Responses of Physiological Traits and Nutritional Ingredients Content in Flowers of Medicinal Chrysanthemum to Selenium Application at Different Growth Stages

Jian-Zhou Chu¹, Xiao-Qin Yao^1, , Chao Si¹, Gui-Xia Liu¹ and Chun-Hui Ma¹

¹The College of Life Sciences, Hebei University, Baoding, China

Cite this paper:
Jian-Zhou Chu, Xiao-Qin Yao, Chao Si, Gui-Xia Liu and Chun-Hui Ma. Responses of Physiological Traits and Nutritional Ingredients Content in Flowers of Medicinal Chrysanthemum to Selenium Application at Different Growth Stages. Journal of Food and Nutrition Research. 2014; 2(9):575-581. doi: 10.12691/jfnr-2-9-8

Abstract

The paper mainly studied the effects of selenium (Se) application time on physiological traits and nutritional ingredients in flowers of medicinal chrysanthemum. Flower dry weight and Se concentration in flower were significantly increased by Se application at vigorous growth, bud differentiation and bud stages. Spraying Se at seedling and flower stages evidently increased Se concentration, but did not affect flower dry weight. The rate of superoxide radical production, hydrogen peroxide (H₂O₂) and malondialdehyde concentration were significantly decreased by Se application at vigorous growth (except for H₂O₂), bud differentiation and bud stages. At five growth stages, Se application significantly increased glutathione peroxidase activity. At bud differentiation and bud stages, Se application significantly increased penylalanine ammonia lyase enzyme activity, chlorogenic acid, free amino acid and vitamin C content. These results indicated that Se applied at bud differentiation---bud stage was more beneficial for flower dry weight, Se concentration and nutritional ingredients content in flowers.

Keywords:
chrysanthemum selenium chlorogenic acid flavone free amino acid

This work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

References:

[1]	Dong, J.Z., Wang, Y., Wang, S.H., Yin, L.P., Xu, G.J., Zheng, C., Lei, C. and Zhang, M.Z., “Selenium increases chlorogenic acid, chlorophyll and carotenoids of Lycium chinense leaves,” Journal of the Science of Food and Agriculture, 93 (2), 310-315, 2013.
[2]	Cartes, P., Gianfreda, L., Paredes, C. and Mora, M.L., “Selenium uptake and its antioxidant role in ryegrass cultivars as affected by selenite seed pelletization,” Journal of Soil Science and Plant Nutrition, 11 (4), 1-14, 2011.
[3]	Schrauzer, G.N. and Meginness, J.E., “Observations on human selenium supplementation”. Trace Substances and Environmental Health, 13, 64-82, 1978.
[4]	Chilimba, A.D.C., Young, S., Black, C.R., Meacham, M.C., Lammel, j. and Broadley, M.R., “Agronomic biofortification of maize with selenium (Se) in Malawi,” Field Crops Research, 125, 118-128, 2012.
[5]	Hartikainen, H. and Xue, T., “The promotive effect of selenium on plant growth as triggered by ultraviolet radiation,” Journal of Environmental Quality, 28 (4), 1372-1375, 1999.
[6]	Hu, H.F., Hu, C.X., Jie, X.L., Liu, S.L., Guo, X., Hua, D.L., Ma, C.A., Lu, J.W. and Liu, H.G., “Effects of selenium on herbage yield, selenium nutrition and quality of alfalfa,” Journal of Agricultura and Food Chemistry, 8 (2), 792-795, 2010.
[7]	Chu, J.Z., Yao, X.Q., Yue, Z.W., Li, J.M. and Zhao, J.H., “The effects of selenium on physiological traits, grain selenium concentration and yield of winter wheat at different development stages,” Biological Trace Element Research, 151 (3), 434-440, 2013.
[8]	Feng, R.W., Wei, C.Y. and Tu, S.X., “The roles of selenium in protecting plants against abiotic stresses,” Environmental and Experimental Botany, 87, 58-68, 2013.
[9]	Tian, X.Y. and Wang, Z.Y., “Effects of selenium application on content, distribution and accumulation of selenium, flavonoids and rutin in tartary buckwheat,” Plant Nutrition and Fertilizer Science, 14 (4), 721-727, 2008 (in Chinese).
[10]	Wu, Y., Fang, M.F., Yue, M., Chai, Y.F., Wang, H. and Li, Y.F., “Advances in influence of UV-B radiation on medicinal plant secondary metabolism,” Zhongguo Zhong Yao Za Zhi, 37 (15), 2248-2251, 2012 (in Chinese).
[11]	Ke, D., Wang, A., Sun, G. and Dong, L., “The effect of active oxygen on the activity of ACC synthase induced by exogenous IAA”. Acta Botanica Sinica, 44 (5), 551-556, 2002.
[12]	Prochazkova, D., Sairam, R.K., Srivastava, G.C. and Singh, D.V., “Oxidative stress and antioxidant activity as the basis of senescence in maize leaves,” Plant Science, 161 (4), 765-771. 2001.
[13]	Li, H.S., The experimental principle and technology in plant physiology and biochemistry, Higher Education Press, Beijing, 2000, 213-214.
[14]	He, S.M. and Liu, J.L., “Study on the Determination Method of Flavone Content in Tea,” Chinese Journal of Analytical Chemistry, 35 (9), 1365-1368, 2007.
[15]	Zhang, Q.F., Liu, B., Shi, H. and Huang, S.F., “Rapid determination of chlorogenic acid in flowers of Lonicera japonica,” Journal of Fujian Medical University, 40 (4), 398-401, 2006 (in Chinese).
[16]	Hu, Q., Xu, J. and Pang, G., “Effect of selenium on the yield and quality of green tea leaves harvested in early spring,” Journal of Agricultura and Food Chemistry, 51 (11), 3379-3381, 2003.
[17]	Kong, L.G., Wang, M. and Bi, D., “Selenium modulates the activities of antioxidant enzymes, osmotic homeostasis and promotes the growth of sorrel seedlings under salt stress,” Plant Growth Regulation, 45 (2), 155-163, 2005.
[18]	Li, Y.M., Sun, Y.X. and Li, D.H., “Effects of applying selenium on contents of total flavonoid, chlorogenic acid and selenium in flower of Chrysanthemum morifolium,” Soil, 42, 618-623, 2010 (in Chinese).
[19]	Curtin, D., Hanson, R., Lindley, T.N. and Butler, R.C., “Selenium concentration in wheat (Triticum aestivum) grain as influenced by method, rate, and timing of sodium selenite application,” New Zealand Journal of Crop and Horticultural Science, 34 (4), 329-339, 2006.
[20]	Pruvot, G., Masimino, J., Peltier, G. and Rey, P., “Effects of low temperature, high salinity and exogenous ABA on the synthesis of two chloroplastic drought-induced proteins in Solanum tuberosum,” Physiologia Plantarum, 97 (1), 123-131, 1996.
[21]	Santos, I., Fidalgo, F. and Almeida, J.M., “Biochemical and ultrastructural changes in leaves of potato plants grown under supplementary UV-B radiation,” Plant Science, 167, 925-935, 2004.
[22]	Hawrylak-Nowak, B., “Beneficial effects of exogenous selenium in cucumber seedlings subjected to salt stress,” Biological Trace Element Research, 132 (1-3), 259-269, 2009.
[23]	Chu, J.Z., Yao, X.Q. and Zhang, Z.N., “Responses of wheat seedlings to exogenous selenium supply under cold stress,” Biological Trace Element Research, 136 (3), 355-363, 2010.
[24]	Ríos, J.J., Blasco, B., Cervilla, L.M., Rosales, M.A., Sanchez-Rodriguez, E., Romero, L. and Ruiz, J.M., “Production and detoxification of H2O2 in lettuce plants exposed to selenium,” Annals of Applied Biology, 154 (1), 107-116, 2009.
[25]	Foyer, C. and Noctor, G., “Oxidant and antioxidant signaling in plants: a re-evaluation of the concept of oxidative stress in a physiological context,” Plant, Cell and Environment, 28 (8), 1056-1071, 2005.
[26]	Zucker, M., “Induction of phenylalanine ammonia-lyase in xanthium leaf disks. Photosynthetic requirement and effect of daylength,” Plant Physiology, 44 (6), 912-922, 1969.
[27]	Ježek, P., Hlušek, J., Lošák, T., Jůzl, M., Elzner, P., Kráčmar, S., Buňka, F. and Martensson, A., “Effect of foliar application of selenium on the content of selected amino acids in potato tubers (Solanum tuberosum L.),” Plant, Soil and Environment, 57, 315-320, 2011.
[28]	Niki, E., “Action of ascorbic acid as a scavenger of active and stable oxygen radicals,” The American Journal of Clinical Nutrition, 54 (6 Suppl), 1119S-1124S, 1991.
[29]	Lee, G.P. and Par, K.W., “Effect of selenium concentration in the nutrient solution on the growth and internal quality of endive,” Journal of the Korean Society for Horticultural Science, 39, 391-396, 1998.
[30]	Duma, M., Alsina, I., Dubova, L., Stroksa, L. and Smiltina, Z. “The effect of sodium selenite and selenite on the quality of lettuce,” in Baltic Conference on Food Science and Technology, FoodBalt. Jelgave (Latvia), 39-44.