrocolloids industry, J. Appl. Phycol., 23, 321-335.
[55]	Fitton, J. H., Irhimeh, M., & Teas, J. (2008). Marine algae and polysaccharides with therapeutic applications. Marine nutraceuticals and functional foods, 345.
[56]	Nagaoka, M., Shibata, H., Kimura-Takagi, I., Hashimoto, S., Aiyama, R., Ueyama, S. and Yokokura, T. (2000). Anti-ulcer effects and biological activities of polysaccharides from marine algae. Biofactors.12(1-4), 267-74.
[57]	El-Gamal, N. T. (2020). Studies on the antioxidant activities of brown algae and their effects on obesity and osteoporosis in rats. Ph.D. Thesis in Nutrition and Food Science, Faculty of Home Economics, Menoufia University, Shebin El-Kom, Egypt.
[58]	Elhassaneen, Y., Ragab, S. and Essa, E. (2020). Chemical and nutritional studies on extracts of food processing by-products and their effects on obesity complications in rats. Journal of Home Economics, 30 (2): 1-26. DOI: 10.21608/mkas.2020.156506.
[59]	Elhassaneen, Y., Abd El-Rahman, A. and El-Samouny, S. (2021-a). Potential Protective Effects of Cauliflower Leaves and Prickly Pear Fruits Skin on Liver Disorders Induced by Carbon Tetrachloride in Rats. Journal of Home Economics, 32(1),19-42. [DOI: 110.21608/mkas.100636.1085].
[60]	Ito, K. and Tsuchida, Y. (1972). The effect of algal polysaccharides on depressing of plasma cholesterol level in rats. Proceedings of the7th International Seaweed Symposium, pp 451–455.
[61]	Burtin, P. (2003). Nutritional value of seaweeds. Electron. J. Environ. Agric. Food Chem. 2, 498–503.
[62]	Masao, T. (2008). Mechanism of calcium oxalate renal stone formation and renal tubular cell injury. International J. of Urology, 15 (2), 115-120.
[63]	Elhassaneen, Y.A. and Sanad, M.I. (2009). Phenolics, Selenium, Vitamin C, Amino Acids and Pungency Levels and Antioxidant Activities of Two Egyptian Onion Varieties. American J. of Food Technology, 4(6), 241-254.
[64]	Elhassaneen, Y., El-Waseef, S., Fathy, N. and Sarah, S. A. (2016). Bioactive Compounds and Antioxidant Potential of Food Industry By-products in Egypt. American Journal of Food and Nutrition, 4(1), 1-7.
[65]	Elhassaneen, Y., Mekawy, S., Khder, S., & Salman, M. (2019). Effect of some plant parts powder on obesity complications of obese rats. Journal of Home Economics, 29(1), 83-106. https://doi.org/10.21608/mkas.2017.166177.
[66]	Chung, K. T., Wong, T. Y., Wei, C. I., Huang, Y. W., & Lin, Y. (1998). Tannins and human health: A review. Critical Reviews in Food Science and Nutrition, 38(6), 421-464.
[67]	Lewu, M.N., Adebola, P.O. and Afolayan, A.J. (2010). Effect of cooking on the mineral contents and anti-nutritional factors in seven accessions of Colocasia esculenta (L.) Schott growing in South Africa, J. Food Comp. Anal., 23, 389–393.
[68]	Oboh, G. and Akindahunsi, A.A. (2003). Biochemical changes in cassava products (flour & gari) subjected to Saccharomyces cerevisae solid media fermentation, Food Chem. , 8, 599–602.
[69]	Allen, Y. C. and Yi, C. C. (2013). "A review of the dietary flavonoid, kaempferol on human health and cancer chemoprevention", Food Chem., 138(4), 2099–2107.
[70]	Jukanti, A.K.P.M., Gaur, C.L., Gowda, L. and Chibbar, R.N. (2012).Nutritional quality and health benefits of chickpea (Cicer arietinum L.): a review,Brit. J. Nutr., 108, 11–26.
[71]	Timilsena, Y. P., Phosanam, A., & Stockmann, R. (2023). Perspectives on saponins: food functionality and applications. International Journal of Molecular Sciences, 24(17), 13538.‏
[72]	Saini, M. L. (2008). Comparative pharmacognostical and antimicrobial studies of Acacia species (Mimosaceae). J. Medicinal Plants Res., 2(12), 378-386.
[73]	Ismail, A., Marjan, Z.M. and Foong, C.W. (2004). Total antioxidant activity and phenolic content in selected vegetables. Food Chem. 87(4):581–586.
[74]	Barros, L., Baptista, P., Correia, D.M., Morais, J.S. and Ferreira, I.C.F.R. (2007). Effects of conservation treatment and cooking on the chemical composition and antioxidant activity of Portuguese wild edible mushrooms. J. Agric. Food Chem., 55(12):4781-4788.
[75]	Elhassaneen, Y. A., Gadallah, H. M. and Nasef, A. Z. (2023). Brown Algae (Sargassum Subrepandum) from Egypt Exhibited High Nutritional Composition and Bioactive Constituent's Content: A Biological Application on Obesity and its Complications in Experimental Rats. Journal of Agriculture and Crops, 9 (4): 441-461. [DOI: 10.32861/jac.94.441.461].
[76]	Mahran, M. Z. and Elhassaneen, Y. A. (2023-b). A Study of the Physical, Chemical, Phytochemical and Nutritional Properties of Wild Silybum marianum L. Seeds Oil to Investigate Its Potential Use to Boost Edible Oil Self-Sufficiency in Egypt. Alexandria Science Exchange Journal, 44, (1): 81-91. [DOI: 10.21608/asejaiqjsae.2023.292950].
[77]	Halliwell, B. and Aruoma, O. I. (1991). DNA damage by oxygen derived species. Its mechanism and measurement in mammalian systems. FEBS Letters, 281, 9–19.
[78]	Yang, S., Madyastha, P., Bingel, S., Ries, W. and Key, L. (2001). A new superoxide-generating oxidase in murine osteoclasts. J Biol Chem., 276, 5452–8.
[79]	Salman, M. (2016). Immunomodulatory and mineral absorption improvement of some red mushroom ". M.Sc. Thesis in Nutrition and Food Science, Faculty of Home Economics, Minoufiya University, Egypt.
[80]	Mahran, M., Elbassyouny, G. and Elhassaneen, Y. (2018). Preventive effects of onion skin powder against hepatotoxicity in rats treated with benzo(a)pyrene. Proceeding of the Annual Conference (13th Arab; 10th International), 11-12 April, Faculty of Specific Education, Mansoura University, " Higher Education in Egypt and the Arab World in the Light of Sustainable Development Strategies", Mansoura, Egypt. [http://sefac.mans.edu. eg/English /mokatamar.htm].
[81]	Aly, A., Ghada, M., Elbassyouny, G. and Elhassaneen, Y. (2018). Studies on the antioxidant properties of vegetables processing by-products extract and their roles in the alleviation of health complications caused by diabetes in rats. Proceeding of the 1st International Conference of the Faculty of Specific Education, Kafrelsheikh University, “Specific Sciences, their Developmental Role and Challenges of Labor Market” October, Sharm ElSheikh, Egypt, PP 1-24, 24-27.
[82]	Mehram, E., Alaa, O., Aboraya, and Elhassaneen, Y.A. (2021-b). Potential Effects of Food Processing Byproducts on Neurological and Immunological Disorders of Obese Rats. Alexandria Science Exchange Journal, 42, (2): 509-522.
[83]	Mehanna, H., Taberna, M., Von Buchwald, C., Tous, S., Brooks, J., Mena, M., ... & Ärztin, L. (2023). Prognostic implications of p16 and HPV discordance in oropharyngeal cancer (HNCIG-EPIC-OPC): a multicentre, multinational, individual patient data analysis. The Lancet Oncology, 24(3), 239-251.
[84]	Adewale, L. A., & Alli, A. (2017). Evaluation of root extract of Acacia nilotica on hematological and lipid profile in rats. European Journal of Medicinal Plants, 17(4), 1-7.
[85]	Hussain, M. B. H., Ahmad, R. S., Arshad, M. U., Imran, A., & Imran, M. (2021). Hypocholesterolemic effect of Acacia and Citrus honeys on cholesterol induced Sprague Dawley rats. Sain Malaysiana, 50(10),3095-3106.
[86]	Khalaf, S. S., Shalaby, O. A., Hassan, A. R., El-Kherbetawy, M. K., & Mehanna, E. T. (2023). Acacia nilotica stem bark extract ameliorates obesity, hyperlipidemia, and insulin resistance in a rat model of high fat diet-induced obesity. Journal of Traditional and Complementary Medicine, 13(4), 397-407.
[87]	Al-Mustafa, Z. H., & Dafallah, A. A. (2000). A study on the toxicology of Acacia nilotica. American Journal of Chinese Medicine, 28(1), 123-129.
[88]	Amata, I.A. and Nwagu, K.M. (2013). Comparative evaluation of the nutrient profile of the seeds of four selected tropical plants and maize, Int. J. Appl. Biol. Pharm. Tech, 4, 200–204.
[89]	Oyedeji, O., Daw, C. S., Labbe, N., Ayers, P., & Abdoulmoumine, N. (2017). Kinetics of the release of elemental precursors of syngas and syngas contaminants during devolatilization of switchgrass. Bioresource Technology, 244, 525-533.
[90]	Abd-ElAziz, Y. E. (2024). Effect of Delonix regia seeds on liver injury induced by carbon tetrachloride in rats: Technological, chemical, biological, and histopathological studies (Ph.D. Thesis). Faculty of Home Economics, Menoufia University, Shebin El-Kom, Egypt.
[91]	Murugesan, S. K., Chandrasekaran, G., & Narayanan, K. (2013). Protective effect of Acacia nilotica (L.)‎ against acetaminophen-induced hepatocellular damage in Wistar rats. Advances in Pharmacological and Pharmaceutical Sciences, 2013, 1-9.
[92]	Abdel Razik, H. F., Enayat, A. O., El Toumy, S. A. A., & Wafaa, E. A. A. (2006). Evaluation of hepatoprotective activity of Acacia nilotica leaves on CCl₄ induced liver damage in rats. Planta Medica, 72, P_006.
[93]	Elhassaneen, Y., Ragab, S., Thoraya, M., Azza, E. & Abeer, A. (2012). Effect of Sweet Violet (Viola odorata L.) Blossoms Powder on Liver and Kidney Functions as well as Serum Lipid Peroxidation of Rats Treated with Carbon Tetrachloride. The Journal of American Science, 9 (5):88-95.
[94]	Badawy, N. M. (2021). "Antioxidant activities of Milk Thistle (Silybum marinum) and its effects on liver disorders induced by carbontetrachloride ". MSc. Thesis in Nutrition and Food Science, Faculty of Home Economics, Menoufia University, Shebin El-Kom, Egypt (April, 2021).
[95]	Fati, G. (2017). "The effect of some bakery products fortified with functional food consumption on rats suffered from liver cancer ". Ph.D. Thesis in Nutrition and Food Science, Faculty of Home Economics, Menoufia University, Egypt.
[96]	Arthur, F. K., Woode, E., Terlabi, E. and Larbie, C. (2012b). "Evaluation of hepatoprotetctive effect of aqueous extract of Annona muricata (Linn.) leaf against carbon tetrachloride and acetaminopheninduced liver damage". J. Nat. Pharm., 3, 25–30.
[97]	Coria-Tellez, A., Efigenia, M., Elhadi, M. and Obledo-Vazquez, E. (2018). Annona muricata: A comprehensive review on its traditional medicinal uses, phytochemicals, pharmacological activities, mechanisms of action and toxicity. Arabian Journal of Chemistry, 11, 662–691.
[98]	Elhassaneen, Y., Ghamry, H. and Lotfy, L. (2018). "Potential chemoprevention of liver disorders by dietary curcumin in rats Treated with Benzo(a)pyrene". Proceeding of the 1st Scientific International Conference of the Faculty of Specific Education, Minia University, “Specific Education, innovation and labor market” 16-17 Juli, Minia, Egypt.
[99]	Alli, L. A., Adesokan, A. A., Salawu, O. A., & Akanji, M. A. (2015). Toxicological studies of aqueous extract of Acacia nilotica root. Interdisciplinary Toxicology, 8(1), 48-54.
[100]	Adewale, A. L. (2017). Evaluation of root extract of Acacia nilotica on haematological and lipid profile in rats. European Journal of Medicinal Plants, 17(4), 1-7.
[101]	Abuelgassim, A. O. (2013). Effect of Acacia nilotica fruit extract on serum glucose and lipid concentrations in alloxan-induced diabetic rats. Pakistan Journal of Biological Sciences, 16(21), 1398-1402.
[102]	Hafez, L. O., Brito-Casillas, Y., Abdelmageed, N., Alemán-Cabrera, I. M., Morad, S. A. F., Abdel-Raheem, M. H., & Wägner, A. M. (2024). The Acacia (Vachellia nilotica L.) P.J.H. Hurter & Mabb.): Traditional uses and recent advances on its pharmacological attributes and potential activities. Nutrients, 16(24), 4278.
[103]	Usunobun, U., & Okolie, N. P. (2015). Phytochemical, trace and mineral composition of Vernonia amygdalina leaves. International Journal of Biological and Pharmaceutical Research, 6(5), 393-399.
[104]	McAnlis, G.T., McEneny, J., Pearce, J. and Young, I.S. (1999). Absorption and antioxidant effects of quercetin from onions, in man. Eur. J. Clin. Nutr, 53(2): 92-96.
[105]	Kaneko, T., Kajji, k. and Matsuo, M. (1994). Protection of linoleic acid hydroperoxide-induced cytotoxicity by phenolic antioxidants. Free Radical Biol. Med. 16:405-409.
[106]	Aviram, M., Rosenblat, M., Billecke, S., Erogul, J., Sorenson, R., Bisgaier, C., Newton, R. and LaDu, B. (1999). Human serum paraoxonase is inactivated by oxidized low density liporotein and preserved by antioxidants. Free Radical Biology and Medicine, 26(7/8), 892-904.
[107]	Boraey, R. A. (2023). " Study of The Biological Activities of Ashwagandha Extract and Its Effects on Neurological Complications in Obese Ratsrats " MSc. Thesis in Nutrition and Food Science, Faculty of Home Economics, Minoufiya University, Shebin El-Kom, Egypt.
[108]	Kuhlmann, M., Burkhardt, G., Horsch, E., Wagner, M. and Kohler, H. (1998). Inhibition of oxidant-induced lipid peroxidation in cultured renal tublar epithelial cells by quercetin. Free Rad. Res, 29, 451-460.
[109]	Correa-Gordillo, J., Ortiz, J., Sanchez-Mejıa, M. and Pachon, H. (2012). Actividad antioxidante en guanabana (Annona muricata L.) una revisio´ n bibliogra´ fica. Bol. Latinoam. Caribe Plant. Med. Aromat., 11, 111–126.
[110]	Kamata, H. & Hirata, H. (1999). Redox regulation of cellular signalling. Cellular Signalling, 11(1), 1-14.
[111]	Rauf, A., Ibrahim, M., Alomar, T. S., AlMasoud, N., Khalil, A. A., Khan, M., Khalid, A., Jan, M. S., Formanowicz, D. & Quradha, M. M. (2024). Hypoglycemic, anti-inflammatory, and neuroprotective potentials of crude methanolic extract from Acacia nilotica L. Results of an in vitro study. Food Science and Nutrition, 12(5), 3483-3491.
[112]	Elmongy, N. F., Hussein, I. A., Said Ahmed, W. M., & Shatla, I. M. (2022). Cardioprotective effect of Cinnamomum zeylanicum extract on rats fed a high fat high fructose diet. Bulletin of the Egyptian Society of Physiological Sciences, 42(4), 344-358.
[113]	Rathod, N. B., Elabed, N., Punia, S., Ozogul, F., Kim, S.K. & Rocha, J. M. (2023). Recent developments in polyphenol applications on human health: A review with current knowledge. Plants, 12, 1217.
[114]	Sultana, B., Anwar, F. & Ashraf, M. (2009). Effect of extraction solvent/technique on the antioxidant activity of selected medicinal plant extracts. Molecules, 14, 2167-2180.