Applied Ecology and Environmental Sciences
ISSN (Print): 2328-3912 ISSN (Online): 2328-3920 Website: Editor-in-chief: Alejandro González Medina
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Applied Ecology and Environmental Sciences. 2020, 8(5), 273-277
DOI: 10.12691/aees-8-5-13
Open AccessArticle

Synthesis and Characterization of Mn3O4 Nanoparticles for Biological Studies

Murugan Perachiselvi1, Muthiah Sakthi Bagavathy1, J. Jenson Samraj1, E. Pushpalaksmi1 and G. Annadurai1,

1Division of Nanoscience, Sri Paramakalyani Centre for Excellence in Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi - 627412, India

Pub. Date: July 10, 2020

Cite this paper:
Murugan Perachiselvi, Muthiah Sakthi Bagavathy, J. Jenson Samraj, E. Pushpalaksmi and G. Annadurai. Synthesis and Characterization of Mn3O4 Nanoparticles for Biological Studies. Applied Ecology and Environmental Sciences. 2020; 8(5):273-277. doi: 10.12691/aees-8-5-13


Nowadays nanoparticles comprising the diversity of applications have been synthesized and used up in various fields. In our research, Manganese tetroxide NPs (Mn3O4) was synthesized by precipitation method. In the present investigation, antibacterial activity, as well as In-vitro cytotoxic effects of Mn3O4 NPs, have been evaluated. The cytotoxicity studies on the Vero cell line (African green monkey kidney cell line) were studied by using MTT assay at 72 hrs. The Cell viability which was observed during the process depends upon the time exposure and concentration. The antibacterial activity was evaluated against the bacteria such as Bacillus species, Escherichia coli, and Enterobacter sp. The synthesized nanoparticles were characterized using X-Ray Diffraction (XRD), Scanning Electron Microscope (SEM), Dynamic Light Scattering (DLS), and Fourier Transform Infra-Red Spectroscopy (FTIR). Moreover, In-vitro cytotoxic effects and the antibacterial activity of Mn3O4 NPs showed a better result at the concentration of 50 µg/100µl in Vero cell and the Zone of inhibition was measured for Enterobacter. This proves Mn3O4 NPs as promising biocompatible material.

Mn3O4 NPs cytotoxicity effects Vero cell and antibacterial activity

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