Preparation, Microanalysis and Performance of Hap/Cs-Cmc Composite Materials

Mande Qiu^{1, 2,} , Aimei Dai¹, Pan Yang¹, Miao Niu¹, Yidan Wang¹ and Guoyi Bai¹

¹College of Chemistry and Environmental Science，Hebei University，Baoding 071002，People’s Republic of China;

²Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, Hebei University No.180 WUSI EAST Road Baoding China

Cite this paper:
Mande Qiu, Aimei Dai, Pan Yang, Miao Niu, Yidan Wang and Guoyi Bai. Preparation, Microanalysis and Performance of Hap/Cs-Cmc Composite Materials. American Journal of Materials Engineering and Technology. 2015; 3(2):46-50. doi: 10.12691/materials-3-2-4

Abstract

The physical and chemical properties of the materials are generally determined by their microstructure and main components. In this paper, the Hap/Cs-Cmc composite materials with different mass ratios were prepared for the first time through liquid co-precipitation method. The microstructure, phase and performance of the composite materials were investigated by FT-IR, XRD, TG-DTA, SEM, and EDS respectively. The purpose of this work is to establish the relationship between material microstructures and properties. The results showed that the composite materials exhibited excellent mechanical performance and thermal stability. The nano-Hap with relatively good crystallinity were dispersed uniformly in the organic phase Cs and Cmc-combined with relatively closely between Hap particles and Cs-Cmc. The particle size of the material is about 50 nm with spherical shape. The mass ratio of Hap and Cs-Cmc directly influenced the crystallization, particle size, and dispersion of Hap. When mass ratio is 50/50, the uniformity, compactness, and thermal stability were the best and the compressive strength is up to 30.5 MPa. EDS analysis showed that the composite material merely contained trace amount of sodium and the ratio of calcium and phosphorus is around 1.85, belonging to the rich calcium type of Hap, and the physical and chemical performance totally met the requirements of bone tissue engineering materials.

Keywords:
nano-hydroxyapatite chitosan carboxymethyl cellulose composite materials microanalysis

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References:

[1]	Kamitakahara M,Ohtsuki C,Miyazaki T. Review paper:behavior of ceramic biomaterials derived from tricalcium phosphate in physiological condition .J Biomater Appl, 2008, 23 (3) :197-212 .
[2]	Patrícia C. Salgado1,Plínio C. Sathler,et al. Bone Remodeling, Biomaterials and Technological Applications: Revisiting Basic Concepts ,Journal of Biomaterials and Nanobiotechnology[J], 2011,2,318-328.
[3]	Xiaofeng Pang, Hongjuan Zeng, Jialie Liu. The Properties of Nanohydroxyapatite Materials and its Biological Effects[J]. Materials Sciences and Applications, 2010, 1, 81-90 .
[4]	D.Alves Cardoso,J.A.ansen,S.C.G.Leeuwenburgh.Synthesis and application of nanostructured calcium phosphate ceramics for bone regeneration[J]. J Biomed Mater Res Part B 2012, 100B: 2316-2326.
[5]	Mututuvari TM, Harkins AL, Tran CD. Facile synthesis, characterization, and antimicrobial activity of cellulose-chitosan-hydroxyapatite composite material: A potential material for bone tissue engineering. J Biomed Mater Res Part A 2013: : 3266-3277.
[6]	Lee S-B, Kwon J-S, Lee Y-K, Kim K-M, Kim K-N. Bioactivity and mechanical properties of collagen composite membranes reinforced by chitosan and b-tricalcium phosphate. J Biomed Mater Res Part B 2012: 100B: 1935-1942.
[7]	Shao Zhi Fu, Xiu Hong Wang, Gang Guo, et al.Preparation and properties of nano-hydroxyapatite/PCL-PEG-PCL composite membranes for tissue engineering applications.Journal of Biomedical Materials Research B: Applied Biomaterials, 2011, 97B, 1:74-83.
[8]	Ferdous Khan, Sheikh Rafi Ahmad. Polysaccharides and their derivatives for versatile tissue engineering application . Macromol. Biosci. 2013, 13, 395-421.
[9]	Hong Li,, Chang-Ren Zhou, Min-Ying Zhu,et al. Preparation and Characterization of Homogeneous Hydroxyapatite/Chitosan Composite Scaffolds via In-Situ Hydration[J]. Journal of Biomaterials and Nanobiotechnology, 2010, 1, 42-49.
[10]	Cheng X, Li Y, Zuo Y, Zhang L, Li J, Wang H. Properties and in vitro biological evaluation of nano-hydroxyapatite/chitosan membranes for bone guided regeneration. Mater Sci Eng C 2009; 29: 29-35.
[11]	Rabea EI, Badawy MET, Stevens CV, Smagghe G, Steurbaut W.Chitosan as antimicrobial agent: Applications and mode of action.Biomacromolecules 2003; 4: 1457-1465.
[12]	Chen JP, Chen SH, Lai GJ. Preparation and characterization of biomimetic silk fibroin/chitosan composite nanofibers by electrospinningfor osteoblasts culture. Nanoscale Res Lett 2012; 8: 170-180.
[13]	Chen J, Nan K, Yin S, Wang Y, Wu T, Zhang Q. Characterization and biocompatibility of nanohybrid scaffold prepared via in situ crystallization of hydroxyapatite in chitosan matrix. Colloids Surf B, 2010; 81:640-647.
[14]	Tanase CE, Popa MI, Verestiuc L. Biomimetic chitosan–calcium phosphate composites with potential applications as bone substitutes: Preparation and characterization. J Biomed Mater Res Part B 2012: 100B: 700-708.
[15]	Alves Cardoso D, Jansen JA, G. Leeuwenburgh SC. Synthesis and application of nanostructured calcium phosphate ceramics for bone regeneration. J Biomed Mater Res Part B 2012: 100B: 2316-2326. material for bone tissue engineering. J Biomed Mater Res Part A 2013: : 3266-3277.
[16]	Nicole Y. C, et al. Review biodegradable poly(a-hydroxy acid) polymer scaffolds for bone tissue engineering .Polymers For Bone Tissue Engineering. 2010, 285-295.
[17]	Bala′ zsi C, Bishop A, Yang JHC, Bala′ zsi K, We′ ber F, Gouma PI.Biopolymer-hydroxyapatite scaffolds for advanced prosthetics.Compos Interfaces 2009;16:191-200.
[18]	Rajeswari Ravichandran, et al. Advances in polymeric systems for tissue engineering and biomedical applications. Macromol. Biosci. 2012, 12, 286-311.
[19]	Phisalaphong M, Jatupaiboon N, Kingkaew J.Biosynthesis of cellulose-chitosan composite. In: Kim S, editor. Chitin, Chitosan, Oligosaccharides and their Derivatives: Biological Activities andApplications. New York: CRC Press; 2011, 53-65.