Purple Eggplant Infusion and Maceration As A Valuable Functional Food

Kane Affissata Fathim France^1, , Agbo Adouko Edith¹, Gbogbo Moussa² and Brou Kouakou¹

¹Nutrition and Food Security Laboratory, Food Science and Technology Department, Nangui Abrogoua University, 02 BP 801 Abidjan 02, Abidjan, Côte d’Ivoire

²Biochemistry and Microbiology Department, Jean Lorougnon Guede University, Daloa, Côte d'Ivoire

Cite this paper:
Kane Affissata Fathim France, Agbo Adouko Edith, Gbogbo Moussa and Brou Kouakou. Purple Eggplant Infusion and Maceration As A Valuable Functional Food. American Journal of Food and Nutrition. 2024; 12(6):152-158. doi: 10.12691/ajfn-12-6-1

Abstract

In Côte d'Ivoire, purple eggplant is commonly used in food preparation. It contains valuable nutrients and has antioxidant potential, offering benefits for metabolic diseases. However, the cooking process results in nutrient losses. To address this, infusion and maceration were proposed as alternative methods. This study aimed to evaluate the impact of eggplant maceration and infusion on glycemia and hypercholesterolemia. Physicochemical parameters, phytochemical compound content, and antioxidant activity were determined. The glycemic response of macerated and infused eggplants was assessed using the blood sugar response method, and lipid content was measured in Wistar rats. The results showed that macerated eggplant had higher protein (0.13 ± 0.02%), fat (0.06 ± 0.0%), fiber (0.08 ± 0.01%), and ash (0.03 ± 0.003%) contents, while the infusion was richer in total carbohydrates (0.29 ± 0.05%), total sugars (2.17 ± 0.06 mg/100g), and reducing sugars (0.88 ± 0.04 mg/100g). Phenolic compound content and antioxidant activity were higher in the infused eggplant compared to the macerated eggplant. The antioxidant inhibitory concentration of 50% (IC50) for DPPH activity was 0.05 mg/mL for infused eggplant, compared to 0.17 mg/mL for macerated eggplant. These IC50 values indicate strong antioxidant activity, with respective anti-radical powers of 2000.00 and 588.24 µmol of reduced DPPH/mg of sample. Normal glycemic values after the ingestion of macerated and infused eggplants were reached at 90 minutes, with 104.5 mg/dL and 94 mg/dL, respectively, and remained stable until the end of the experiment (240 minutes). Normal blood sugar levels range between 50.5 and 110.5 mg/dL. Both macerated and infused eggplants increased HDL cholesterol levels compared to the control. A significant difference (P<0.05) was observed between the macerated and infused eggplant samples for all parameters investigated. Based on phenolic compound content, antioxidant activity, the ability to decrease hyperglycemia, and increase HDL cholesterol, macerated and infused eggplants may be beneficial for the prevention of diabetes and hypercholesterolemia.

Keywords:
Macerate eggplant infusion eggplant diabetes hypercholesterolemia

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References:

[1]	Chioti, V., Zeliou, K., Bakogianni, A., Papaioannou, C., Biskinis, A., Petropoulos, C., Lamari, F. N., & Papasotiropoulos, V, Nutritional value of eggplant cultivars and association with sequence variation in genes coding for major phenolics, Plants, 11. 2267. 2022.
[2]	Quamruzzaman, A., Khatun, A., & Islam, F, Nutritional content and health benefits of Bangladeshi eggplant cultivars, European Journal of Agriculture and Food Sciences, 2 (4). 1–7. 2020.
[3]	Azizuddin, S., Muhammad Iqbal, M., & Qadeer, A., A review on therapeutic potential and nutritional composition of eggplant., European Academic Research, 10 (2). 545–557. 2020.
[4]	Guimarães, P. R., Galvão, A. M. P., Batista, C. M., Azevedo, G. S., Oliveira, R. D., Lamounier, R. P., Freire, N., Barros, A. M. D., Sakurai, E., Oliveira, J. P., Vieira, E. C., & Alvarez-Leite, J.I, Eggplant (Solanum melongena) infusion has a modest and transitory effect on hypercholesterolemic subjects, Brazilian Journal of Medical and Biological Research, 33. 1027–1036. 2000.
[5]	Agbo, A. E., Méité, S., Gbogouri, G. A., Gouekou, A. D., Kouamé, C. E., Kouassi, K., & Brou, K, Impact of antioxidant spices on vitamin B9 and β-carotene in steamed sweet potato leaves, Acta Horticulturae, 1292. 383–392. 2020.
[6]	Studzinska-Sroka, E., Galanty, A., Gosciniak, A., Wieczorek, M., Kłaput, M., Dudek-Makuch, M., & Cielecka-Piontek, J, Herbal infusions as a valuable functional food, Nutrients, 13. 4051. 2021.
[7]	Alves-Silva, J. M., Pedreiro, S., Cruz, M. T., Salgueiro, L., & Figueirinha, A, Exploring the traditional uses of Thymbra capitata infusion in Algarve (Portugal): Anti-inflammatory, wound healing, and anti-aging, Pharmaceuticals, 16. 1202. 2023.
[8]	AOAC, Official methods of analysis, 1990, 15th ed., 684.
[9]	Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A., & Smith, F, Colorimetric method for determination of sugars and related substances, Analytical Chemistry, 28. 350–356.1956.
[10]	Bernfeld, P, Amylase and proteases, In Methods in Enzymology Academic Press, New York, 149–158, 1955.
[11]	Kularatne, K. I. A., & de Freitas, C. R, Epiphytic lichens as biomonitors of airborne heavy metal pollution. Environmental and Experimental Botany, 88. 24–32. 2013.
[12]	Singleton, V. L., Orthofer, R., & Lamuela-Raventós, R. M, Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent, In Methods in Enzymology, Academic Press, 1999, Vol. 299, pp. 52–178.
[13]	Hariri, E. B., Sallé, G., & Andary, C, Involvement of flavonoids in the resistance of two poplar cultivars to mistletoe (Viscum album L.), Protoplasma, 162 (1). 20–26. 1991.
[14]	Heimler, D., Vignolini, P., Dini, M. G., Vincieri, F. F., & Romani, A, Anti-radical activity and polyphenol composition of local Brassicaceae edible varieties, Food Chemistry, 99 (3). 464–469. 2006.
[15]	Parejo, I., Codina, C., Petrakis, C., & Kefalas, P, Evaluation of scavenging activity assessed by Co (II)/EDTA-induced luminol chemiluminescence and DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical assay, Journal of Pharmacological and Toxicological Methods, 44. 507–512. 2000.
[16]	Kroyer, G. T, Red clover extract as antioxidant active and functional food ingredient. Innovative Food Science & Emerging Technologies, 5. 101–105. 2004.
[17]	Gharras, L., Hmamouchi, M., & Lamnouar, D, Comparative study of the hypoglycemic effect of six plants from the traditional Moroccan pharmacopoeia, Revue de Médecines et Pharmacopées Africaines, 1371–80. 1999.
[18]	Tietz, N. W, Clinical guide to laboratory tests, Philadelphia: WB Saunders, 1995, 3rd ed., pp. 268–273.
[19]	Prasain, J. K., Wang, C.-C., & Barnes, S, Mass spectrometric methods for the determination of flavonoids in biological samples, Free Radical Biology and Medicine, 31 (9). 1324–1350. 2004
[20]	Erdemgil, F. Z., Sanli, S., Sanli, N., Oezkan, G., Barbosa, J., Guiteras, J., & Beltran, J. Determination of pKa values of some hydroxylated benzoic acids in methanol-water binary mixtures by LC methodology and potentiometry, Talanta, 72 (2). 489–496. 2007.
[21]	Ponka, R., Nanka, E. L. T., Tambe, S. T., & Fokou, E, Composition nutritionnelle de quelques farines infantiles artisanales du Cameroun, International Journal of Innovation and Applied Studies, 16 (2). 250–292. 2016.
[22]	Itoua Okouango, Y. S., Elenga, M., Moutsamboté, J. M., Mananga, V., & Mbemba, F. Evaluation of the consumption and nutritional composition of Phytolacca dodecandra L’Herit leafy vegetables consumed by populations originating from the Owando and Makoua districts. Journal of Animal & Plant Sciences, 27. 4207–4218. 2015.
[23]	FAO, Table de composition des aliments d’Afrique de l’Ouest, Food and Agriculture Organization, Rome, 2012.
[24]	Lintas, C., & Cappelloni, M, Dietary fiber, resistant starch, and in vitro starch digestibility of cereal meals, Food Science and Nutrition, 42. 117–124. 1998.
[25]	Vodouhe, S., Dovoedo, A., Anihouvi, V. B., Tossou, R. C. R., & Soumanou, M. M, Influence of cooking method on the nutritional value of Solanum macrocarpum, Amaranthus hybridus, and Ocimum gratissimum, three traditional leafy vegetables acclimatized in Benin, International Journal of Biological and Chemical Sciences, 6 (5). 1926–1937. 2012.
[26]	Mackie, A., Balazs, B., & Neil, R, Roles for dietary fibre in the upper GI tract: The importance of viscosity, Food Research International, 88 (2). 34–38. 2016.
[27]	Ismail, A., Marjan, Z. M., & Foong, C. W, Total antioxidant activity and phenolic content in selected vegetables. Food Chemistry, 87. 581–586. 2004.
[28]	Seyar, R, Pharmacological characterization of eggplants and effects on selected biochemical parameters in diabetics (Master’s thesis). Faculty of Biological Sciences, Université Abdelhafid Boussouf Mila, Algeria, 2021.
[29]	Dranca, F., & Oroian, M, Optimization of ultrasound-assisted extraction of total monomeric anthocyanin (TMA) and total phenolic content (TPC) from eggplant (Solanum melongena L.) peel, Ultrasonics Sonochemistry, 32. 637–646. 2016.
[30]	Da Silva, E., Oliviera, A., & Lapa, A. Pharmacological evaluation of the anti-inflammatory activity of a citrus bioflavonoid, hesperidin and the isoflavonoids, duartin and claussequinone, in rats and mice. Journal of Pharmacology, 46 (2). 118–122. 1994.
[31]	Elekofehinti, O. O., Kamdem, J. P., Bolingon, A. A., Athayde, M. L., Lopes, S. R., Waczuk, E. P., Kade, I. J., Adanlawo, I. G., & Rocha, J. B. T. African eggplant (Solanum anguivi Lam.) fruit with bioactive polyphenolic compounds exerts in vitro antioxidant properties and inhibits Ca²⁺-induced mitochondrial swelling. Asian Pacific Journal of Tropical Biomedicine, 3. 757–766. 2013.
[32]	Traber, M. G., Buettner, G. R., & Bruno, R. S, The relationship between vitamin C, the gut-liver axis, and metabolic syndrome, Redox Biology, 21. 1–10. 2019.
[33]	Chang, C. L., & Wu, R. T. Quantification of (+)-catechin and (−)-epicatechin in coconut water by LC-MS. Food Chemistry, 126. 710–717. 2011.
[34]	Yong, J. W., Ge, L., Ng, Y. F., & Tan, S. N, The chemical composition and biological properties of coconut (Cocos nucifera L.) water, Molecules, 14 (5). 144–5164. 2009..
[35]	Halvorsen, B. L., Carlsen, M. H., Phillips, K. M., Bøhn, S. K., Holte, K., Jacobs Jr., D. R., & Blomhoff, R, Content of redox-active compounds (i.e., antioxidants) in foods consumed in the United States, American Journal of Clinical Nutrition, 84. 95–135. 2006.
[36]	Cai, M., Dou, B., Pugh, J. E., Lett, A. M., & Frost, G. S, The impact of starchy food structure on postprandial glycemic response and appetite: A systematic review with meta-analysis of randomized crossover trials, American Journal of Clinical Nutrition, 114. 472–487. 2021.
[37]	Naeem, M. Y., & Ugur, S, Nutritional content and health benefits of eggplant. Turkish, Journal of Agriculture and Food Science and Technology, 7. 31–36. 2019.
[38]	Basuny, A. M., Arafat, S. M., & El-Marzooq, M. A, Antioxidant and antihyperlipidemic activities of anthocyanins from eggplant peels, Journal of Pharma Research Review, 2. 50–57. 2012.