Protective Effect of Linseed Oil and Walnuts against Hypercholesterolemia Induced by Atherogenic Diet in Rats

Karima Said Mohamed Hammad¹, Saad Ahmed Hallabo¹ and Samy Mohamed Galal^1,

¹Department of Food Science, Faculty of Agriculture, Cairo University, Giza, Egypt

Cite this paper:
Karima Said Mohamed Hammad, Saad Ahmed Hallabo and Samy Mohamed Galal. Protective Effect of Linseed Oil and Walnuts against Hypercholesterolemia Induced by Atherogenic Diet in Rats. Journal of Food and Nutrition Research. 2015; 3(10):653-660. doi: 10.12691/jfnr-3-10-6

Abstract

Hypercholesterolemia is the most common pathologic process underlying atherosclerosis and cardiovascular disease. Alpha–linolenic acid (ALA) is believed to benefit the cardiovascular system. The objective of this study was to determine the effect of different levels of dietary ALA from linseed oil and walnut on serum lipid profile of rats fed on atherogenic diet (AD). Sixty male Sprague- Dawley rats weighed 121.3±10.6 g were divided into six groups. Rats were fed for 10 weeks on rodent diets contained 2% cholesterol , 3% corn oil and 20% fat whose source was from either palm stearin (saturated fat), corn oil, linseed oil or walnut at different levels of ALA that ranged from zero to 9.71%. Weight gain, liver weight, serum lipid profile and liver function parameters were determined. AD based on saturated fat resulted in significant alterations in serum lipids, increase in body weight gain and relative liver weight accompanied by negative effect on liver function parameters. Dietary ALA could counteract the detrimental effects brought about by the AD. The overall beneficial effects provided by high level of ALA were better than those given by low levels of ALA. Linseed oil exerted a hypolipidemic effect and could be considered as a promising functional food in cardiovascular disease.

Keywords:
alpha-linolenic acid linseed oil walnut hypercholesterolemia lipid profile liver function parameters

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References:

[1]	Priya, S., Yogesh,S. and Patil, U.K. Assessment of hypolipidemic potential of Chenopodium album linn on triton induced hyperlipidemic rats. Research Journal of Pharmacy and Technology. 3:187-192, 2010.
[2]	Anon. Hypercholesterolemia. University of Maryland Medical Center. http://umm.edu/health/medical/altmed/condition/hypercholesterolemia#ixzz3gkgP6zFc. (accessed 5/2015).
[3]	Third report of the National Cholesterol Education Program (NCEP) expert panel on detection, evaluation, and treatment of high blood cholesterol in adults (adult treatment panel iii) final report. Circulation.106:3143-3421, 2002.
[4]	Xu, J., Gao, H., Zhang, L., Chen, C., Yang, W., Deng, Q., Huang, Q. and Huang, F. A combination of flaxseed oil and astaxanthin alleviates atherosclerosis risk factors in high fat diet fed rats. Lipids in Health and Disease.13: 63, 2014.
[5]	Estadella, D., Oyama, L.M., Damaso, A.R., Ribeiro, E.B.and Oller Do Nascimento, C.M. Effect of palatable hyperlipidic diet on lipid metabolism of sedentary and exercised rats. Nutrition. 20: 218-224, 2004.
[6]	Jump, D.B., Depner,C.M. and Tripathy, S. Omega-3 fatty acid supplementation and cardiovascular disease. The Journal of Lipid Research. 53:2525-2545, 2012.
[7]	Popa, V-M, Gruia, A., Raba, D-N., Dumbrava, D.,Moldovan, C., Bordean, D.and Mateescu,C. Fatty acids composition and oil characteristics of linseed (LinumUsitatissimum L.) from Romania. Journal of Agroalimentary Processes and Technologies. 18:136-140, 2012.
[8]	Makni, M., Fetoui, H., Gargouri, N.K., Garoui, el M., Jaber, H., Makni, J., Boudawara, T. and Zeghal, N. Hypolipidemic and hepatoprotective effects of flax and pumpkin seed mixture rich in omega-3 and omega-6 fatty acids in hypercholesterolemic rats. Food Chemical Toxicology. 46:3714-3720, 2008.
[9]	Lemos, J.R.N., de Alencastro, M.G., Konrath, A.V., Cargnin,M. and Manfro, R.C. Flaxseed oil supplementation decreases C-reactive protein levels in chronic hemodialysis patients. Nutrition Research.32:921-927, 2012.
[10]	Gandev, S. Budding and grafting of the walnut (Juglansregia L.) and their effectiveness in Bulgaria. Bulgarian Journal of Agricultural Science. 13:683-689, 2007.
[11]	Aryapak,S. and Ziarati, P. Nutritive value of persian walnut (Juglansregia L.) orchards. American-Eurasian Journal of Agricultural and EnvironmentalSciences.14:1228-1235, 2014.
[12]	Amaral, J.S., Casal, S., Pereira, J.A., Seabra, R.M. and Oliveira, B.P. Determination of sterol and fatty acid compositions, oxidative stability, and nutritional value of six walnut (Juglans regia L) cultivars grown in Portugal. Journal of Agriculture and Food Chemistry.51:7698-7702, 2003.
[13]	Moigradean, D., Poiana, M.A., Alda, L-M. and Gogoasa, I.Quantitative identification of fatty acids from walnut and coconut oils using GC-MS method. Journal of Agroalimentary Processes and Technologies.19:459-463, 2013.
[14]	FDA. Food and Drug Administration. Qualified Health Claims: Letter of Enforcement Discretion—Walnuts and CoronaryHeart Disease. (Docket No 02P-0292) 2004.
[15]	Berryman, C.E., Grieger, J.A.,West, S.G., Chen, C.O., Blumberg, J.B., Rothblat, G.H., Sankaranarayanan, S. and Kris-Etherton, P.M. Acute consumption of walnuts and walnut components differentially affect postprandial lipemia, endothelial function, oxidative stress, and cholesterol efflux in humans with mild hypercholesterolemia. Journal ofNutrition.143:788-794, 2013.
[16]	Kris-Etherton, P.M. Walnuts decrease risk of cardiovascular disease: a summary of efficacy and biologic mechanisms. Journal of Nutrition. 144:547S-554S, 2014.
[17]	Vijaimohan, K., Jainu, M., Sabitha, K.E., Subramaniyam, S., Anandhan, C., Shyamala Devi, C.S. Beneficial effects of alpha linolenic acid rich flaxseed oil on growth performance and hepatic cholesterol metabolism in high fat diet fed rats.Life Sciences. 79:448-454, 2006.
[18]	Rizos, E.C., Ntzani, E.E., Bika, E., Kostapanos, M.S. and Elisaf, M.S. Association between omega-3 fatty acid supplementation and risk of major cardiovascular disease events a systematic review and meta-analysis. The Journal of the American Medical Association.308:1024-1033, 2012.
[19]	Vlachopoulos, C., Richter, D. and Stefanadis, C. Omega-3 fatty acid supplementation and cardiovascular disease events. The Journal of the American Medical Association. 309:27, 2013.
[20]	Adkins, Y. and Kelley, D.S. Mechanisms underlying the cardioprotective effects of omega-3 polyunsaturated fatty acids.The Journal of Nutritional Biochemistry. 21:781-792, 2010.
[21]	Dogan,M. and Akgulb, A. Fatty acid composition of some walnut (Juglansregia L.) cultivars from east Anatolia. Grasas y Aceites.56:328-331, 2005.
[22]	AOAC. Official Methods of Analysis of AOAC International. 19th Edition, Association of Official Analytical Chemists, Gaithersburg, Maryland, USA, 2012.
[23]	AOCS. Official Methods and Recommended Practices of the AOCS, 6th edition. Champaign, Illinois; AOCS press, 2009.
[24]	Morsi, M.K.S., Galal, S.M. and Elkholy, M. Synthesis and evaluation of sucrose esters.Egyptian Journal of Nutrition.23:1-23, 2008.
[25]	NRC. Guide for the Care and Use of Laboratory Animals. 8th edition. Washington, DC: National Academies Press, US, 1996.
[26]	Ridker, P.M., Stampfer. M.J. and Rifai, N. Novel risk factors for systemic atherosclerosis: acomparison of C-reactive protein, fibrinogen, homocysteine, lipoprotein (a), and standard cholesterol screening as predictors of peripheral arterial disease. The Journal of the American Medical Association. 285:2481-2485, 2001.
[27]	Brehm, A., Pfeiler, G., Pacini, G., Vierhapper, H. and Roden, M. Relationship between serum lipoprotein ratios and insulin resistance in obesity. Clinical Chemistry. 50:2316-2322, 2004.
[28]	Sambu, N.K., Kashinath, R.T. and Ambekar, J.G. Effect of diallyldisulphide on diabetes induced dyslipidemia in male albino rats. Journal of Clinical and Diagnostic Research. 9:BF01-BF03, 2015.
[29]	Go, R.E, Hwang, K.A., Kim, Y.S., Kim, S.H., Nam, K.H. and Choi, K.C. Effects of palm and sunflower oils on serum cholesterol and fatty liver in rats. Journal of MedicinalFood.18:363-369, 2015.
[30]	Codex Alimentarius Commission Codex Standard for Named Vegetable Oils, Codex Standard Series No. 210 - 2005, Rome, 2005.
[31]	Egyptian Organization for Stanardization and Quality. Egyptian Standard for vegetable edible oils. Part: 4. Edible linseed oil. ES: 49-4/2005.
[32]	Ozkan, G. and Koyuncu, M.A. Physical and chemical composition of some walnut (Juglans regia L) genotypes grown in Turkey. Grasas y Aceites.56:141-146, 2005.
[33]	Coelho, D.F., Pereira-Lancha, L.O., Chaves, D.S., Diwan, D., Ferraz, R., Campos-Ferraz, P.L., Poortmans, J.R., Lancha Junior, A.H. Effect of high-fat diets on body composition, lipid metabolism and insulin sensitivity, and the role of exercise on these parameters. Brazilian Journal of Medical and BiologicalResearch.44:966-972, 2011.
[34]	Timmers, S., de Vogel-van den Bosch, J., de Wit, N., Schaart, G., van Beurden, D., Hesselink, M., van der Meer, R. and Schrauwen, P. Differential effects of saturated versus unsaturated dietary fatty acids on weight gain and myocellular lipid profiles in mice. Nutrition and Diabetes.1:e11, 2011.
[35]	Sabate, J., Cordero-Macintyre, Z., Siapco, G., Torabian, S. and Haddad, E. Does regular walnut consumption lead to weight gain?. Bristish Journal of Nutrition. 94:859-864, 2005.
[36]	Almario, R.U., Vonghavaravat, V., Wong, R. and Kasim-Karakas, S.E. Effects of walnut consumption on plasma fatty acids and lipoproteins in combined hyperlipidemia. The American Journal of Clinical Nutrition. 74:72-9, 2001.
[37]	Stapleton, P.A., Goodwill, A.G., James, M.E., Brock, R.W. and Frisbee, J.C. Hypercholesterolemia and microvascular dysfunction: interventional strategies. Journal of Inflammation.7:54, 2010.
[38]	Kris-Etherton, P.M., Yu-Poth, S., Sabaté, J., Ratcliffe, H.E., Zhao, G. and Etherton, T.D. Nuts and their bioactive constituents: effects on serum lipids and other factors that affect disease risk. The American Journal of Clinical Nutrition.70:504S-11S, 1999.
[39]	Connor, W.E. Importance of n−3 fatty acids in health and disease. The American Journal of Clinical Nutrition. 71:171S-175S, 2000.
[40]	Dupasquier, C.M.C., Weber, A.M., Ander, B.P., Rampersad, P.P., Steigerwald, S., Wigle, J.T., Mitchell, R.W., Kroeger, E.A., Gilchrist, J.S.C., Moghadasian, M.M., Lukas, A. and Pierce, G.N. Effects of dietary flaxseed on vascular contractile function and atherosclerosis during prolonged hypercholesterolemia in rabbits. American Journal of Physiology Heart and Circulatory Physiology.291: H2987-2996, 2006.
[41]	Vega, G.L.,Denke, M.A. andGrundy SM. Metabolic basis of primary hypercholesterolemia. Circulation.84:118-128, 1991.
[42]	Mahfouz, M.M.and Kummerow, F.A. Cholesterol-rich diets have different effects on lipid peroxidation, cholesterol oxides, and antioxidant enzymes in rats and rabbits. The Journal of Nutritional Biochemistry. 11:293-302,2000.
[43]	Davis, P., Valacchi, G., Pagnin, E., Shao, Q., Gross, H.B., Calo, L. and Yokoyama, W. Walnuts reduce aortic ET-1 mRNA levels in hamsters fed a high fat, atherogenic diet. Journal of Nutrition.136:428-432, 2006.
[44]	Kucera, O. and Cervinkova, Z. Experimental models of non-alcoholic fatty liver disease in rats. World Journal of Gastroenterology. 20:8364-8376, 2014.
[45]	Eslamparast, T., Eghtesad, S., Poustchi, H. and Hekmatdoost, A. Recent advances in dietary supplementation, in treating non-alcoholic fatty liver disease. World Journal of Hepatology-Baishideng Publishing. 7:204-212, 2015.
[46]	Uchida, K., Takase, H., Nomura, Y., Nakamura, Y., Igimi, H., Takasu, N., Kurihara, H. and Takeuchi, N. Development of atherosclerosis in alloxan diabetic rats. Journal of Atherosclerosis and Thrombosis. 1:118-128, 1994.
[47]	Ikewuchi, C.J. and Ikewuchi, C.C. Alteration of plasma lipid profiles and atherogenic indices by Stachytarpheta jamaicensis L. (Vahl). Boikemistri. 21:71-77, 2009.
[48]	Baur, F.J. “Nutritional aspects of oils and fats,” inFood Oils and Fats: Technology, Utilization and Nutrition, Lawson, H.W. (Ed.),New York: Chapman and Hall, p. 225, 1995.
[49]	Matos, S.L., de Paula, H., Pedrosa, M.L., dos Santos, R.C., de Oliveira, E.L., Júnior, D.A. C.,Silva,M.E. Dietary models for inducing hypercholesterolemia in rats. Brazilian Archives of Biology and Technology.48:203-209, 2005.
[50]	Capanni, M., Calella, F., Biagini, M.R., Genise, S., Raimondi, L., Bedogni, G., Svegliati-Baroni, G., Sofi, F., Milani, S., Abbate, R.,Surrenti, C.andCasini, A. Prolonged n-3 polyunsaturated fatty acid supplementation ameliorates hepatic steatosis in patients with non-alcoholic fatty liver disease: a pilot study. Alimentary Pharmacology and Therapeutics.23:1143-51, 2006.
[51]	Morsi, M.K.S., Galal, S.M., El-Rahman, M.K.A. and Katry, M.A. Palm kernel oil increases the risk of coronary heart disease in rats compared with ghee. IOSR Journal of Pharmacy and Biological Sciences. 9:34-40, 2.