1Division of Nanoscience, Sri Paramakalyani Centre for Excellence in Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi - 627412, India
Applied Ecology and Environmental Sciences.
2020,
Vol. 8 No. 5, 273-277
DOI: 10.12691/aees-8-5-13
Copyright © 2020 Science and Education PublishingCite this paper: Murugan Perachiselvi, Muthiah Sakthi Bagavathy, J. Jenson Samraj, E. Pushpalaksmi, G. Annadurai. Synthesis and Characterization of Mn
3O
4 Nanoparticles for Biological Studies.
Applied Ecology and Environmental Sciences. 2020; 8(5):273-277. doi: 10.12691/aees-8-5-13.
Correspondence to: G. Annadurai, Division of Nanoscience, Sri Paramakalyani Centre for Excellence in Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi - 627412, India. Email:
gannadurai@msuniv.ac.inAbstract
Nowadays nanoparticles comprising the diversity of applications have been synthesized and used up in various fields. In our research, Manganese tetroxide NPs (Mn3O4) was synthesized by precipitation method. In the present investigation, antibacterial activity, as well as In-vitro cytotoxic effects of Mn3O4 NPs, have been evaluated. The cytotoxicity studies on the Vero cell line (African green monkey kidney cell line) were studied by using MTT assay at 72 hrs. The Cell viability which was observed during the process depends upon the time exposure and concentration. The antibacterial activity was evaluated against the bacteria such as Bacillus species, Escherichia coli, and Enterobacter sp. The synthesized nanoparticles were characterized using X-Ray Diffraction (XRD), Scanning Electron Microscope (SEM), Dynamic Light Scattering (DLS), and Fourier Transform Infra-Red Spectroscopy (FTIR). Moreover, In-vitro cytotoxic effects and the antibacterial activity of Mn3O4 NPs showed a better result at the concentration of 50 µg/100µl in Vero cell and the Zone of inhibition was measured for Enterobacter. This proves Mn3O4 NPs as promising biocompatible material.
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