Isolation and Screening of Dye Decolorizing Bacteria

M P Shah^1,

¹Industrial Waste Water Research Laboratory Division of Applied & Environmental Microbiology Lab Enviro Technology Limited Gujarat, India

Cite this paper:
M P Shah. Isolation and Screening of Dye Decolorizing Bacteria. Journal of Applied & Environmental Microbiology. 2014; 2(5):244-248. doi: 10.12691/jaem-2-5-7

Abstract

The present study reveals that the enriched aerobic cultures of indigenous microbes can be used successfully for decolorizing dye effluents. Physico-chemical analysis of dye effluent revealed high load of pollution. Textile dye effluent and contaminated soils were collected and analyzed for selection of suitable bacteria for dye degradation. The residual bacterial load was found to be in the range of 10⁸ cfu.mL^-1. Six bacterial species viz., two species of both Bacillus and Klebsiella,, and one species of Planococcus and Micrococcus were isolated. The best two species of dye degraders namely Planococcus and Bacillus were further optimized for the effect of carbon and nitrogen source, pH, temperature and percentage of inoculums. The optimized conditions for both isolates of Planococcus sp. and Bacillus sp. were used in bio-decolorization studies of textile effluent. More than 50% of decolorization was achieved within 4 days of incubation while 80% of decolorization after 6 days. The isolates of Planococcus sp. and Bacillus sp. exhibited maximum decolorization ability at pH between 5-8 and temperature 37C. Moreover, 10% (v/v) inoculums, glucose and peptone as carbon and nitrogen sources respectively were found to be the optimum conditions for decolorization. Both those isolates showed highest decolorization percentage of Coractive Blue 3R dye effectively during optimization.

Keywords:
Textile effluent Bacillus Klebsiella Planococcus Micrococcus decolorization

This work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

References:

[1]	Akhilesh, D., Neeraj, M, Neha, S, Abhina, S. and Shivendra, V. 2010. Isolation of dye degrading microorganism. Elec. J. Environ. Agri. Food Chem. 9 (9): 1534-1539.
[2]	Aksu, Z. 2005. Application of biosorption for the removal of organic pollutants: A review. Proc. Biochem. 40: 997-1026.
[3]	Aksu, Z., Kilic, N, Ertugrul, V. and Donmez, G. 2007. Inhibitory effects of chromium (Vl) and Remazol black on chromium (Vl) and dye stuff removals by Trametes versicolor. Enz. Microbial Technol. 40: 1167-1174.
[4]	APHA. 1992. Standard methods for examination of water and waste water. APHA, AWWA. Washington, DC., USA.
[5]	Axelsson, J., Nilsson, U., Terrazas, E., Aliaga, T.A. and Welander, U. 2006. Decolorization of the textile dyes reactive red 2 and reactive blue for using Bjerekandera sp. strain Bol 13 in a continuous rotating biological contactor reactor. Enz. Microbial Technol. 39: 32-37.
[6]	Banat, I.M., Nigam, P., Singh, D. and Marchant, R. 1996. Microbial decolorization of textile dye containing effluents: A review. Biores. Technol. 58: 217-227.
[7]	Buckley, C.A. 1992. Membrane technology for the treatment of dye house effluents. Water Sci. Technol. 25 (10): 203-209.
[8]	Gopi, V., Akhilesh, U. and Soundararajan, N. 2012. Bioremediation potential of individual and consortium non-adapted fungal strains on Azo dye containing textile effluent. Adv. Appl. Sci. Res. 3 (1): 303-311.
[9]	Hao, O.J, Kim, H. and Chaing, P.C. 2000. Decolorization of wastewater critical reviews. Environ. Sci. Technol. 30: 449-505.
[10]	Holt, J.G., Krig, N.R., Sneath, P.H.A., Staley, J.T. and Williams, S.T. 1994. Bergey's manual of determinative bacteriology (9th edn). Baltimore, Maryland: Williams and Wilkins.
[11]	Jayarajan, M., Arunachalam, R. and Annadurai, G. 2011. Agricultural wastes of jackfruit peel nano-pourous adsorbent for removal of rhodamine dye. Asian J. Appl. Sci. 4: 263-270.
[12]	Kumar, K., Dastidar, M.G. and Sreekrishnan, T.R. 2009. Effect of process parameters on aerobic decolorization of reactive azo dye using mixed culture. World Acad. Sci. Engg. Technol. 58: 962-965.
[13]	Martin, D., Stanley, F., Eugene, R., Karl-Heinz, S. and Erok, S. 2006. The prokaryotes: A hand book on the biology of bacteria, 3rd edn. Vol-I-VII.
[14]	Moorthi, P.S., Selvam, S., Sasikalaveni, A, Murugesan, K. and Kalaichelvan, P.T. 2007. Decolorization of textile dyes and their effluents using white rot fungi. African J. Biotech. 6 (4): 424-429.
[15]	Nigam, P., Geoff, M., Banat, I.M. and Roger, M. 1996. Decolorization of effluent from the textile industry by a microbial consortium. Biotechnol. Lett. 18 (1): 117-120.
[16]	Ponraj, M., Gokila, K. and Vassudeo Zambare. 2011. Bacterial decolorization of textile dye-orange 3R. Int. J. Adv. Biotechnol. Res. 2: 168-177.
[17]	Prasad, A.A.S. and Rao, K.V.B. 2010. Physicochemical characterization of textile effluent and screening for dye decolorizing bacteria. Global J. Biotechnol. Biochem. 5 (2): 80-86.
[18]	Saranraj, P., Sumathi, V., Reetha, D. and Stella, D. 2010. Decolorization and degradation of direct azo dyes and biodegradation of textile dye effluent by using bacteria isolated from textile dye effluent. J. Ecobiotechnol. 2 (7): 7-11.
[19]	Tyagi, O.D. and Mehra, M. 1990. A textbook of environmental chemistry. Anmol Publications, New Delhi, India.
[20]	Wang, H., Su, J.Q., Zheng, X.W., Tian, Y., Xiong, X.J. and Zheng, T.L. 2009. Bacterial decolorization and degradation of the reactive dye Reactive Red 180 by Citrobacter sp. CK3q. Int. Biodeterioration Biodegradation. 63: 395-399.