Bioethanol Production from Anacardium occidentale Fruit Juiceand Preparation of Blended Fuel Using In-house Developed Bioreactor

Mohanasrinivasan V.^1, , Anand E.¹, SubathraDevi C.¹, Jemimah Naine S.¹ and Karthikeyan S.¹

¹School of Biosciences and Technology, VIT University, Vellore, Tamil Nadu, India

Cite this paper:
Mohanasrinivasan V., Anand E., SubathraDevi C., Jemimah Naine S. and Karthikeyan S.. Bioethanol Production from Anacardium occidentale Fruit Juiceand Preparation of Blended Fuel Using In-house Developed Bioreactor. Journal of Applied & Environmental Microbiology. 2015; 3(2):44-48. doi: 10.12691/jaem-3-2-3

Abstract

Ethanol has been known for a long time, being perhaps the oldest product obtained through traditional biotechnology. In the present research work, bioreactor was designed to produce bioethanol from Anacardium occidentale using Zymomonas mobilis MTCC 2427. A minimum requirement bioreactor was designed for the mass production of ethanol. Anacardium occidentale substrate was pretreated, total and reducing sugar concentrations were determined and found to be 3.4 mg/mL and 1.8 mg/mL, respectively. During fermentation the bacterial load, sugar content and ethanol content were analyzed for five days, where the highest concentration of ethanol (28%) was obtained on the third day of fermentation and the corresponding bacterial load was 15x10³CFU. Ethanol obtained from the fermentation studies were further analyzed by FT-IR. The obtained ethanol was blended with petrol in different concentrations E5%, E10%, E15% and E20% respectively and successfully tested in four stroke engine.

Keywords:
Zymomonas mobilis MTCC 2427 bioethanol bioreactor fermentation Anacardium occidentale cashew apple

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