Murugan Perachiselvi¹, Muthiah Sakthi Bagavathy¹, J. Jenson Samraj¹, E. Pushpalaksmi¹, G. Annadurai^1,

Nowadays nanoparticles comprising the diversity of applications have been synthesized and used up in various fields. In our research, Manganese tetroxide NPs (Mn₃O₄) was synthesized by precipitation method. In the present investigation, antibacterial activity, as well as In-vitro cytotoxic effects of Mn₃O₄ NPs, have been evaluated. The cytotoxicity studies on the Vero cell line (African green monkey kidney cell line) were studied by using MTT assay at 72 hrs. The Cell viability which was observed during the process depends upon the time exposure and concentration. The antibacterial activity was evaluated against the bacteria such as Bacillus species, Escherichia coli, and Enterobacter sp. The synthesized nanoparticles were characterized using X-Ray Diffraction (XRD), Scanning Electron Microscope (SEM), Dynamic Light Scattering (DLS), and Fourier Transform Infra-Red Spectroscopy (FTIR). Moreover, In-vitro cytotoxic effects and the antibacterial activity of Mn₃O₄ NPs showed a better result at the concentration of 50 µg/100µl in Vero cell and the Zone of inhibition was measured for Enterobacter. This proves Mn₃O₄ NPs as promising biocompatible material.

Mn₃O₄ NPs, cytotoxicity effects, Vero cell, and antibacterial activity