Carbapenemase-producing Klebsiella pneumoniae Isolated from Environmental Sources in a Tertiary Health Institution in Nigeria

Iloduba Nnaemeka Aghanya^1, , Comfort Nne Akujobi¹, Simon Nkpeh Ushie¹, Chika Florence Ubajaka², Ijeoma Maryrose Ajuba³, Chibuike Jesse Ezeama¹, Nkechi Perpetua Maduekwe⁴, Ngozichukwu Gertrude Uzoewulu¹ and Chisom Godswill Chigbo⁵

¹Department of Medical Microbiology/Parasitology, Faculty of Medicine, Nnamdi Azikiwe University, Awka

²Department of Community Medicine, Faculty of Medicine, Nnamdi Azikiwe University, Nnewi Campus

³Department of Nursing Sciences, School of Nursing, Nnamdi Azikiwe University Teaching Hospital, Nnewi

⁴Department of Medical Microbiology/Parasitology, Nnamdi Azikiwe University Teaching Hospital, Nnewi

⁵Department of Applied Microbiology and Brewing, Nnamdi Azikiwe University, Awka

Cite this paper:
Iloduba Nnaemeka Aghanya, Comfort Nne Akujobi, Simon Nkpeh Ushie, Chika Florence Ubajaka, Ijeoma Maryrose Ajuba, Chibuike Jesse Ezeama, Nkechi Perpetua Maduekwe, Ngozichukwu Gertrude Uzoewulu and Chisom Godswill Chigbo. Carbapenemase-producing Klebsiella pneumoniae Isolated from Environmental Sources in a Tertiary Health Institution in Nigeria. Journal of Applied & Environmental Microbiology. 2021; 9(1):22-27. doi: 10.12691/jaem-9-1-4

Abstract

The acquisition of carbapenemase-producing organisms in healthcare settings is a significant threat and has dire implications for public health. Previous reports regarding carbapenemase-producing Enterobacteriaceae from fomites are limited. This study aimed to analyse the antimicrobial resistance patterns and prevalence of carbapenemase-producing Klebsiella pneumoniae in the ward environments of a tertiary health institution in Nigeria.One hundred and forty-two bacteria were isolated from 534 fomites in the hospital wards, and out of these, 15(10.6%) were K. pneumoniae. Therefore, the prevalence of K. pneumoniae in all the samples was 15/534(2.8%), while that of carbapenemase-producing K. pneumoniae was 8/534(1.5%). Multi-drug resistance was detected in 15/15(100%) of the K. pneumoniae isolated. All the K. pneumoniae isolates were resistant to ampicillin, trimethoprim-sulfamethoxazole, cefuroxime, and tetracycline. Although 8/15(53.3%) of the isolates were confirmed positive for carbapenemase production using the modified Hodge test, no Klebsiella pneumoniae carbapenemase gene (bla_KPC) was detected. The most frequent sites that harboured carbapenem-resistant K. pneumoniae were the beds 6/15(40%). Hence, the prevalence of carbapenemase-producing K. pneumoniae fomite colonisation in the NAUTH ward environment was low.

Keywords:
multidrug-resistant Klebsiella pneumoniae carbapenemase oxacillinase fomites

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