Isolation of Fluorescent Pseudomonas spp. from Degraded Agricultural Soils in the Daloa Region: Salt Stress Resistance Capacity

Toualy Serge Thibaut OUINA^1, , Rosin Don Rodrigue VOKO Bi¹, Kouamé Claude YA¹, Pascaline Emira Wilfried KOUAMɹ, Marina KOUSSEMON-CAMARA² and Ibrahim KONATE¹

¹Laboratory of Agrovalorisation, Department of Biochemistry and Microbiology, University Jean LOROUGNON GUEDE, Daloa, Côte d’Ivoire

²Laboratory of Biotechnology and Food Microbiology, Department of Food Sciences and Technology, University Nangui Abrogoua, Abidjan, Côte d’Ivoire

Cite this paper:
Toualy Serge Thibaut OUINA, Rosin Don Rodrigue VOKO Bi, Kouamé Claude YA, Pascaline Emira Wilfried KOUAMÉ, Marina KOUSSEMON-CAMARA and Ibrahim KONATE. Isolation of Fluorescent Pseudomonas spp. from Degraded Agricultural Soils in the Daloa Region: Salt Stress Resistance Capacity. Journal of Applied & Environmental Microbiology. 2025; 13(1):33-40. doi: 10.12691/jaem-13-1-5

Abstract

Salinisation due to intensive agriculture and geological alteration reduces soil microbial activity, creating unfavorable conditions for plant growth. This study aimed at screening degraded banana plantation soils from Digbapia and Zokoguhé (Daloa, Côte d’Ivoire) for fluorescent Pseudomonas spp. able to tolerate saline stress. Isolates were recovered, assayed for extracellular enzymatic activities, and tested for growth across a NaCl and KNO₃ gradient up to 10%. Soil samples were collected from six degraded banana plantation sites. Fluorescent Pseudomonas spp. were isolated using culture techniques on media King’s A and B. Isolates were subsequently assayed for amylase, cellulase, protease and lipase activities and evaluated by monitoring growth in media supplemented with NaCl and KNO₃ at concentrations ranging from 0 to 10%. Eight Pseudomonas species coded C226, C211, C127, C213, C212, C112R, C2211 and C129 were isolated from these soils. Among these isolates, C112R and C226 showed amylolytic activity, while C212, C213 and C127 showed cellulolytic activity. For high concentrations (8 to 10%), in KNO₃ between 791.27 and 989 mM then in NaCl between 1368.9 and 1711.1 mM, the isolates able to grow were C129, C213, C127, C2211 and C112R. These species are the best candidates for resistance to salt stress. They could serve as plant biostimulants under saline conditions, contributing to soil restoration.

Keywords:
salinity Pseudomonas enzymes degraded soil agriculture

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References:

[1]	Xing, Y., Xie, Y. and Wang, X., "Enhancing soil health through balanced fertilization: a pathway to sustainable agriculture and food security", Frontiers in Microbiology, 16, 1536524, 2025.
[2]	Wei, X., Xie, B., Wan, C., Song R., Zhong W., Xin, S. and Song, K., "Enhancing soil health and plant growth through microbial fertilizers: mechanisms, benefits, and sustainable agricultural practices", Agronomy, 14, 609, 2024.
[3]	Morales, J., "Microbial allies: enhancing soil fertility and sustainable agriculture", Scientific Journal, 2(2), 1-4, 2024.
[4]	Anstat, Population totale, valeur actuelle, National Statistics Agency, 2025. [online] Available:https://www.anstat.ci/indicateur-details/ c07c2041928f267e325dae 403d34a6e 7b033e3be80b7 afe6edcc 14544b8d1f 827330a 65f7be9f89 b66413c2e6a 34f8d9122bc 483bf274fb de8b97a28 2e9fd702asPARU vryiahv8N5 ud5dF HdccsfiLSRVrBIl01eK8gA. [Accessed September 14, 2025].
[5]	WHO, Côte d'Ivoire, health data overview for the Republic of Côte d'Ivoire, World Health Organization, 2025. [online] Available: https://data.who.int/countries/384 [Accessed July 14, 2025]
[6]	Traoré, S., Kobenan, K., Kouassi, K.S. and Gnonhouri G., "Systèmes de culture du bananier plantain et méthodes de lutte contre les parasites et ravageurs en milieu paysan en Côte d’Ivoire", Journal of Applied Biosciences, 19, 1094–1101, 2009.
[7]	Omar, A.A., Edinam, K.G and Olavi, L., "Cause and impacts of land degradation and desertification: case study of the sudan", International Journal of Agriculture and Forestry, 3(2), 40- 45, 2013.
[8]	Marlet, S. and Job, J.O., Processus et gestion de la salinité des sols. In: Tiercelin, J.R., Traité d’irrigation, seconde édition", Tec & Doc Lavoisier, Paris (France), 2006, 1-28.
[9]	Boualla, N., Benziane, A. and Derrich, Z., "Origine de la salinisation des sols de la plaine de M’Leta (bordure sud du bassin Sebkha Oran)", Journal of Applied Bioscences, 53, 3787- 3796, 2012.
[10]	Lugtenberg, B.J.J., Malfanova, N., Kamilova, F. and Berg, G., "Plant growth promotion by microbes. In: Molecular Microbial Ecology of the Rhizosphere", Frans J. de Bruijn, Pays-Bas, 2013, 561- 573.
[11]	Anonymous 1 (Department of Agriculture), La banane dessert et plantain Genre Musa. Fiche technique de la directive dans l’agriculture Polynésie Française, Department of Agriculture, Lille (France), 2018, 1-12.
[12]	Adjiri, A.O., Bintou, K., Natchia, A., Ibrahim, D. and Brou, D., "Caractérisation physico-chimique et source de la minéralisation des eaux souterraines des départements Daloa et Zoukougbeu (Côte d’Ivoire)", Journal of Applied Biosciences, 13(4), 2388-2401, 2019.
[13]	Sangaré S.K., Bilgo A., Sanson A., Dabiré P., Hien V. & Duponnois R., Fertilité des sols et gestion du potentiel infectieux mycorhiziene. In: champignons symbiotiques contre la desertification (écosytèmes mediterranéens, tropicaux et insulaires), Edition IRD, Marseille (France), 2009, 95-123.
[14]	Zro, F.G.B., Guéi, A.M., Nangah, Y.K., Soro, D. and Bakayoko S. "Statistical approach to the analysis of the variability and fertility of vegetable soils of Daloa (Côte d’Ivoire)". African Journal of Soil Science, 4 (4), 328-338, 2016.
[15]	Anonymous 2, Map of the department of Daloa, Google my map, 2025. [online] Available: https:// www.google.com /maps/@ 6.9511535,-6.699364, 11z/data=!4m5! 1m2!10m1!1e1! 11m1!3e1? hl=fr&entry=ttu&g_ep=EgoyMDI1MTAxNC4wIKXMDSoASAFQAw%3D%3D [Accessed October 2, 2025].
[16]	Yusran, Y., "Isolation and screening of Pseudomonas fluorescens isolates against Fusarium oxysporum f. sp. radicis-lycopersici and their effects on seedling growth of Paraserianthes falcataria", Biodiversitas, 24(4), 2294-2301, 2023.
[17]	Ouina, T.S., Jean, M.P., Stephane, G., David, G., Jean, P.R., Tia, J.G. and Koussémon M., "Cropping practices and fungal contamination in banana plantation in Cote d’Ivoire", Research Journal of Microbiology, 15(3), 98-108, 2017.
[18]	Mishra, A.K., Yadav, P., Sharma, S. and Maurya, P., "Comparison of microbial diversity and community structure in soils managed with organic and chemical fertilization strategies using amplicon sequencing of 16 s and ITS regions", Frontiers in Microbiology, 15, 1444903, 2025.
[19]	Kopittkea, P.M., Menziesa, N.W., Wangb, P., McKennaa, B.A. and Lombi, E., "Soil and the intensification of agriculture for global food security", Environment International, 132, 105078, 2019.
[20]	Anuroopa, N., Ram, A.B., Ranadev P, Ashwin, R., Bagyaraj, D.J., "Pseudomonas species in soil as a natural resource for plant growth promotion and biocontrol characteristics - An overview", Madras Agricultural Journal, 108, 1-13, 2021.
[21]	Aagot, N., Nybroe, O. Nielsen P. and Johnsen K., "An altered Pseudomonas diversity is recovered from soil by using nutrient-poor Pseudomonas-selective soil extract media", Applied And Environmental Microbiology, 67(11), 5233–5239, 2001.
[22]	Mukherjee, K., Prosun Tribedi, P., Chowdhury, A., Ray, T., Joardar, A., Giri, A., Sil, A.K., "Isolation of a Pseudomonas aeruginosa strain from soil that can degrade polyurethane diol", Biodegradation.
[23]	Walsh, U.F., Morrissey, J.P., and O'Gara, F., "Pseudomonas for biocontrol of phytopathogens: from functional genomics to commercial exploitation", Current Opinion in Biotechnology, 12(3), 289-295, 2001.
[24]	Tang, A., Caballo, R.A., Marquart, M., Bierdeman, A.M. and O’Callaghan, R., "Mechanism of Pseudomonas aeruginosa small protease (PASP), a corneal virulence factor", Investigative Opthalmology & Visual Science, 59 (15), 5993-6002, 2018.
[25]	Andrade, L.A., Santos, C.H.B., Frezarin, E.T., Sales L.R. and Rigobelo, E.C., "Plant growth -promoting rhizobacteria for sustainable agricaltural production", Microprganisms, 11(4), 10-88, 2023.
[26]	Choudhary, R., "Isolation and screening of Lipase producing bacteria from oil millefluent", Indian Journal of science and research, 13(2), 192-194, 2017.
[27]	Galdino, A.C.M., Branquinha, M. and Souza dos Santos, A.L., Pseudomonas aeruginosa and its arsenal of proteas: weapons to battle the host. In: Pathophysiological aspects of proteases, Chakraborti S. and Dhalla N.S (Eds), Rio de Janeiro (Brazil), 2017, 381-397.
[28]	Voisin, A.S., Cellier, P. and Jeuffroy, M.H., "Fonctionnement de symbiose fixatrice de N2 des legumineuses à graines: Impacts agronomiques et environnementaux". Innovation agronomique, 43, 139-160, 2015.
[29]	Klinfoong, R., Thummakasorn, C., Ungwiwatkul, S., Boontanom, P. and Chantarasiri, A., Diversity and activity of amylase-producing bacteria isolated from mangrove soil in Thailand, Biodiversitas, 23(10), 5519-5531, 2022.
[30]	Bhat, M.K., "Cellulases and Related Enzymes in Biotechnology", Journal of Biotechnology Advances, 18, 355-383, 2000.
[31]	Lemanceau, P.P., Mazurier, S., Pivato, B. and Avoscan, L., Compréhension et valorisation des interactions entre plantes et microorganismes telluriques: des enjeux majeurs en agroécologie. Les sols et la vie souterraine: des enjeux majeurs en agroécologie, QUAE, Paris (France), 2017, 328 p. 978-2-7592-2651-1. ffhal-01607980.
[32]	Goel, N. and Verma, K.S., "Purification and characterization of cellulase from Pseudomonas sp. Isolated from waste dumping site soil", Journal of Applied Biotechnology & Bioengineering, 6(3), 118-124, 2019.
[33]	Adjanohoun, A., Baba-Moussa, L.S., Dagbénonbakin, G., Saïdou, A. and Toukourou, F., Utilisation des microorganismes du sol pour accroître la productivité agricole: Manuel de l’apprenant. CNSMaïs/INRAB/SNRA. Calavi (Bénin), 2017, 76 p.
[34]	Diaw, D., Fall-Ndiaye, M.A., Oubeidillah Youssoufa Ali, O.Y., Sare I.C. and DIOP T.A., " Effect of salinity on the density of fluorescent Pseudomonas spp isolates from the rhizosphere of tomato, eggplant and onion plants in Senegal ", International Journal of Biological and Chemical Sciences, 12(4), 1914-1919, 2018.
[35]	Kim, S., Ha, J., Lee, H., Lee, S., Lee, J, Choi, Y., Oh, H., "Role of Pseudomonas aeruginosa DesB in Adaptation to Osmotic Stress", Journal of Food Protection, 82(8), 1278-1282, 2019.
[36]	Costa-Gutierrez, S.B., Caram-Di, S.M.C.d.V., Zenoff, A.M., Espinosa-Urgel, M., de Cristóbal, R.E. and Vincent, P.A., "Isolation of Pseudomonas strains with potential for protection of soybean plants against saline stress", Agronomy, 11, 2236, 2021.