Fermentable Sugar Production and Separation from Water Hyacinth Using Enzymatic Hydrolysis

Pradip saha^1, , Md.Fakhrul Alam¹, Ajit Chandra Baishnab¹, Maksudur Rahman Khan^{1, 2} and M. A. Islam¹

¹Department of Chemical Engineering and Polymer Science, Shahjalal University of Science and Technology, Sylhet, Bangladesh

²Faculty of Chemical and Natural Resources Engineering, University Malaysia Pahang, Pahang, Malaysia

Cite this paper:
Pradip saha, Md.Fakhrul Alam, Ajit Chandra Baishnab, Maksudur Rahman Khan and M. A. Islam. Fermentable Sugar Production and Separation from Water Hyacinth Using Enzymatic Hydrolysis. Sustainable Energy. 2014; 2(1):20-24. doi: 10.12691/rse-2-1-4

Abstract

Water hyacinth containing a remarkable amount of cellulose which is found throughout the world as unusable material that can be used as one of the promising source for the production of glucose, initial step to produce bio ethanol. In this current study different types of treatments highest glucose concentration was obtained by hot water treated method. In addition, glucose was produced from water hyacinth using cellulytic enzyme pseudomonas sp., isolated from cow dung. Glucose concentration and production rate increases with the increasing of substrate concentration and enzyme loading, particle size 45µm and a pH 6.00 and temperature 40°C are the optimum for glucose production. A kinetic model rate expression has been developed for enzymatic hydrolysis of water hyacinth based on the Michaelis – Mentens model and parameters are determined. 0.15gm Reducing sugar are separated from the mixture of glucose water solution using 2 gm of water hyacinth. 0.531mg/l Cellulytic composition in water hyacinth are determined.

Keywords:
hydrolysis water hyacinth pseudomonas sp.Michaelis – Mentens kinetic model

This work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

References:

[1]	Bentley, R W, Global oil and gas depletion: an overview. Energy Policy.30: 189-205, 2002.
[2]	Cavallo, A J, “Predicting the peak in world oil production,” Nat Resour.Res. 11: 187-195, 31: 426-428,2002.
[3]	Kadam, K L, Rydholm, EC, and McMillan JD, “Development and Validation of a Kinetic Model for Enzymatic Saccharification of Lignocellulosic Biomass,” Biotechnol.Prog, 20, 698-705, 2004.
[4]	Y. Zhang, Jing, LX, Hui, JX, Zhen, H.Y, and Ying G. “Cellulase deactivation based kinetic modeling of enzymatic hydrolysis of steam-exploded wheat straw,” Bioresource Technology, 101 8261-8266, 2010.
[5]	Zhang, Y., Zhang, D., and Barrett S. “Genetic uniformity characterises the invasive spread of water hyacinth (Eichhorniacrassipes), a clonal aquatic plant,” Molecular Ecology, 19: 1774-1786, 2010.
[6]	Téllez, T., López E., Granado G., Pérez E., López R., and Guzmán J, “The water hyacinth, Eichhorniacrassipes: an invasive plant in the Guadiana River Basin (Spain),” Aquatic Invasions 3, 42-53. 2008.
[7]	Shanab, S,, Shalaby, E., Lightfoot, D. and El-Shemy, H., “Allelopathic effects of water hyacinth (Eichhorniacrassipes),” PLoS One 5(10):e13200, 2010.
[8]	Rubin, EM. “Genomics of cellulosic biofuels,” Nature 454, 841-845,2008.
[9]	Balat M, “Production of bioethanol from lignocellulosic materials via the biochemical pathway: A review,” Energy conversion and Management 52 858-875, 2011.
[10]	Hagerdal, HB, Galbe M, Gorwa-Grauslund MF, Liden G, and Zacchi G, “Bioethanol – the fuel of tomorrow from the residues of today,” Trends Biotechnol; 24:549-56, 2006.