Proximate Composition, Phenolic Contents and in vitro Antioxidant Properties of Pimpinella stewartii (A Wild Medicinal Food)

Arshad Mehmood Abbasi^{1, 2,} and Xinbo Guo^1,

¹School of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510641, China

²Department of Environmental Sciences, COMSATS Institute of Information Technology, Abbottabad 22060, Pakistan

Cite this paper:
Arshad Mehmood Abbasi and Xinbo Guo. Proximate Composition, Phenolic Contents and in vitro Antioxidant Properties of Pimpinella stewartii (A Wild Medicinal Food). Journal of Food and Nutrition Research. 2015; 3(5):330-336. doi: 10.12691/jfnr-3-5-7

Abstract

Present study was intended to examine proximate nutritional and minerals composition, phenolic contents and in vitro antioxidant properties of Pimpinella stewartii, a commonly utilized species as medicinal and flavoring agent by the inhabitants of Pakistani Himalayas. Ethnomedicinal data were collected by semi structured interviews and questionnaires. Standard analytical methods were applied to determine nutrients, minerals and phenolic contents, where as free radical scavenging activities and total antioxidant properties were estimated by different assays. Results indicated that Pimpinella stewartii is an excellent source of carbohydrates, proteins and dietary fibers. Likewise, this specie also contains significant levels of K (6332 ± 56.1), Ca (3141 ± 47.0), Fe (1512 ± 18.7) and Mg (478.6 ±11.4) mg/kg. Comparatively, water extracts showed higher concentrations of phenolic contents than acetone extracts, showing significant difference (p<0.05). Flavonoid content was maximum in water extract at 98.67 ± 0.14 mg Rt Eq/100 g FW, followed by flavonols, total phenolics and ascorbic acid. Phosphomolibdenium complex assay (PMA) exhibited highly significant total antioxidant capacity at 86.26 ± 0.53 µM AAE/100g FW for acetone extract, followed by % DPPH radical scavenging activity (62.39 ± 0.40), whereas in water extracts measured levels were highest for ferrous ion chelating activity and ferric ion reducing antioxidant power (FRAP). Pimpinella stewartii was found rich source of nutrients and phenolic contents, and demonstrated significant in vitro antioxidant properties. Furthermore, in depth investigation of phytochemical profiling, in vivo antioxidant properties and biological activities could be useful to promote consumer health and prevention of degenerative diseases.

Keywords:
nutrients phenolics antioxidant properties Pimpinell stewartii Himalayas Pakistan

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References:

[1]	Pardo-de-Santayana, M., Tardo, J., Blanco, E., Carvalho, A.M., Lastra, J.J., San, E., Miguel and Morales, R., Traditional knowledge of wild edible plants used in the northwest of the Iberian Peninsula (Spain and Portugal): a comparative study. Journal of Ethnobiology and Ethnomedicine 3, 27. 2007.
[2]	Pieroni, A., Houlihan, L., Ansari, N., Hussain, B., Aslam, S., Medicinal perceptions of vegetables traditionally consumed by south- Asian migrants living in Bradford, Northern England. Journal of Ethnopharmacology 113,100-110. 2007.
[3]	Etkin, N., The cull of the wild. In Eating on the Wild Side: The Pharmacologic, Ecologic, and Social Implications of Using Non cultigens. Edited by: Etkin NL. Tucson/London: University of Arizona Press: 1-21. 1994.
[4]	Ercisli, S., Chemical composition of fruits in some Rose (Rosa spp.) species. Food Chemistry 104, 1379-1384. 2007.
[5]	Cruitti, P.P., Oxidant stress and carcinogenesis. European Journal of Clinical Investigation 21, 1-11. 1991.
[6]	Hazra, B., Sarkar, R., Biswas, S., and Mandal, N., Comparative study of the antioxidant and reactive oxygen species scavenging properties in the extracts of the fruits of Terminalia chebula, Terminalia belerica and Emblica officinalis. BMC Complementary and Alternative Medicines 10, 1-15. 2010.
[7]	Halliwell, B., Gutteridge, J.M.C., Free Radical Biology and Medicine (4th ed.). Oxford: Oxford University Press. 2007.
[8]	Manian, R., Anusuya, N., Siddhuraju, P., Manian, S., The antioxidant activity and free radical scavenging potential of two different solvent extracts of Camellia sinensis (L.) O. Kuntz, Ficus bengalensis L. and Ficus racemosa L. Food Chemistry 107, 1000-1007. 2008.
[9]	Krishnakumar, V., Gordon, I., Antioxidants–trends and developments. International Food Ingredients 12, 41-44. 1996.
[10]	Moure, A., Cruz, J., Franco, D., Dominguez, J., Sineiro, J., Dominguez, H., Natural antioxidants from residual sources. Food Chemistry 72, 145-171. 2001.
[11]	Zibadi, S., Farid, R., Moriguchi, S., Lu, Y., Foo, L., Tehrani, P., Oral administration of purple passion fruit peel extract attenuates blood pressure in female spontaneously hypertensive rats and humans. Nutrition Research 27, 408-416. 2007.
[12]	Ozsoy, N., Can, A., Yanardag, R., Akev, N., Antioxidant activity of Smilax excelsa L. leaf extracts. Food Chemistry 110, 571-583. 2008.
[13]	Misra, S., Maikhuri, R.K., Kala, C.P., Rao, K.S., Saxena, K.G., Wild leafy vegetables: a study of their subsistence dietetic support to the inhabitants of Nanda Devi Biosphere Reserve, India. Journal of Ethnobiology and Ethnomedicine 4, 15. 2008.
[14]	Hadjichambis, A.C.H., Hadjichambi, D.P., Della, A., Giusti, M.E., Pasquale, D.C., Lenzarini, C., Censorii, E., Tejero, M.R.G., Rojas, C.P.S., Gutierrez, J.R.R., Skoula, M., Johnson, C.H., Sarpakia, A., Hmomouchiv, M., Jorhi, S., Demerdash, M.E., Zayat, M., Pioroni, A., Wild and semi-domesticated food plant consumption in seven circum-Mediterranean areas. International Journal of Food Science and Nutrition 59(5): 383-414. 2008.
[15]	Abbasi, A.M., Shah, M.H., Li, T., Fu, X., Guo, X., Liu, RH., Ethnomedicinal values, phenolic contents and antioxidant properties of wild culinary vegetables. Journal of Ethnopharmacology 162, 333-345. 2015.
[16]	Abbasi, A.M., Shah, M.H., Khan, M.A., Wild edible vegetables of Lesser Himalayas: Ethnobotanical and Nutraceutical Aspects (Volume-1). Springer, New York. 2014.
[17]	Afzal, S., Afzal, N., Awan, A.R., Khan, T.S., Gilani, A., Rizwana, K., Sumbal, T., Ethno-botanical studies from Northern Pakistan. Journal of Ayub Medical College Abbottabad 21(1): 212-219. 2009.
[18]	Awan, M.R., Shah, M., Akbar, G., Ahmad, S., Traditional uses of economically important plants of Chitral district, Malakand division, NWFP, Pakistan. Pakistan Journal of Botany 33, 587-597. 2001.
[19]	Abuye, C., Urga, K., Knapp, H., Selmar, D., Omwega, A.M., Imungi, J.K., A compositional study of Moringa stenopetala leaves. East African Medical Journal 80, 247-252. 2003.
[20]	AOAC., Official Methods of Analysis. Association of Official Analytical Chemists, 15th Edn., Washington, DC. 1995.
[21]	Arora, M., Kiran, B., Rani, S., Rani, A., Kaur, B. & Mittal, N., Heavy metal accumulation in vegetables irrigated with water from different sources. Food Chemistry 111, 811-815. 2008.
[22]	Ji, L., Wu, L., Gao, W., Yang, J., Guo, C., Antioxidant capacity of different fraction of vegetables and correlation with the contents of ascorbic acid, phenolics, and flavonoids. Journal of Food Science 76, 1248-1257. 2011.
[23]	Lin, L., Cui, C., Wen, L., Yang, B., Luo, W., Zhao, M., Assessment of in vitro antioxidant capacity of stem and leaf extracts of Rabdosia serra (Maxim.) Hara. and identification of the major compound. Food Chemistry 126, 54-59. 2011.
[24]	Kumaran, A., Karunakaran, R.J., Antioxidant and free radical scavenging activity of an aqueous extract of Coleus aromaticus. Food Chemistry 97, 109-114. 2006.
[25]	Klein, B.P., Perry, A.K., Ascorbic acid and vitamin A activity in selected vegetables from different geographical areas of the United States. Journal of Food Science 47, 941-948. 1982.
[26]	Aoshima, H., Tsunoue, H., Koda, H., Kiso, Y., Aging of whiskey increases 1, 1–diphenyl–2–picrylhydrazyl radical scavenging activity. Journal of Agriculture and Food Chemistry 52, 5240-5244. 2004.
[27]	Yu, W., Zhao, Y., Shu, B., The radical scavenging activites of radix puerariae isoflavanoids: A chemiluminescence study. Food Chemistry 86, 525-529. 2004.
[28]	Aeyigoro, A.O., Okoh, I.A., Preliminary phytochemical screening and in vitro antioxidant activities of the aqueous extract of Helichrysum longifolium DC. BMC Complementary and Alternative Medicine 10, 1-8. 2010.
[29]	Dinis, T.C.P., Madeira, V.M.C., Almeida, M.L.M., Action of phenolic derivates (acetoaminophen, salycilate and 5-aminosalycilate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Archives of Biochemistry and Biophysics 315, 161-169. 1994.
[30]	Hazra, B., Biswas, S., Mandal, N., Antioxidant and free radical scavenging activity of Spondias pinnata. BMC Complementary and Alternative Medicine 8, 63-75. 2008.
[31]	Prieto, P., Pineda, M., Aguilar, M., Spectrophotometric Quantitation of Antioxidant Capacity through the Formation of a Phosphomolybdenum Complex: Specific Application to the Determination of Vitamin E1. Analytical Biochemistry 269, 337-341. 1999.
[32]	Abbasi, A.M., Khan, M.A., Shah, M.H., Shah, M.M., Pervez, A., Ahmad, M., Ethnobotanical appraisal and cultural values of medicinally important wild edible vegetables of Lesser Himalayas-Pakistan. Journal of Ethnobiology and Ethnomedicines 9(1): 66. 2013.
[33]	Shojaii, A., Fard, M.A., Review of Pharmacological Properties and Chemical Constituents of Pimpinella anisum, International Scholarly Research Network ISRN Pharmaceutics.
[34]	Soobrattee, M.A., Neergheen, V.S., Luximon-Ramma, A., Aruoma, O.I., Bahorun, T., Phenolics as potential antioxidant therapeutic agents: mechanism and actions. Mutation Research 579, 200-213. (2005).
[35]	Devi, R.K.S., Sreenivasulu, P., Basha, S.K.M., Antioxidant activity and total polyphenols content of certain high valued medicinal plants of Tirumala hills, Andhra Pradesh. Indian Journal of Plant Sciences 2(1):93-98. 2013.
[36]	Lu, J., Huang, G., Deng, J., Qian, W., Xu, L., Wen, C., Li, T., Wang, Z., Deng, X., Wang, D., Guan, S., Antioxidant and cytotoxic activity of the wild edible Pimpinella brachycarpa (Kom.) Nakai. American Journal of Food Technology 7(8): 494-500. 2012.
[37]	Namjooyan, F., Azemi, M.E., Rahmanian, V.R., 2010. Investigation of antioxidant activity and total phenolic content of various fractions of aerial parts of Pimpinella barbata (dc.) Boiss. Jundishapur Journal of Natural Pharmaceutical Products 5(1): 1-5.
[38]	Wong, S.P., Lai, P.L., Jen, H.W.K., Antioxidant activities of aqueous extracts of selected plants. Food Chemistry 99, 775-783. 2006.
[39]	Yasuda, T., Inaba, A., Ohmori, M., Endo, T., Kubo, S., Ohsawa, K., Urinary metabolites of gallic acid in rats and their radical scavenging effect on DPPH. Journal of Natural Products 63, 1444-1446. 2000.
[40]	Benzie, I.E.F., Strain, J.J., The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: the FRAP assay. Analytical Biochemistry 239, 70-76. 1996.
[41]	Miladi, S., Damak, M., In vitro antioxidant activities of Aloe vera leaf skin extracts. Journal de la Société Chimique de Tunisie 10, 101-109. 2008.
[42]	Gohari, A.R., Hajimehdipoor, H., Saeidnia, S., Ajani, Y., Hadjiakhoondi, A., Antioxidant activity of some medicinal species using FRAP assay. Journal of Medicinal Plants 10(37): 1-7. 2011.