Effect of Osmotic Stress and Nutrient Starvation on the Growth, Carotenoid and Lipid Accumulation in Dunaliella salina A9

Trung Vo^1, , Truc Mai², Hong Vu¹, Dan Van¹, Hien Dao¹, Phong Tran¹, Ngoc Nguyen¹, Phuc Nguyen¹ and Nguyen C. Nguyen³

¹Department of Biochemistry and Toxicology, Nguyen Tat Thanh University, Viet Nam

²Department of Plant and Environmental Sciences, New Mexico State University, New Mexico, USA

³Department of Biotechnology, International University-VNU, Viet Nam

Cite this paper:
Trung Vo, Truc Mai, Hong Vu, Dan Van, Hien Dao, Phong Tran, Ngoc Nguyen, Phuc Nguyen and Nguyen C. Nguyen. Effect of Osmotic Stress and Nutrient Starvation on the Growth, Carotenoid and Lipid Accumulation in Dunaliella salina A9. Research in Plant Sciences. 2017; 5(1):1-8. doi: 10.12691/plant-5-1-1

Abstract

Dunaliella salina A9 is unicellular green alga isolated from the saltern, Khanh Hoa province, Viet Nam. The effect of halostress and nutrient starvation was studied in this alga to estimate the growth, chlorophyll content and capacity of carotenoid and lipid accumulation. The results showed decrease in cell number and chlorophyll content as Dunaliella salina in response to a change from the optimal medium 1.5M NaCl to hypo-osmotic medium (0.5M NaCl) and hyper-osmotic medium (3.5M NaCl). We also observed decrease in cell count in nutrient starvation after 9 days of culture in MD4 medium. Salinity stress has more severe effect on the growth of Dunaliella salina A9 with greater decrease in cell number compared to nutrient starvation. The stress induced increasing carotenoid and lipid accumulation in cells. However the carotenoid and lipid accumulation in hypo-osmotic stress and the nutrient starvation were higher than in hyper-osmotic stress. The results suggested negative relationship between the growth rate, chlorophyll content and carotenoid, and lipid accumulation of Dunaliella salina under stress conditions.

Keywords:
Dunaliella Dunaliella salina A9 carotenoid lipid sulfo-phospho-vanillin reagent

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