Effect of Drying Methods and Type of Packaging Materials on Phytochemical Content and Total Antioxidant Capacity of Five Medicinal Plants with Cosmetic Potential over Three Months Storage at Ambient Temperature

DGND Gamage¹, RM Dharmadasa^2, , DC Abeysinghe¹, RGS Wijesekara³, GA Prathapasinghe³ and Takao Someya⁴

¹Faculty of Agriculture and Plantation Management, Wayamba University of Sri Lanka, Makandura, Gonawila. 60170, Sri Lanka

²Industrial Technology Institute, 363, Bauddhaloka Mawatha, Colombo 7, Sri Lanka

³Faculty of Livestock, Fisheries and Nutrition, Wayamba University of Sri Lanka, Makandura, Gonawila. 60170, Sri Lanka

⁴ALBION Co.,Ltd, Ginza 1-7-10, Chuo-ku, Tokyo, 104-0061, Japan

Cite this paper:
DGND Gamage, RM Dharmadasa, DC Abeysinghe, RGS Wijesekara, GA Prathapasinghe and Takao Someya. Effect of Drying Methods and Type of Packaging Materials on Phytochemical Content and Total Antioxidant Capacity of Five Medicinal Plants with Cosmetic Potential over Three Months Storage at Ambient Temperature. World Journal of Agricultural Research. 2021; 9(2):73-79. doi: 10.12691/wjar-9-2-5

Abstract

Drying and storage are the most integral parts of the post-harvest practices of herbal materials. These practices directly influence the physical and chemical quality of the processed product. Therefore, the main objective of the present study was to analyze the effect of drying methods and packaging materials on total flavonoid content, total phenolic content, and total antioxidant capacity of five medicinal plant leaves with cosmetic potential. Leaves of Hibiscus rosa-sinensis L., Senna alata (L.) Roxb., Centella asiatica (L.) Urb., Ocimum tenuiflorum L. and Justicia adhatoda L. were dried to a constant weight using shade drier at 30-35°C, solar drier at 30-40°C and oven at 40°C. Thereafter, dried leaves were stored using three different packaging materials namely glass jars, polythene bags and gunny bags at ambient temperature for three months. Aluminum chloride colorimetric assay, folin-ciocalteau method, and phosphomolybdate assay were employed to analyze the total flavonoid content (TFC), total phenolic content (TPC) and total antioxidant capacity (TAC) of ethanolic extracts of leaves respectively in each month. Data were presented as mean ± standard deviation of minimum three replications. Significant interactions of the drying methods and packaging materials on TAC, TFC and TPC of dried leaf materials were analyzed using Two-way ANOVA. Results showed that the maximum TFC, TPC and TAC in oven dried H. rosa-sinensis (23.48±2.49 mg RE/100g DW, 1.09±0.24 mg GAE/100g DW and 0.39±0.05 mg AAE/100g DW respectively) and C. asiatica (128.64±10.59 mg RE/100g DW, 2.38±0.32 mg GAE/100g DW and 2.2±0.05 mg AAE/100g DW respectively) leaves stored in glass jars and solar dried S. alata (117.43±9.00 mg RE/100g DW, 3.99±0.29 mg GAE/100g DW and 1.07±0.04 mg AAE/100g DW respectively), O. tenuiflorum (216.02±0.75 mg RE/100g DW, 1.92±0.12 mg GAE/100g DW and 1.07±0.03 mg AAE/100g DW respectively) and J. adhatoda (11.13±1.23 mg RE/100g DW, 1.02±0.19 mg GAE/100g DW and 0.42±0.04 mg AAE/100g DW respectively) leaves stored in glass jars at the end of the storage period. However, statistically significant interaction (p value < 0.05) was not reported between drying method and packaging material on TPC of C. asiatica and O. tenuiflorum leaves and TFC of S. alata leaves. In conclusion, determining the effect of different processing methods on chemical constituents of aforementioned plant leaf materials is suggested to assure the quality of the final product.

Keywords:
antioxidant capacity drying methods medicinal plants packaging materials phytochemical content storage

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References:

[1]	Lisboa, C. F., Melo, E. de. C. and Donzeles, S. M. L. Influence of Storage Conditions on Quality Attributes of Medicinal Plants. Journal of scientific and technical research, 4(4), 4093-4095. 2018.
[2]	Qaas, F. and Schiele, E. Influence of energy costs on profitability in drying operations. Journal for medicinal and spice plants, 6 (3), 144-145. 2001.
[3]	Muller, J. and Heindl, A. Drying of medicinal plants. In: R. Bogers, L. Craker and D. Lange, eds. Medicinal and aromatic plants. Netherlands: Springer. 237-252. 2006.
[4]	Alamgir, A. Herbal Drugs: Their Collection, Preservation, and Preparation; Evaluation, Quality Control, and Standardization of Herbal Drugs. In: Therapeutic Use of Medicinal Plants and Their Extracts. s.l.: Springer, 453-495. 2017
[5]	Masand, S., Madan, S. and Balian, S. Modern concept of storage and packaging of raw herbs used in Ayurveda. International journl of research in Ayurveda and pharmacy, 5(2), 242-245. 2014.
[6]	Gamage, DGND., Abeysinghe, DC., Wijesekara, RGS., Prathapasinghe, GA., Dharmadasa, RM & Someya, T. Emerging Herbal Cosmetic Production in Sri Lanka: Identifying Possible Interventions for the Development of the Herbal Cosmetic Industry, Scientifica, vol. 2021, Article ID 6662404, 12 pages. 2021.
[7]	Gunathilake, K., Ranaweera, K. and Rupasinghe, H. Change of phenolics, carotenoids, and antioxidant capacity following simulated gastrointestinal digestion and dialysis of selected edible green leaves. Food Chemistry. 2017.
[8]	Gunathilake, K. and Ranaweera, K. Antioxidative properties of 34 green leafy vegetables. Journal of Functional Foods, 26, 176-186. 2016.
[9]	Gamage, DGND., Abeysinghe, DC., Wijesekara, RGS., Prathapasinghe, GA., Dharmadasa, RM & Someya, T. Assessment of Phytochemical Contents and Total Antioxidant Capacity of Five Medicinal Plants with Cosmetic Potential under Three Different Drying Methods. World Journal of Agricultural Research, 9(1): 24-28. 2021.
[10]	Mahanon, H., Azizh, A. H. and Dzulkilfly, M. H., Effect of different drying concentration of several photochemical in herbal preparation of eight medicinal plant leaves. J. Mal. Nutrition., 5, 47-54. 1999.
[11]	Bernard, D., Kwabena, A., Osei, O., Daniel, G., Elom, S. and Sandra, A. The Effect of Different Drying Methods on the Phytochemicals and Radical Scavenging Activity of Ceylon Cinnamon (Cinnamomum zeylanicum) Plant Parts. European Journal of Medicinal Plants, 4(11), 1324-1335. 2014.
[12]	Pham, H. N., Nguyen, V., Vuong, Q., Bowyer, M. and Scarlett, C. Effect of Extraction Solvents and Drying Methods on the Physicochemical and Antioxidant Properties of Helicteres hirsuta Lour. Leaves. Technologies, 3, 285-301. 2015.
[13]	Cuervo-Andrade. Quality oriented drying of Lemon Balm (Melissa officinalis L.), 2011.
[14]	Santana, P. M., Quijano-Avilés, M., Chóez-Guaranda, I., Lucas, A., Espinoza, R., Martínez, D. and Martínez, M. Effect of drying methods on physical and chemical properties of Ilex guayusa leaves. Rev. Fac. Nac. Agron. Medellín, 71(3), 8617-8622. 2018.
[15]	Chan, E., Lim, Y., Wong, S., Lim, K., Tan, S., Lianto, F. and Yong, M. Effects of different drying methods on the antioxidant properties of leaves and tea of ginger species. Food Chemistry, 113 (2009), 166-172. 2008.
[16]	Ghasemzadeh, A., Jaafar, H. Z. E. and Rahmat, A. Variation of the Phytochemical Constituents and Antioxidant Activities of Zingiber officinale var. rubrum Theilade Associated with Different Drying Methods and Polyphenol Oxidase Activity. Molecules, 21, 780. 2016.