Technological Characterization and Identification of Potential Yeast Starters from Diospyros mespiliformis Fruit Pulp

SOUMAHORO Souleymane^1, , KOUAME Maimouna Liliane¹, COULIBALY Seydou Ouolouho², TOURE Abdoulaye³ and SORO Yadé René⁴

¹Laboratory of Biochemistry, Microbiology and Valorization of Agricultural Resources, Institute of Agropastoral Management University Peleforo Gon Coulibaly, PO Box 1328, Korhogo, Ivory Coast.

²Department of Biochemistry-Genetics, University Peleforo Gon Coulibaly (UPGC), Po Box 1328 Korhogo, Côte d’Ivoire.

³Laboratory of Biotechnology and Valorization of Agroresources and Natural Substances, Faculty of Biological Sciences, Peleforo Gon Coulibaly University, Korhogo, Korhogo, BP 1328, Côte d’Ivoire.

⁴Laboratory of Biotechnology, Training and Research Unit of Biosciences Faculty, Felix Houphouët Boigny University, P.O. Box 582 Abidjan 22, Ivory Coast

Cite this paper:
SOUMAHORO Souleymane, KOUAME Maimouna Liliane, COULIBALY Seydou Ouolouho, TOURE Abdoulaye and SORO Yadé René. Technological Characterization and Identification of Potential Yeast Starters from Diospyros mespiliformis Fruit Pulp. Journal of Applied & Environmental Microbiology. 2024; 12(1):1-6. doi: 10.12691/jaem-12-1-1

Abstract

The aim of this study was to add value to Diospyros Mespiliformis fruits from northern Côte d'Ivoire. Yeasts were isolated from Diospyros Mespiliformis pulp and screened to select potential starters. Following screening, the best strains were identified using the Maldi Tof technique to determine the species involved. The results enabled us to select three (3) strains with the best growth rates compared with the other strains tested. These strains belong to the species Candida krusei, Candida guillermondii and Candida fermentati. These strains could thus be used as potential starters in biotechnological applications. Diospyros Mespiliformis would be of interest in the development of yeast culture collections useful for controlled fermentations.

Keywords:
Diospyros Mespiliformis isolation screening starters yeasts

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References:

[1]	Chakravarty S., Bhutia K. D., Suresh C. P., Shukla G. and Pala N. A. (2016). A review on diversity, conservation and nutrition of wild edible fruits. Journal des sciences appliquées et naturelles, 8 (4), 2346-2353.
[2]	Kini F., Saba A., Ouédraogo S., Tinguéri B., Sanou G. and Guissou I. P. (2008). Potentiel nutritionnel et therapeutique de quelques especes fruitieres « sauvages » du Burkina Faso. Pharmacopée et Médecine Traditionnelle Africaines, 15, 32 – 35.
[3]	Daanon, A., Padonou, E. A., Akakpo, B. A. and Houinato, M. (2021). Variabilité des utilisations de Diospyros mespiliformis Hochst. Suivant les facteurs sociodémographiques au Nord-Bénin. Bois et Forêts des Tropiques, 347, 940.
[4]	Ambé G. (2001). Les fruits sauvages comestibles des savanes guinéennes de Côte-d’Ivoire : état de la connaissance par une population locale, les Malinkés. Biotechnologie, Agronomie, Société et Environnement, 5 (1), 43–58.
[5]	Ado A., Bil-Assanou I. H., Iro D. G., Karim T. D. A., Ali M. and Mahamane S. (2017). Effet de prétraitements, de substrats et de stress hydriques sur la germination et la croissance initiale de diospyros mespiliformis Hochst. Ex A. DC. European Scientific Journal, 13(21) : 231.
[6]	Desbordes D. (2003). Qualité microbiologique des fruits et légumes : flores, altérations, risques sanitaires, prévention. DESS Ingénierie documentaire, Rapport de Recherche Bibliographique, Ecole Nationale Supérieure des Sciences de l’Informations et des Bibliothèques (ENSSIB), 47 pages.
[7]	Ayed L., M’hir S. and Hamdi M. (2020). Aspects microbiologiques, biochimiques et fonctionnels des boissons fermentées de légumes et de fruits. Journal de la chimie,20 (2), 1-12.
[8]	Soumahoro S., Kouamé M.L., Samagaci L., Soro Y.R. and Marc L. (2024). Screening of Potential Yeast Starters from Cola cordifolia Fruit Pulp. American Journal of Food and Nutrition, 12(5), 127-133.
[9]	Samagaci, L., Ouattara, H. G., Goualié, B. G. and Niamke, S. L. (2014). Growth capacity of yeasts potential starter strains under cocoa fermentation stress conditions in Ivory Coast. Emirates Journal of Food and Agriculture, 26(10), 861-870.
[10]	Sidari, R., Ženišová, K., Tobolková, B., Belajová, E., Cabicarová, T., Buˇcková., M., Puškárová, A., Planý, M., Kuchta, T and Pangallo, D. (2021). Wine yeasts selection : Laboratory characterization and protocol review, Microorganisms, 9(11), 1-27.
[11]	Dung N. T. P and Phong. X.H. (2013). Screening thermo- and ethanol tolerant bacteria for ethanol fermentation. American Journal of Microbiological Research, 1(2), 25-31.
[12]	Dung N. T. P., Pornthap T. and Huynh X. P. (2012). Screening useful isolated yeasts for ethanol fermentation at high temperature. International Journal of Applied Science et Technology, 2 (4), 65-71.
[13]	Lemaresquier, H., Gainvors, A., Lequart, C., Charlemagne, B., Frézier, V. and Belarbi, A. (1995). Sélection de levures oenologiques á activité clarifiante. Revue Francaise d'œnologie 154, 23–29.
[14]	Nickerson, W. (1953). Reduction of inorganic substances by yeasts. I. Extracellular reduction of sulfite by species of Candida. The Journal of Infectious Diseases, 93, 43–56.
[15]	Caridi, A., Cufari, A. and Ramondino, D. (2002). Isolation and clonal pre-selection of enological Saccharomyces. The Journal of General and Applied Microbiology, 48, 261–267.
[16]	Comitini, F., Gobbi, M., Domizio, P., Romani, C., Lencioni, L., Mannazzu, I. and Ciani, M. (2011). Selected non-Saccharomyces wine yeasts in controlled multistarter fermentations with Saccharomyces cerevisiae. Food Microbiology, 28(5), 873–882.
[17]	Hollande, R.D., Wilkes, J.G., Rafii, F., Sutherland, J.B., Persons, C.C., Voorhees, K.J. and Lay Jr., J.O. (1996). Rapid identification of intact whole bacteria based on spectral patterns using matrixassisted laser desorption/ionization with time-of-flight mass spectrometry. Rapid Commun. Mass Spectrom. 10, 1227–1232.
[18]	Williams, A.G. and Banks, J.M. (2003). Proteolytic and other hydrolytic enzyme activities in non-starter lactic acid bacteria (NSLAB) isolated from Cheddar cheese manufactured in the United Kingdom. International Dairy Journal, 7, 763–774.
[19]	Chen, J. and Huo, G. (2008). Screening and identification of thermostable alphagalactosidaseproducing lactic acid bacteria and characteristics of its enzyme. In Abstracts / Journal of Biotechnology 136, 717–742.
[20]	Tokindrainy A. J. C. (2015). Isolement et identification des levures du fruit du bibacier de la region vakinankaratra. Memoire de master pour l’obtention de diplôme d’étude approfondie en sciences de la vie. Université d’Antanariovo, Madagascar, P.42.
[21]	Guiraud J.P. (1998). Microbiologie alimentaire. Dunod, Paris, P.696.
[22]	Holzapfel W. H. (2002). Appropriate starter culture technologies for small-scale fermentation in developing countries. International journal of Food Microbiology, 75 (3), 197-212.
[23]	Lambrechts, M.G. and Pretorius, I.S. (2000). Yeast and its importance to wine aroma-A review. South African Journal of Enology and Viticulture, 21(1), 97–129.
[24]	OIV. (2010). International Code of Oenological Practices ; Office Internationale de la Vigne et du Vin : Paris, France.
[25]	Bisson, L.F., Karpel, J.E., Ramakrishnan, V. and Joseph, L. (2007). Functional genomics of wine yeast Saccharomyces cerevisiae. Advances in Food and Nutrition Research 53, 65–121.
[26]	Mestre Furlani, M.V., Maturano, J.P., Combina, M., Mercado, L.A., Toro, M.E. and Vazquez, F. (2017). Selection of non-Saccharomyces yeasts to be used in grape musts with high alcoholic potential: A strategy to obtain wines with reduced ethanol content. FEMS Yeast Research, 17(2), 1–10.
[27]	Barbosa, C., Lage, P., Esteves, M., Chambel, L., Mendes-Faia, A. and Mendes-Ferreira, A. (2018). Molecular and phenotypic characterization of Metschnikowia pulcherrima strains from Douro wine region. Fermentation, 4(1), 1-19.
[28]	Garavaglia, J., de Cassia de Souza Schneider, R., Camargo Mendes, S.D., Welke, J.E., Alcaraz-Zini, C., Bastos Caramão, E. and Valente, P. (2015). Evaluation of Zygosaccharomyces bailii BCV 08 as a co-starter in wine fermentation for the improvement of ethyl esters production. Microbiological Research, 173, 59–65.
[29]	Da Silva R. O., Batistote M. and Cereda M. P. (2013). Alcoholic fermentation by the wild yeasts under thermal, osmotic and ethanol stress. Brazilian Archives of Biology and Technology, 56, 161–169.
[30]	Kim I. S., Yun H. S., Park I. S., Sohn H.Y., Iwahashi H. and Jin I. N. (2006). "A knockout strain of CPR1 induced during fermentation of Saccharomyces cerevisiae KNU5377 is susceptible to various types of stress." Journal of Bioscience and Bioengineering 102(4), 288-296.
[31]	Aldiguier A. S., Alfenore S., Cameleyre X., Goma G., Uribelarrea J. L., Guillouet S. E. and Molina-Jouve C. (2004). Synergistic temperature and ethanol effect on Saccharomyces cerevisiae dynamic behaviour in ethanol bio-fuel production. Bioprocess And Biosystems Engineering, 26(4), 217-222.
[32]	Khattak W. A., Ul-Islam M., Ullah M. W., Yu B., Khan S.b and Park J. K. (2014). "Yeast cell-free enzyme system for bio-ethanol production at elevated temperatures." Process Biochemistry 54(3), 357-364.
[33]	Ouédraogo N., Savadogo A., Somda M. K., Zongo C. and Traore A. S. (2012). Essai de production de protéines d’organismes unicellulaires par des souches de levures à partir de résidus de tubercules de patate douce et d’igname. Biotechnology, Agronomy and Society and Environment 16(4), 463-467.
[34]	Yeo, M. A., Kone, M. B., Koffi, E. K. and Coulibaly, L. (2021). Evaluation des caractéristiques, morphologiques physico-chimiques et sensorielles de la purée de deux variétés de tomates locales produites à petite échelle à Man (Côte d’Ivoire). International Journal Of Biological And Chemical Sciences, 15(2), 622634.
[35]	Fleet, G.H. (2003). Yeast interactions and wine flavour. International Journal of Food Microbiology, 86(1-2), 11–22.
[36]	Ženišová, K., Cabicarová, T., Sidari, R., Kolek, E., Pangallo, D., Szemes, T. and Kuchta, T. (2021). Effects of co-fermentation with Lachancea thermotolerans or Metschnikowia pulcherrima on concentration of aroma compounds in Pinot Blanc wine. Journal of Food and Nutrition Research, 60(1), 87–91.