Isolation of Biosurfactant Producing Bacteria from Garbage Soil

M. Rajesh¹, M. Samundeeswari¹ and B. Archana^1,

¹Department of Zoology, The American College, Madurai, Tamil Nadu, India

Cite this paper:
M. Rajesh, M. Samundeeswari and B. Archana. Isolation of Biosurfactant Producing Bacteria from Garbage Soil. Journal of Applied & Environmental Microbiology. 2017; 5(2):74-78. doi: 10.12691/jaem-5-2-3

Abstract

Biosurfactants are amphiphilic compounds secreted extra-cellularly by microorganisms. Biosurfactants contains hydrophobic and hydrophilic moieties that reduce the surface tension and interfacial tension by accumulating at the interface of hydrophobic substances and thus increase the solubility, bioavailability and subsequent biodegradation of the hydrophobic or insoluble organic compounds. The present study focused on the isolation of biosurfactant producing microorganisms from garbage soil. Isolated microorganisms were identified based on the biochemical characterization as Staphylococcus aureus and Bacillus cereus. Qualitative analysis of biosurfactant production was identified by performing assays like hemolysis on blood agar, penetration assay and C-TAB agar assay. The present study confirms that isolated microorganisms have biosurfactant property.

Keywords:
biosurfactant staphylococcus aureus bacillus cereus

This work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

Figures

References:

[1]	Al-Bager. S.D (2005). Bacillus cereus – King Saud University, College of Pharmacy, Department of Pharmaceutics, Section of Microbiology.
[2]	Anandara.B and P. Thivakaran (2010). Isolation and production of Biosurfactant producing organism from oil spilled soil, Journal of Biological science and Technology volume 1, ISSN: 120-126.
[3]	Chandran . P abd Nilanjana das (2010). Biosurfactant and diesel oil degradation by yeast species, Trichosporon asahii isolated from petroleoum hydrocarbon contaminated soil.International Journal of Engineering Science and Technology Vol. 2(12), 6942-6953.
[4]	Kiran G.S, Hema. T.A, Gandhimathi.R, Joseph selvin, Anto Thomas. T, Rajeetharavji. T and Natarajaseenivasana .K (2009), Optimization and production of a biosurfactant from sponge associated marine fungus Aspergillusustus MSF3. Colloids surface. B., 73(2), 250-256.
[5]	Li Zhu.L, Feng-cai Zou, Yu-lin Yan, Qi-hui Wang, Yong-qiang Shi, and Wei-jie Qu (2016), The Characteristics of Staphylococcus aureus Small Colony Variant Isolated from Chronic Mastitis at a Dairy Farm in Yunnan Province, China. The Scientific World Journal Volume 2016, Article ID 9157605, 8 pages.
[6]	Muller-Hunting. R, Wagner. F, Blaszczyk. R, Kosaric. N (1993), Biosurfactants production, properties, application. Kosaric N ed. 447-469.
[7]	Rita de Cássia F. S. Silva, Darne G. Almeida, Raquel D. Rufino, Juliana M. Luna, Valdemir A. Santos and Leonie Asfora Sarubbo (2014), Applications of Biosurfactants in petroleum industry and the remediation of oil spills. International journal of molecular science- ISSN 1422-0067.
[8]	Sim.L, Ward. O.P, Lizy (1997). Production and characterization of biosurfactant isolated from Pseudomonas aeroginousa UW-1. Journal of Industrial Microbiology and Biotechnology.19, 232-236.
[9]	Sneha-Chakrabarti (2013). Bacterial Biosurfactant: Characterization, Antimicrobial and metal remediation properties.
[10]	Varjani. J.S, D. P. Rana, S. Bateja, M. C. Sharma and V. N. Upasani (2014). Screening and identification of biosurfactant (bioemulsifier) producing bacteria from crude oil contaminated sites of Gujarat, India. International Journal of Innovative Research in Science, Engineering and Technology – Vol. 3, Issue 2.
[11]	Vijaya. B, N.R. Jayalakshmi and K. Manjunath (2013). Enumeration of biosurfactant producing microorganisms from oil contaminated soil in and around Bangalore (India). International journal of current science, 5: 86-94.
[12]	Walter, Christoph Syldatk and Rudolf Hausmann, (2010). Screening Concepts for the Isolation of Biosurfactant Producing Microorganisms.